Agglutination potential of Pseudomonas fluorescens in relation to energy stress and colonization of Macrophomina phaseolina

Jana, Tarakanta; Srivastva, Alok K.; Csery, K.; Arora, Dilip K.

doi:10.1016/s0038-0717(99)00180-7

Cited by 7 publications

(5 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…might colonize the surface of the microsclerotia, but not the inner matrix. The adhesion of bacteria on the surface of the pathogen is an important feature in antagonistic interactions (Jana et al 2000) and our results support that this mechanism may play a role in the biocontrol activity of the tested Pseudomonas strains. However, the maximum Pseudomonas carrier capacity of the microsclerotia was approx.…”

Section: Microsclerotia Colonization and Competition For Nutrientssupporting

confidence: 83%

“…1999). For example, Jana et al. (2000) found that loss of nutrients from the sclerotia of Macrophomina phaseolina incubated with isolates of Ps.…”

Section: Introductionmentioning

confidence: 99%

“…Apart from the production of antifungal compounds, a second important mechanism by which bacterial antagonists may control soil pathogens, is that they may compete with the pathogen for nutrients, as both the pathogen and the bacterium can have similar carbon source nutrient utilization profiles (Ellis et al 1999). For example, Jana et al (2000) found that loss of nutrients from the sclerotia of Macrophomina phaseolina incubated with isolates of Ps. fluorescens was directly correlated with germination suppression.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Biosurfactants are involved in the biological control ofVerticilliummicrosclerotia byPseudomonasspp.

Debode

Maeyer

Perneel

et al. 2007

Journal of Applied Microbiology

View full text Add to dashboard Cite

Aims: To examine the effect of previously described bacterial antagonists on the viability of Verticillium microsclerotia in vitro and to elucidate the possible modes of action of bacterial strains in the suppression of Verticillium microsclerotia viability. Methods and Results: A microplate assay was developed to test the suppressive effect of well‐defined Pseudomonas spp. on the viability of Verticillium microsclerotia in vitro. Experiments using phenazine‐ and biosurfactant‐deficient mutants indicated that biosurfactants and phenazine‐1‐carboxylic acid play a role in the suppression of microsclerotia viability by Pseudomonas spp. In addition, microsclerotia colonization tests revealed that Pseudomonas spp. are able to colonize the surface of the microsclerotia, but not the inner matrix. Growth response curves showed that the population levels of Pseudomonas spp. increased when they were in the vicinity of Verticillium microsclerotia, indicating that Pseudomonas spp. may utilize nutrients from the microsclerotia for their growth. Conclusions: Pseudomonas spp. seem to be good candidates for Verticilllium microsclerotia biocontrol. Biosurfactant production is one of the main mechanisms involved in their mode of action. Significance and Impact of the Study: This line of work may contribute to a better understanding of biological control agents and their working mechanisms.

show abstract

Section: Microsclerotia Colonization and Competition For Nutrientssupporting

confidence: 83%

“…1999). For example, Jana et al. (2000) found that loss of nutrients from the sclerotia of Macrophomina phaseolina incubated with isolates of Ps.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Biosurfactants are involved in the biological control ofVerticilliummicrosclerotia byPseudomonasspp.

Debode

Maeyer

Perneel

et al. 2007

Journal of Applied Microbiology

View full text Add to dashboard Cite

show abstract

“…might colonize the surface of the microsclerotia, but not the inner matrix. The adhesion of bacteria on the surface of the pathogen is an important feature in antagonistic interactions (Jana et al, 2000) and our results support that this mechanism may play a role in the biocontrol activity of the tested Pseudomonas strains.…”

