Abstract:We have investigated the normal variations in basal DNA damage detected by Comet assay in leukocytes and micronucleated erythrocytes (MNE) using the Micronucleus test (MN) in peripheral blood cells from 45 female and male mice from different age groups (newborns, 3.5, 12, and 104 weeks) to clarify age and sex-related changes. Comparison of basal DNA damage detected by Comet assay showed significantly increased values in 104 weeks old mice in relation to the other ages (P < or = 0.01), and newborn mice showed h… Show more
“…They can also be caused by chemical reactions related to physiological processes. 9 In addition to age, studies have shown that differences in the occurrence of genetic damage can also be related to gender. [10][11][12] Trzeciak et al 11 reported that the removal of active carcinogens through tobacco is lower in women, thereby indicating biochemical differences between the genders.…”
The effects of aging, gender and lifestyle factors on inducing chromosomal damage (micronuclei) and nuclear degenerative changes were assessed using the micronucleus test on exfoliated cells of the oral mucosa. The sample included 80 healthy subjects divided into four groups according to age and gender: men and women aged 19-29 years (M19, W19) and men and women aged over sixty years (M60, W60). An interview questionnaire was used to characterize the sample and to determine an index reflecting lifestyle (HLI). The frequency of micronuclei and nuclear degenerative changes was significantly higher among the elderly ( p<0.001) and did not differ by gender among young people ( p>0.05). The occurrence of micronuclei was similar among elderly men and women ( p>0.10), but karyorrhexis and karyolysis were more frequent among men ( p<0.005 and p<0.025, respectively), who also had a lower HLI than the other groups ( p<0.0004). The results of the study indicate that age is the main factor associated with the induction of genetic material damage.
“…They can also be caused by chemical reactions related to physiological processes. 9 In addition to age, studies have shown that differences in the occurrence of genetic damage can also be related to gender. [10][11][12] Trzeciak et al 11 reported that the removal of active carcinogens through tobacco is lower in women, thereby indicating biochemical differences between the genders.…”
The effects of aging, gender and lifestyle factors on inducing chromosomal damage (micronuclei) and nuclear degenerative changes were assessed using the micronucleus test on exfoliated cells of the oral mucosa. The sample included 80 healthy subjects divided into four groups according to age and gender: men and women aged 19-29 years (M19, W19) and men and women aged over sixty years (M60, W60). An interview questionnaire was used to characterize the sample and to determine an index reflecting lifestyle (HLI). The frequency of micronuclei and nuclear degenerative changes was significantly higher among the elderly ( p<0.001) and did not differ by gender among young people ( p>0.05). The occurrence of micronuclei was similar among elderly men and women ( p>0.10), but karyorrhexis and karyolysis were more frequent among men ( p<0.005 and p<0.025, respectively), who also had a lower HLI than the other groups ( p<0.0004). The results of the study indicate that age is the main factor associated with the induction of genetic material damage.
“…Our results are in agreement with Joseph et al [19], who have stated an association between micronuclei and metastasis but no correlation with age or sex. However, Heuser et al [20] showed a positive relationship between spontaneous DNA damage in comet and micronuclei assays and age in mice.…”
Our results showed that there were no remarkable alterations either in the micronuclei incidence or in the percentage of apoptotic lymphocytes after in-vivo exposure to radiopharmaceutical imaging, which provides evidence to reduce the growing concern about the safety issue of cardiac imaging with Tc-MIBI, whereas the deleterious effects of I must be considered when it is applied to thyroid cancer treatment.
“…The Comet assay is used to detect primary DNA lesions (i.e., single or double strand breaks), while the MN assay is used to detect structural and numerical chromosomal damage. Additionally, the comet assay determines strand breaks and labile sites that are subsequently removed by repair enzymes (Heuser et al, 2008). In this study, both of the detecting techniques were successfully applied in detecting DNA damage in the two different drinking water treatment processes.…”
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