Age-dependent alterations in mRNA level and promoter methylation of collagen α1(I) gene in human periodontal ligament

Tomita, Masaki; Uyeno, Shinji; Komura, Jun‐ichiro; Watanabe, Makoto; Ono, Tetsuya

doi:10.1016/s0047-6374(99)00041-x

Cited by 37 publications

(31 citation statements)

References 29 publications

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“…Hypermethylation of a number of specific genes has been associated with cross-sectional age including the estrogen receptor (ER) (human colonic mucosa), 17 22 and collagen α1 (periodontal ligament tissue). 23 In addition, genes associated with colorectal cancer, including MYOD and N33, have been associated with age-related partial methylation in normal colon mucosa. 24 A study of over 200 human tissue samples from 10 anatomic sites also found a significant association between aging and DNA methylation.…”

Section: Discussionmentioning

confidence: 99%

Aging and epigenetics: Longitudinal changes in gene-specific DNA methylation

et al. 2012

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Section: Discussionmentioning

confidence: 99%

Aging and epigenetics: Longitudinal changes in gene-specific DNA methylation

et al. 2012

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“…COL1A1 methylation has been noted in aging periodontal fibroblasts (40), in MOV virus integration (50 -52) and during development (53), but it has not been reported previously in human cancer cells. Because the COL1A2 RFX1 site is methylated in human cancer cells, the same cells were examined for methylation status at the ϩ6 CpG site in COL1A1.…”

mentioning

confidence: 98%

Collagen α1(I) Gene (COL1A1) Is Repressed by RFX Family

Sengupta

Xu²,

Wang³

et al. 2005

Journal of Biological Chemistry

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Collagen type I is composed of three polypeptide chains transcribed from two separate genes (COL1A1 and COL1A2) with different promoters requiring coordinate regulation. Our recent publications, centering on COL1A2 regulation, demonstrate that methylation in the first exon of COL1A2 at a regulatory factor for X box (RFX) site (at ؊1 Collagen, a major extracellular matrix component, plays a significant role influencing cell behavior and maintaining tissue structure (1). Type I collagen, the most abundant member of the collagen family, is composed of two identical ␣1(I) polypeptide chains and a similar, but distinct polypeptide chain designated ␣2(I). Collagen expression is controlled in a developmental and tissue-specific manner. In adult tissue, type I gene expression is largely inactivated, but stimulated after injury. The two type I collagen genes (COL1A1 and COL1A2) are within separate chromosomes with different regulatory sequences in their promoters, yet they need to be regulated coordinately to ensure proper function.toSynthesis of collagen by cells in culture is down-regulated upon oncogenic transformation with viruses or chemical carcinogens (2-5). We have previously demonstrated (5) that collagen ␣2(I) is not expressed in a tumorigenic cell line, W8, after treatment of the parental liver epithelial-like cell line, K16, with the carcinogen 2-N-(acetoxyacetyl)-aminofluorine. Analysis of genomic DNA isolated from W8 cells revealed that the promoter-5Ј region of the ␣2(I) gene, COL1A2, was methylated (6). Furthermore, reporter constructs containing the COL1A2 promoter region (218 bp), as well as the first exon (54 bp) of the COL1A2 gene, were inactivated by DNA methylation in transient transfection experiments and in vitro transcription assays (6, 7). The inhibition of reporter gene expression was attributable to CpG methylation within the first exon surrounding the transcription start site of the COL1A2 gene. Finally, the collagen transcription start site (Ϫ1 to ϩ20) contains a low affinity-binding site for the regulatory factor for X box (RFX) family (8, 9). The binding affinity of RFX1 is increased if the CpG site at ϩ7 is methylated or mutated to thymidine on the coding strand.RFX1 is a member of the RFX family of closely related proteins, , that can bind methylated DNA sequences with higher affinity within a sequence-specific 14-bp consensus sequence. Methylation-dependent binding sites have been located for RFX at the beginning of human genes such as hypoxanthine phosphoribosyltransferase, ␣-galactosidase A, human leukocyte antigens, and the apoferritin H gene (14) suggesting a role for this protein family in DNA methylation induced gene repression. Most importantly, the RFX proteins repress COL1A2 gene expression in transient transfection as well as in vitro transcription assays (8).Our data suggest that RFX5 complex and CIITA play an important role in repression of COL1A2 gene when fibroblast cells are treated with IFN-␥ 1 (15, 16). RFX5, a member of the RFX family binds to DNA in a sequence-specifi...

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“…The tissue sample was extracted and processed by the method of Takatsu et al [18]. Both implants and teeth were unfrozen by water bath (bain-marie) at 37°C for 5 min.…”

Section: Processing Of Tissue Samplementioning

confidence: 99%

Role Of Advanced Glycation End Products (Ages) And Oxidative Stress In The Failure Of Dental Implants

Ramzan¹

2013

Dentistry

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Age-dependent alterations in mRNA level and promoter methylation of collagen α1(I) gene in human periodontal ligament

Cited by 37 publications

References 29 publications

Aging and epigenetics: Longitudinal changes in gene-specific DNA methylation

Aging and epigenetics: Longitudinal changes in gene-specific DNA methylation

Collagen α1(I) Gene (COL1A1) Is Repressed by RFX Family

Role Of Advanced Glycation End Products (Ages) And Oxidative Stress In The Failure Of Dental Implants

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