Aging effects in the extracellular matrix of the clitoris: A scanning electron microscopic analysis

Pires, Lucas Alves Sarmento; Babinski, Monique Silva Dias; Junior, Albino Fonseca; Manaia, Jorge Henrique Martin; Babinski, Márcio Antônio

doi:10.1016/j.morpho.2023.01.001

Cited by 3 publications

(1 citation statement)

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“…It is shown to stain collagens blue or green dependent on basic residues as well as muscle fibers red or yellow depending on their maturity [92]. Masson's Trichrome staining has been used to stain collagen and assess its structure and organization [93,94] and is oftentimes used alongside other ECM imaging techniques such as immunohistochemistry, second harmonic generation, and other imaging modalities [95–98].…”

Section: Spatial Analysis Of the Extracellular Microenvironmentmentioning

confidence: 99%

Molecular analysis of the extracellular microenvironment: from form to function

Macdonald,

Mehta,

Drake

et al. 2024

FEBS Letters

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The extracellular matrix (ECM) proteome represents an important component of the tissue microenvironment that controls chemical flux and induces cell signaling through encoded structure. The analysis of the ECM represents an analytical challenge through high levels of post‐translational modifications, protease‐resistant structures, and crosslinked, insoluble proteins. This review provides a comprehensive overview of the analytical challenges involved in addressing the complexities of spatially profiling the extracellular matrix proteome. A synopsis of the process of synthesizing the ECM structure, detailing inherent chemical complexity, is included to present the scope of the analytical challenge. Current chromatographic and spatial techniques addressing these challenges are detailed. Capabilities for multimodal multiplexing with cellular populations are discussed with a perspective on developing a holistic view of disease processes that includes both the cellular and extracellular microenvironment.

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Section: Spatial Analysis Of the Extracellular Microenvironmentmentioning

confidence: 99%