Age-dependent Changes of Differential Gene Expression Profile in Backfat Tissue between Hybrids and Parents in Pigs

Abstract

Large White, an introduced European pig breed, and Meishan, a Chinese indigenous pig breed, were hybridized directly and reciprocally and a total of 260 pigs, including purebreds, Large White and Meishan, and their hybrids, White×Meishan (LM) and Meishan×Large White (ML) pigs, were bred in our laboratory. The mRNA differential display PCR (DD-PCR) was used to detect the age-dependent changes of differential gene expression in backfat tissue between hybrids and parents. Some measures were taken to reduce the false positives in our experiment. Among the total of 2,686 bands obtained, 1,952 bands (about 72.67%) were reproducible and eight patterns (fifteen kinds) of gene expression were observed. The percentage of differentially expressed genes between hybrids and parents is 56.86% at the age of four months and 57.71% at the age of six months. This indicated that the differences of gene expression between hybrids and their parents were very obvious. U-test was used to compare the patterns of gene expression between the age of four and six months, and results showed that bands occurring in only one hybrid and bands displayed in one hybrid and one parent were significantly different at p<0.05, and bands visualized in only two hybrids were significantly different at p<0.01. These indicated that differential gene expression between hybrids and parents changed at different ages.

Cloning, Differential Expression, and Association Analysis with Fat Traits of Porcine IDH3γ Gene

“…Previously, we used mRNA differential display technique to analyze the differences of gene expression in backfat between Meishan and Large White pigs and their F1 hybrids, Large White×Meishan and Meishan×Large White pigs [10]. One potential differentially expressed EST, designated as ESTS30, was homologues to human γ subunit of NAD + -dependent isocitrate dehydrogenase gene (IDH3γ).…”

Identification of a differentially expressed gene,ACL, between Meishan × Large White and Large White × Meishan F1 hybrids and their parents

“…Since phenotypic variances mainly result from genotypic differences, it is necessary to provide experimental evidence for the genetic basis to differences between hybrids and their parents. In our laboratory, using suppression subtractive hybridization (SSH) and mRNA differential display, we have detected significant differences in mRNA quantities and expression patterns for several genes between porcine F1 hybrids and their parents [12,17,19]. Thus, cloning and characterizing genes that are differentially expressed between hybrids and their parents should provide further insights into the genetic basis of phenotypic differences.…”

“…Subsequently, cDNA sequences were synthesized with M-MLV reverse transcriptase and an oligo (dT)15 anchored primer (Promega, USA). Differential display PCR was carried out as described by Ren et al [17]. After differential display, cDNA fragments were re-amplified, cloned and sequenced.…”

Differential expression and effect of the porcine ANGPTL4 gene on intramuscular fat

“…Differential display PCR and non-denaturing polyacrylamide gel electrophoresis were employed as described by Ren et al (2005). The cDNA fragments differentially displayed on gel were re-amplified using the differential display primer set (Anchor3 and Arbitrary5; …”

“…In a previous study, we used an mRNA differential display technique to analyze differential gene expression in backfat between Meishan and Large White pigs and their F1 hybrids, Large White x Meishan and Meishan x Large White pigs (Ren et al, 2005). Many expressed sequence tags were isolated and identified.…”