Lectin fromAgaricus bisporusInhibited S Phase Cell Population and Akt Phosphorylation in Human RPE Cells

Abstract

PURPOSE. Lectin from the edible mushroom Agaricus bisporus (ABL) was found to inhibit cell proliferation of some ocular and cancer cell lines. To elucidate how ABL inhibited RPE cell proliferation, we investigated the changes in cell cycle distribution and cell proliferation-related signaling pathways after ABL treatment.METHODS. Primary human RPE cells were isolated and grown in DMEM/F12 with or without the ABL (20 or 90 lg/mL) for 3 days. Analysis of cell cycle was performed by flow cytometry. Phosphorylation status of Erk, Jnk, p38, and Akt as well as p53 expression levels were investigated by Western blotting. The role of phosphorylated-Akt in RPE cell proliferation was further evaluated using LY294002. RESULTS.After ABL treatment (90 lg/mL), the amount of cells present in the S phase was found to be reduced. These changes were not apparent in cells treated with 20 lg/mL ABL. In addition, Erk and Akt were found to be hyperphosphorylated and hypophosphorylated, respectively. The expression levels of phosphorylated-Jnk, phosphorylated-p38, and p53 were not altered when compared with those of the control cells. When RPE cells were treated with LY294002 and deprived from phosphorylated-Akt expression, cell proliferation rate was reduced. Reduction in the amount of cells present in S phase was also observed. CONCLUSIONS.Our results showed that ABL hypophosphorylated Akt and this observation is in line with the fact that ABL attenuates cell proliferation. As the level of p53 was not significantly altered by ABL, this indicated that ABL-arrested cell cycle progression was independent of p53 activation. (Invest Ophthalmol Vis Sci. 2012; 53:7469-7475)

In vivo immunomodulatory effect of the lectin from edible mushroom Agaricus bisporus

“…28 T antigen and sialyl-T antigen are most important ligands of ABL. 15 Similar property was previously described for Galectin-1. ABL presence in in vitro assays inhibited 45% NO production, whereas in in vivo assays, when mice received oral ABL, NO production was 53% lower compared with mock treated animals.…”

“…5,6 High dietary intake of lectins such as wheat germ agglutinin, may affect the allergic response to oral antigens in the gut-associated lymphoid tissue. [13][14][15][16] On the other hand, ABL increases the pancreatic β-cell proliferation through regulation of cell cycle proteins. 8 The lectin from the edible mushroom Agaricus bisporus (ABL) has been studied for its capacity to bind with high affinity to Galβ3GalNAcαSer/Thr, core 1 mucin-type O-glycan residue, known as Thomsen-Friedenreich antigen (T antigen).…”

Medicinal mushrooms in adjuvant cancer therapies: an approach to anticancer effects and presumed mechanisms of action

“…Agaricus bisporus [20] Does lectin from A. bisporus inhibit cell proliferation of some ocular and cancer cell lines?…”

“…edodes C 91-3 is a mycelia fermentation protein isolated from fungi of Basidiomycetes Umbelliferae [20]. Its extract contains a variety of proteins with significant effects on inducing cell apoptosis confirmed by in vivo and in vitro experiments.…”

“…From a nutritional perspective and due to the high protein value and fiber content, cultivation of mushrooms has also been considered as an alternative to the supply of proteins in countries with high level of malnutrition [17]. Aside from this, edible mushrooms have been reported as having an antitumor potential [17][18][19] and anti-angiogenesis properties [20,21].…”