Age-related changes in haematology and serum chemistry of Weiser–Maples guineapigs (<i>Cavia porcellus</i>)

Kitagaki, Masato; Yamaguchi, Masatoshi; Nakamura, Minoru; Sakurada, Koichi; Suwa, Tetsuya; Sasa, Hitoshi

doi:10.1258/0023677054307042

Cited by 25 publications

(46 citation statements)

References 21 publications

(22 reference statements)

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“…The age-related reduction in the production of PCEs observed in our study is similar to that in rat (Kojima et al, 1999) and guineapigs (Kitagaki et al, 2005), in which the young animals showed the highest values of PCE in peripheral blood, suggesting an age-related decrease in hematopoiesis. The age-related reduction in the production of PCEs observed in our study is similar to that in rat (Kojima et al, 1999) and guineapigs (Kitagaki et al, 2005), in which the young animals showed the highest values of PCE in peripheral blood, suggesting an age-related decrease in hematopoiesis.…”

Section: Discussionsupporting

confidence: 85%

Influence of age and sex on the spontaneous DNA damage detected by Micronucleus test and Comet assay in mice peripheral blood cells

Heuser

Andrade

Peres

et al. 2008

Cell Biology International

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We have investigated the normal variations in basal DNA damage detected by Comet assay in leukocytes and micronucleated erythrocytes (MNE) using the Micronucleus test (MN) in peripheral blood cells from 45 female and male mice from different age groups (newborns, 3.5, 12, and 104 weeks) to clarify age and sex-related changes. Comparison of basal DNA damage detected by Comet assay showed significantly increased values in 104 weeks old mice in relation to the other ages (P < or = 0.01), and newborn mice showed higher values in MNE frequency when compared to all the other groups (P < or = 0.01). A positive correlation was observed between Damage Frequency (r =0.382, P = 0.010) and Damage Index (r = 0.640, P < 0.001) and age. Age was also correlated with the ratio of polychromatic erythrocytes/normachromatic erythrocytes (PCE/NCE) (r = -0.473, P = 0.001), and the MNE frequency was positively correlated with the ratio of PCE/NCE (r = 0.454, P = 0.002). These results suggest an age-related slow down of DNA repair efficiency of DNA damage and/or DNA damage accumulation. Furthermore, data on the spontaneous MNE frequency indicate that the reticuloendothelial system matures with age, and there is a close relationship between erythropoiesis and micronucleus induction in erythrocytes. The influence of sex in the parameters analyzed was less clear. In conclusion, age seems to influence in basal DNA damage and should be considered in genotoxicity studies using mice. Finally, comparisons between assays must be made with care when different cells are compared (e.g. leukocytes and erythrocytes), as found with the Comet assay and MN test.

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Section: Discussionsupporting

confidence: 85%

Influence of age and sex on the spontaneous DNA damage detected by Micronucleus test and Comet assay in mice peripheral blood cells

Heuser

Andrade

Peres

et al. 2008

Cell Biology International

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“…A general increase of total protein and globulin levels with increasing age has been reported in other species, including hystricomorph rodents (Kaneko 1997, Kitagaki and others 2005). …”

Section: Discussionsupporting

confidence: 52%

Selected haematological and plasma chemistry parameters in juvenile and adult degus (Octodon degus)

et al. 2011

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Thirty-five juvenile (mean age 6.3 weeks) and 35 adult (mean age 2.0 years) healthy degus (Octodon degus) were studied to investigate selected haematological and plasma biochemistry parameters. Animals were anaesthetised with isoflurane, and blood was withdrawn from the cranial vena cava. Erythrocyte, haematocrit and neutrophil counts (including the percentage of neutrophils) were significantly higher in the adult degus than in the juveniles. In contrast, the reticulocyte count, mean corpuscular volume, mean corpuscular haemoglobin, number of platelets and percentage of lymphocytes were significantly lower in the adult animals. Total protein and globulin levels were significantly higher in the adult degus. The albumin:globulin ratio and plasma levels of urea nitrogen, cholesterol, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, potassium, total calcium and inorganic phosphorus were significantly lower in adults than in juveniles.

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“…The ALP enzyme was found to decrease with body weight, as in other species (Clifford and White, 1999). Levels of ALP are reported to decrease sharply after birth and then level off in male and female guinea pigs of the inbred Weiser-Maples strain (Kitagaki et al, 2005).…”

Section: Alkaline Phosphatase (Alp)mentioning

confidence: 99%

Clinical Biochemistry and Hematology

Washington

Hoosier

2012

The Laboratory Rabbit, Guinea Pig, Hamster, and Other Rodents

111

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Age-related changes in haematology and serum chemistry of Weiser–Maples guineapigs (Cavia porcellus)

Cited by 25 publications

References 21 publications

Influence of age and sex on the spontaneous DNA damage detected by Micronucleus test and Comet assay in mice peripheral blood cells

Influence of age and sex on the spontaneous DNA damage detected by Micronucleus test and Comet assay in mice peripheral blood cells

Selected haematological and plasma chemistry parameters in juvenile and adult degus (Octodon degus)

Clinical Biochemistry and Hematology

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