Age-associated development of inflammation in Wistar rats: Effects of caloric restriction

Abstract

These data indicate a role of adiposity in the development of inflammation and insulin resistance during ageing. Dietetic intervention could be a useful tool to ameliorate the development of inflammation and insulin resistance associated with ageing.

Anti-Inflamm-Aging Effects of Long-Term Caloric Restriction via Overexpression of SIGIRR to Inhibit NF-κB Signaling Pathway

“…Although increased TLR4 expression is typically thought of as equivalent to the activation of inflammation in theory, the limited studies that have been published on this topic to date have yielded discrepant results. For example, certain studies have reported no significant difference between CR and AL groups [21], whereas in the present study, the expression of TLR4 was significantly higher in the CR group than in the AL group. As it was possible that Xu et …”

Involvement of protein tyrosine phosphatases and inflammation in hypothalamic insulin resistance associated with ageing: Effect of caloric restriction

“…Activation of both PTP1B and LAR have been associated with inflammation and insulin resistant states (Cheung et al, 2000;Zabolotny et al, 2008). In this sense, hypothalamus from aged Wistar rats have been shown to present signs of inflammation such as increases in the expression of the pro-inflammatory cytokine TNFa, M1 macrophage marker CD11b and proteins related with macrophage infiltration and inflammation signaling such as chemo-attractant protein-1 (MCP1/CCL2) and toll like receptor-4 (TLR-4), respectively (Horrillo et al, 2011). On the other hand, our results that show increases in the amount of IKKb, p50 and p65 NFkb in both, cytosolic and nuclear fractions, together with the increase in the amount and phosphorylation of p38, support the activation of pro-inflammatory pathways, and reinforce the idea that different elements of inflammatory pathways seem to be up-regulated in hypothalamus of aged rats.…”

“…Moreover, these alterations have been shown to alter peripheral glucose metabolism and pancreatic function demonstrating the relationship between inflammation, central insulin resistance and peripheral glucose metabolism (Belgardt et al, 2010;Calegari et al, 2011). Similarly, aged Wistar rats have also shown to present signs of hypothalamic inflammation (Horrillo et al, 2011), increased IR and IRSs serine phosphorylation, as well as insulin and leptin resistance (Fernandez-Galaz et al, 2002;Garcia-San et al, 2007).…”

Effects of age and caloric restriction on the cardiac and coronary response to endothelin-1 in rats

“…Likewise the expression of these factors have been found to be increased both in the heart (Han et al, 2012) and in coronary arteries (Csiszar et al, 2002(Csiszar et al, , 2003 restriction for three months did not prevent aging-induced increase of iNOS and TNF-α. The same protocol of caloric restriction did not prevent aging-induced changes in the expression of TNF-α in other tissues such as the liver or the adipose tissue (Horrillo et al, 2011), which could be explained by either the small reduction in caloric intake (20%) or by the short duration of caloric restriction (3 months). Indeed, a more prolonged protocol of caloric restriction (10% during 9 months) or a severer reduction in food intake (40% during 10 days) significantly decreased the expression of several genes involved in inflammation in the serum (Spaulding et al, 1997), the adipose tissue (Higami et al, 2006), and the kidney (Jung et al, 2009) of aged animals.…”

“…This caloric restriction protocol has been developed in our laboratory for more than 20 years and is characterized for maintaining the proportions of food components without dietetic supplements. This protocol is considered as a bearable chronic dietetic intervention that renders aged animals with visceral adiposity index even lower than those of young ad libitum fed rats, being appropriate for studying the role of obesity in aging-associated physiological changes (Escriva et al, 1997;Horrillo et al, 2011). Caloric restricted animals were used at the age of 24 months, and killed by i.p.…”