Differential expression and effect of the porcine ANGPTL4 gene on intramuscular fat

Ren, Zhuqing; Wu, Wangjun; Liu, W H; Zheng, Rencheng; Li, J L; Zuo, Bo; Xu, Dequan; Li, F E; Lei, Minggang; Zhi, Yu; Xiong, Yan

doi:10.4238/2014.april.16.3

Cited by 15 publications

(12 citation statements)

References 26 publications

(27 reference statements)

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“…ANGPTL4 is mainly expressed in liver and adipose tissues [38] and plays important roles in the regulation of lipid metabolism. Previous studies have suggested that a SNP in ANGPTL4 is associated with intramuscular fat [39] and the expression level of ANGPTL4 affects meat quality traits of pigs [40]. Recent studies have shown that acyl-CoA synthetase family member 3 (ACSF3), in which DMRs in promoter region were found in the present study to be negatively correlated with the gene expression level, plays a critical role in mammal fatty acid synthesis in mitochondrion [41].…”

Section: Discussionmentioning

confidence: 51%

Comparative genome-wide methylation analysis of longissimus dorsi muscles between Japanese black (Wagyu) and Chinese Red Steppes cattle

Fang

Zhao

et al. 2017

PLoS ONE

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DNA methylation is an important epigenetic mechanism involved in expression of genes in many biological processes including muscle growth and development. Its effects on economically important traits are evinced from reported significant differences in meat quality traits between Japanese black (Wagyu) and Chinese Red Steppes cattle, thus presenting a unique model for analyzing the effects of DNA methylation on these traits. In the present study, we performed whole genome DNA methylation analysis in the two breeds by whole genome bisulfite sequencing (WGBS). Overall, 23150 differentially methylated regions (DMRs) were identified which were located in 8596 genes enriched in 9922 GO terms, of which 1046 GO terms were significantly enriched (p<0.05) including lipid translocation (GO: 0034204) and lipid transport (GO: 0015914). KEGG analysis showed that the DMR related genes were distributed among 276 pathways. Correlation analysis found that 331 DMRs were negatively correlated with the expression levels of differentially expressed genes (DEGs) with 21 DMRs located in promoter regions. Our results identified novel candidate DMRs and DEGs correlated with meat quality traits, which will be valuable for future genomic and epigenomic studies of muscle development and for marker assisted selection of meat quality traits.

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Section: Discussionmentioning

confidence: 51%

Comparative genome-wide methylation analysis of longissimus dorsi muscles between Japanese black (Wagyu) and Chinese Red Steppes cattle

Fang

Zhao

et al. 2017

PLoS ONE

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“…The previous studies have showed that ANGPTL4 was mainly expressed in liver, adipose tissue, placenta and ischemic tissues [ 15 , 16 , 22 ]. It was directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity.…”

Section: Discussionmentioning

confidence: 99%

“…The role of ANGPTL4 involved in the regulation of angiogenesis remains controversial [ 15 , 22 , 23 ]. It has been demonstrated that ANGPTL4 could inhibit VEGF-induced cell proliferation, migration, and tubule formation in HUVECs and this effect was via the suppression of Raf/MEK/ERK signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

TGF-β2-induced ANGPTL4 expression promotes tumor progression and osteoclast differentiation in giant cell tumor of bone

Qian

Cao

et al. 2017

Oncotarget

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Although emerging studies have implicated that Aiopoietin-like 4 Protein (ANGPTL4) is related to the aggressiveness and metastasis of many tumors, the role of ANGPLT4 in giant cell tumor (GCT) of bone was rarely investigated. The mechanism of ANGPLT4 in tumor-induced osteoclastogenesis still remains unclear. In this study, we first demonstrated that ANGPTL4 was highly expressed in GCT compared to normal tissues, while we showed that TGF-β2 released by osteoclasts induced bone resorption could increase the expression of ANGPTL4 in GCTSCs. By using the luciferase reporter assay, we found that two downstreams of TGF-β2, Smad3 and Smad4, could directly activate the promoter of ANGPTL4, which might explain the mechanism of TGF-β2-induced ANGPLT4 expression. Moreover, knockout of ANGPTL4 by TALENs in GCTSCs inhibited tumor growth, angiogenesis and osteoclastogenesis in GCT in vitro. By using the chick chorio-allantoic membrane (CAM) models, we further showed that inhibition of ANGPTL4 suppressed tumor growth and giant cell formation in vivo. In addition, some new pathways involved in ANGPTL4 application were identified through microarray assay, which may partly explain the mechanism of ANGPTL4 in GCT. Taken together, our study for the first time identified the role of ANGPLT4 in GCT of bone, which may provide a new target for the diagnosis and treatment of GCT.

