Agaricus bisporus (Edible Mushroom Lectin) Inhibits Ocular Fibroblast Proliferation and Collagen Lattice Contraction

Batterbury, Mark; Tebbs, C.A.; Rhodes, Jonathan M.; Grierson, Ian

doi:10.1006/exer.2001.1133

Cited by 35 publications

(21 citation statements)

References 21 publications

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“…15,22 Previous studies indicated that such suppression did not result in cell death. 9,15,23 This finding was further confirmed by the absence of sub-G 1 (apoptotic) population in our flow cytometry studies. Through analyzing the cell cycle patterns, we demonstrated that ABL specifically suppressed the amount of cells present in S phase.…”

Section: Discussionsupporting

confidence: 74%

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Lectin fromAgaricus bisporusInhibited S Phase Cell Population and Akt Phosphorylation in Human RPE Cells

Cheung

Sheridan

et al. 2012

Invest. Ophthalmol. Vis. Sci.

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PURPOSE. Lectin from the edible mushroom Agaricus bisporus (ABL) was found to inhibit cell proliferation of some ocular and cancer cell lines. To elucidate how ABL inhibited RPE cell proliferation, we investigated the changes in cell cycle distribution and cell proliferation-related signaling pathways after ABL treatment.METHODS. Primary human RPE cells were isolated and grown in DMEM/F12 with or without the ABL (20 or 90 lg/mL) for 3 days. Analysis of cell cycle was performed by flow cytometry. Phosphorylation status of Erk, Jnk, p38, and Akt as well as p53 expression levels were investigated by Western blotting. The role of phosphorylated-Akt in RPE cell proliferation was further evaluated using LY294002. RESULTS.After ABL treatment (90 lg/mL), the amount of cells present in the S phase was found to be reduced. These changes were not apparent in cells treated with 20 lg/mL ABL. In addition, Erk and Akt were found to be hyperphosphorylated and hypophosphorylated, respectively. The expression levels of phosphorylated-Jnk, phosphorylated-p38, and p53 were not altered when compared with those of the control cells. When RPE cells were treated with LY294002 and deprived from phosphorylated-Akt expression, cell proliferation rate was reduced. Reduction in the amount of cells present in S phase was also observed. CONCLUSIONS.Our results showed that ABL hypophosphorylated Akt and this observation is in line with the fact that ABL attenuates cell proliferation. As the level of p53 was not significantly altered by ABL, this indicated that ABL-arrested cell cycle progression was independent of p53 activation. (Invest Ophthalmol Vis Sci. 2012; 53:7469-7475)

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Section: Discussionsupporting

confidence: 74%

“…[5][6][7][8] Specifically, lectin extracted from this edible mushroom, A. bisporus lectin (ABL), was found to inhibit cell proliferation of HT-29 colon cancer cells, primary human subconjunctival fibroblasts, and RPE cells. [9][10][11] However, little is known on how ABL suppresses cell proliferation.…”

mentioning

confidence: 99%

Lectin fromAgaricus bisporusInhibited S Phase Cell Population and Akt Phosphorylation in Human RPE Cells

Cheung

Sheridan

et al. 2012

Invest. Ophthalmol. Vis. Sci.

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“…One in vitro experiment was conducted to confi rm the inhibitory effect of ABL on fibroblasts found in the Tennon capsule. An increase in wound healing was shown, which furthermore led to the application of ABL in controlling scaring after surgical glaucoma treatment [Batterbury et al, 2002]. Lectins have also been examined as potentially anti-diabetic substances effective both in prophylaxis and treatment.…”

Section: Lectins From Fruiting Bodies Of a Bisporus And Their Antiprmentioning

confidence: 99%

Composition and Biological Properties of Agaricus bisporus Fruiting Bodies- a Review

Muszyńska

Kała

Rojowski

et al. 2017

Pol. J. Food Nutr. Sci.

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White Agaricus bisporus is both the most popular and the most commonly eaten edible mushroom species in the world. It is popular not only because of its taste, but also due to its high level of nutrients: dietary fi ber (chitin), essential, semi-essential amino acids, unsaturated fatty acids including linoleic and linolenic acids, easily digestible proteins, sterols, phenolic and indole compounds, and vitamins − especially provitamin D 2 and B 1 , B 2 , B 6 , B 7 , and C. Fruiting bodies of A. bisporus have antioxidant, antibacterial, anti-infl ammatory, antitumor, and immunomodulatory activity. The presence of antioxidant ergothioneine (which also displays the antimutagenic, chemo-and radioprotective activity) is also noteworthy. A. bisporus also contains derivatives of benzoquinone, a substance which belongs to the group of antibiotics. Studies of tyrosinase isolated from this species show its very high similarity to human tyrosinase. This points directly to the fact that this species could be a rich source of tyrosinase used for medicinal and cosmetics purposes. A. bisporus is also a rich source of selenium, zinc and other elements such as magnesium, copper, iron, potassium, sodium, calcium, phosphorus, sulfur or manganese. In conclusion, the presence of these compounds and elements with biological activity in fruiting bodies of A. bisporus confi rms their nutraceutical and medicinal properties. Unauthenticated Download Date | 5/12/18 5:17 AM

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“…Lectins have been isolated from fruit bodies and mycelium of many macromycete species. Use of fungal lectins in biological and medical research is possible due to their biological activity, mitogenic (She et al 1998;Wang et al 2004) and cytostatic, against a series of normal cells such as keratinocytes (Parslew et al 1999), iris fibroblasts (Batterbury et al 2002), and retinal pigment epithelial cells (Kent et al 2003). Recently studies on the antitumor activity of fungal lectins have appeared (Wang et al 2000;Liua et al 2001;Mahajan et al 2002;Ngai and Ng 2004;Wasser and Weis 1999;Wasser 2002).…”

Section: Mushroom Lectinsmentioning

confidence: 95%

Extracellular protein production and morphogenesis of Lentinula edodes in submerged culture

2009

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Along with a brief review of Lentinula edodes (shiitake mushroom) submerged cultivation history within the framework of important extracellular proteins biosynthesis, this study contains the authors' own results. The possibility of regulating the lectin activity of shiitake using the synthetic components is shown. The time course of lectin production in culture liquid of L. edodes in different media under submerged culture conditions was studied. The activity of agglutinins depended on the ratio between carbon and nitrogen sources and the pH of the culture medium. A relationship between the chemical composition of nutrient medium, the activity of extracellular lectins of L. edodes, and the formation of pigmented mycelial film in liquid culture has been found. The formulation of medium, on which the brown mycelial film appears in several days of submerged cultivation, is proposed. The results obtained make a contribution to the present notion of biochemical processes that give rise to the occurrence of the aforesaid morphological structure of shiitake. Finally, two extracellular lectins from the submerged culture of L. edodes have been isolated and purified to homogeneity. Their physicochemical properties and composition have been studied.

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Agaricus bisporus (Edible Mushroom Lectin) Inhibits Ocular Fibroblast Proliferation and Collagen Lattice Contraction

Cited by 35 publications

References 21 publications

Lectin fromAgaricus bisporusInhibited S Phase Cell Population and Akt Phosphorylation in Human RPE Cells

Lectin fromAgaricus bisporusInhibited S Phase Cell Population and Akt Phosphorylation in Human RPE Cells

Composition and Biological Properties of Agaricus bisporus Fruiting Bodies- a Review

Extracellular protein production and morphogenesis of Lentinula edodes in submerged culture

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