Section: Suppression Of Verticillium Wilt Of Olive By Bacteriasupporting

confidence: 83%

Suppression of Verticillium Wilt of Olive by Pseudomonas fluorescens

Sanei¹,

Razavi²

2011

AJEA

View full text Add to dashboard Cite

Protection of pathogen-free olive planting material from infection by Verticillium dahliae during plant propagation and/or at planting would help in the management of Verticillium wilt of olive. Despite the importance for rhizosphere functioning, rhizobacterial Pseudomonas spp. have been mainly studied in a cultivation-based manner. In this study, 8 isolates of Pseudomonas fluorescens obtained from roots of olive plants were tested for suppression of Verticillium wilt in nursery-produced olive planting stocks under controlled conditions. The antagonistic activity of P. fluorescens isolates from olive against defoliating (D) and nondefoliating (ND) V. dahliae pathotypes. The isolates of P. fluorescens isolates from olive varied in the ability to inhibit hyphal growth of D and ND V. dahliae on PDA. In planta bioassays were conducted under greenhouse conditions, by inoculating bacterial-treated and -nontreated 3-to 4-month-old, own-rooted plants of susceptible olive cv. Zard with the highly and less virulent V. dahliae. Results indicated that root treatment with some of P. fluorescens isolates significantly reduced the final disease incidence and severity, compared with the nontreated controls. Our results indicate that root treatment of olive plants with selected P. fluorescens isolates during nursery propagation can help in the biocontrol of V. dahliae in olive especially for ND pathotypes. No correlation was found between efficacy of tested bacterial isolates for in vitro antagonism of the pathogen and in planta suppression of Verticillium wilt.

show abstract

“…The ability of plant‐associated bacteria to colonize fungal hyphae is a fundamental ecological interaction (Gilbert and Linderman, 1971; Nesbitt et al ., 1981; Preece and Wong, 1982; Leben, 1984; Nelson et al ., 1986; Bryk et al ., 1998; Jana et al ., 2000; Hogan and Kolter, 2002; Bolwerk et al ., 2003), making it likely that the strategy of using fungi as a nutrient source may be common among bacteria found in fungal rich environments, like the rhizosphere or phyllosphere. However, the ability to colonize fungal hyphae is not a universal bacterial trait, as evidenced by the inability of E. coli to colonize N. crassa hyphae (Fig.…”

Section: Discussionmentioning

confidence: 99%

A novel gene, phcA from Pseudomonas syringae induces programmed cell death in the filamentous fungus Neurospora crassa

Wichmann

Sun

Dementhon³

et al. 2008

Molecular Microbiology

View full text Add to dashboard Cite

SummaryThe phytopathogen Pseudomonas syringae competes with other epiphytic organisms, such as filamentous fungi, for resources. Here we characterize a gene in P. syringae pv. syringae B728a and P. syringae pv. tomato DC3000, termed phcA, that has homology to a filamentous fungal gene called het-c. phcA is conserved in many P. syringae strains, but is absent in one of the major clades, which includes the P. syringae pathovar phaseolicola. In the filamentous fungus Neurospora crassa, HET-C regulates a conserved programmed cell death pathway called heterokaryon incompatibility (HI). Ectopic expression of phcA in N. crassa induced HI and cell death that was dependent on the presence of a functional het-c pin-c haplotype. Further, by co-immunoprecipitation experiments, a heterocomplex between N. crassa HET-C1 and PhcA was associated with phcA-induced HI. P. syringae was able to attach and extensively colonize N. crassa hyphae, while an Escherichia coli control showed no association with the fungus. We further show that the P. syringae is able to use N. crassa as a sole nutrient source. Our results suggest that P. syringae has the potential to utilize phcA to acquire nutrients from fungi in nutrientlimited environments like the phyllosphere by the novel mechanism of HI induction.

show abstract

Agglutination potential of Pseudomonas fluorescens in relation to energy stress and colonization of Macrophomina phaseolina

Cited by 7 publications

References 34 publications

Biosurfactants are involved in the biological control ofVerticilliummicrosclerotia byPseudomonasspp.

Biosurfactants are involved in the biological control ofVerticilliummicrosclerotia byPseudomonasspp.

Suppression of Verticillium Wilt of Olive by Pseudomonas fluorescens

A novel gene, phcA from Pseudomonas syringae induces programmed cell death in the filamentous fungus Neurospora crassa

Contact Info

Product

Resources

About