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“…This approach identified the gene set that best correlates to IMF% is triacylglyceride (TAG) deposition, and includes FABP4. A number of other groups have identified similar sets of genes in various production species based on similar analyses performed on similar experimental designs (Saez et al, 2009;Li et al, 2013;Wang et al, 2013;Ren et al, 2014). However, to date the simplest measures of DE and correlations of abundance to phenotype have yielded only modest regulatory insight.…”

Section: Introductionmentioning

confidence: 99%

Longitudinal muscle gene expression patterns associated with differential intramuscular fat in cattle

Hudson

Reverter

Greenwood

et al. 2015

Animal

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Intramuscular fat (IMF) can improve meat product quality through its impact on flavour and juiciness. High marbling cuts can command premium prices in some countries and grading systems, but there is substantial cost involved in choosing to grain feed animals in an effort to deposit more IMF. There would be value in developing methods to predict predisposition to 'marble' well. Unfortunately, the biological mechanisms underpinning marbling remain a mystery: the key adipocyte cell populations have not been defined, there are no reliable DNA markers, no known (if any) causal mutations and gene expression analyses in the main have tended to characterise increases in expression of end-point fat metabolism proteins such as the fatty acid-binding proteins. To shed light on expression-based markers of marbling potential, we contrasted LD gene expression in high IMF Wagyu cross animals with a low IMF Piedmontese cross at various time points. The expected divergence in the fat metabolism genes FABP4, THRSP, CIDEC and ACACA between the breeds occurs surprisingly late in postnatal development at about 20 months. On the other hand, divergent expression of WISP2, RAI14 and CYP4F2 was discovered in animals at or before 12 months of age, suggesting these genes may have potential as earlier predictors of marbling potential. In line with other researchers, we found intriguing links between IMF development and connective tissue remodelling. WISP2 -a novel adipokine highly expressed and secreted by adipose precursor cells and an inhibitor of the pro-fibrotic connective tissue growth factor -emerges as a particularly attractive candidate. It is relatively upregulated in high marbling Wagyu before admission to feedlotting, somewhere between 7 and 12 months. This difference is subsequently maintained until 25 months, but not thereafter. RAI14, thought to play a role in porcine adipocyte differentiation and with links to retinoic acid metabolism, has an unusual expression profile. Its expression level increases monotonically with postnatal development, and is always higher in Wagyu than Piedmontese. Strong, sustained upregulation of the anti-inflammatory CYP4F2 in Piedmontese is consistent with Wagyu adiposity being a pro-inflammatory state. Application of regulatory impact factor analysis, a network method for identifying causal effector molecules, suggests marbling roles for transcription factors previously implicated in (1) the formation of liposarcoma (unconstrained fatty masses) (YEATS4, MDM2), (2) adipogenesis (CREBL2, SP1, STAT1) and (3) inflammation (ISGF3G, HOXB13, PML).

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Differential expression and effect of the porcine ANGPTL4 gene on intramuscular fat

Cited by 15 publications

References 26 publications

Comparative genome-wide methylation analysis of longissimus dorsi muscles between Japanese black (Wagyu) and Chinese Red Steppes cattle

Comparative genome-wide methylation analysis of longissimus dorsi muscles between Japanese black (Wagyu) and Chinese Red Steppes cattle

TGF-β2-induced ANGPTL4 expression promotes tumor progression and osteoclast differentiation in giant cell tumor of bone

Longitudinal muscle gene expression patterns associated with differential intramuscular fat in cattle

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