AFM and FluidFM Technologies: Recent Applications in Molecular and Cellular Biology

Amarouch, Mohamed Yassine; El-Hilaly, Jaouad; Mazouzi, Driss

doi:10.1155/2018/7801274

Cited by 29 publications

(20 citation statements)

References 44 publications

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“…Fluidic force microscopy (FluidFM) is a derivative of a conventional AFM where the cantilever contains a hollow microchannel and the tip has an aperture for fluid transfer to and from the cell. 276,[304][305][306] The microchannel is connected to a pressure controller via a macrochannel drilled to the AFM probe holder. Once the tip is inserted into the cell, the application of a negative pressure to the microchannel withdraws fluids from the cell.…”

Section: Pressure-driven Flow 531 Fluidic Force Microscopymentioning

confidence: 99%

Microfluidic Surgery in Single Cells and Multicellular Systems

Zhang¹,

Nadkarni²,

Paul³

et al. 2021

Preprint

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Microscale surgery on single cells and small organisms have enabled major advances in fundamental biology and in engineering biological systems. Examples of applications range from wound healing and regeneration studies to the generation of hybridoma to produce monoclonal antibodies. Even today, these surgical operations are often performed manually, but they are labor-intensive and lack reproducibility. Microfluidics has emerged as a powerful technology to control and manipulate cells and multicellular systems at the micro- and nanoscale with high precision. Here, we review the physical and chemical mechanisms of microscale surgery, and the corresponding design principles, applications, and implementations in microfluidic systems. We consider four types of surgical operations: 1) Sectioning, which splits a biological entity into multiple parts, 2) ablation, which destroys part of an entity, 3) biopsy, which extracts materials from within a living cell, and 4) fusion, which joins multiple entities into one. For each type of surgery, we summarize the motivating applications and the microfluidic devices developed. Throughout the review, we highlight existing challenges and opportunities. We hope that this review will inspire scientists and engineers to continue to explore and improve microfluidic surgical methods.

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Section: Pressure-driven Flow 531 Fluidic Force Microscopymentioning

confidence: 99%

Microfluidic Surgery in Single Cells and Multicellular Systems

Zhang¹,

Nadkarni²,

Paul³

et al. 2021

Preprint

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“…While the tip is scanning over the sample surface, the interaction forces between the tip and the sample surface distort the cantilever. The distortion is monitored with a laser beam, and could be deduced into topographic image vs. relative position of the tip (Amarouch et al, 2018 ). Cargo, such as plasmid DNA or dyes, could be deliver into cell by loading them onto normal (Cuerrier et al, 2007 ) or sharpened AFM tips before its penetration through cell membrane (Obataya et al, 2005 ; Silberberg et al, 2013 ).…”

Section: Single-cell Sampling With Nanostructuresmentioning

confidence: 99%

“…Fluidic force microscopy (FluidFM), is another powerful tools for the single cell sampling, which combines a conventional AFM with microchannel cantilevers connected to a pressure controlled fluidic circuit, and able to manipulate liquid locally (Guillaume-Gentil et al, 2014 ; Amarouch et al, 2018 ). Quantitative extraction of samples from single cells with subcellular spatiotemporal control was demonstrated using FluidFM, and meanwhile, the soluble molecules withdrawn from the cytoplasm or nucleus could be analyzed by transferring the extract sample fluid to TEM, enzyme activity assays, and qPCR (Guillaume-Gentil et al, 2014 ; Amarouch et al, 2018 ). The activities of the extract samples were monitored with microscopy, and the volumes were calculated from the size of occupied microchannels with typical volumes ranging from 0.1 to 7.0 in a single cell, as shown in Figure 2 (reprinted from Guillaume-Gentil et al, 2016 ).…”

Section: Single-cell Sampling With Nanostructuresmentioning

confidence: 99%

“…More recently, nanopipette has shown great potential in DNA detection in vitro , it also demonstrates the ability to sample from nucleoplasm or cytoplasm (Wang et al, 2019 ). Meanwhile, applications of AFM and FluidFM technologies in molecular and cellular biology have also been reviewed recently, especially in the aspects, such as cellular morphology, cellular mechanics, and manipulation of a single cell (Amarouch et al, 2018 ; Li et al, 2019 ). In this review, we focus on some most promising methods for single-cell sampling with nanostructures, such as glass nanopipette, nanostraw, carbon nanotube probes and other nanomaterial.…”

Section: Introductionmentioning

confidence: 99%

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The Most Recent Advances in the Application of Nano-Structures/Nano-Materials for Single-Cell Sampling

Jia

Chen

2020

Front. Chem.

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The research in endogenous biomolecules from a single cell has grown rapidly in recent years since it is critical for dissecting and scrutinizing the complexity of heterogeneous tissues, especially under pathological conditions, and it is also of key importance to understand the biological processes and cellular responses to various perturbations without the limitation of population averaging. Although conventional techniques, such as micromanipulation or cell sorting methods, are already used along with subsequent molecular examinations, it remains a big challenge to develop new approaches to manipulate and directly extract small quantities of cytosol from single living cells. In this sense, nanostructure or nanomaterial may play a critical role in overcoming these challenges in cellular manipulation and extraction of very small quantities of cells, and provide a powerful alternative to conventional techniques. Since the nanostructures or nanomaterial could build channels between intracellular and extracellular components across cell membrane, through which cytosol could be pumped out and transferred to downstream analyses. In this review, we will first brief the traditional methods for single cell analyses, and then shift our focus to some most promising methods for single-cell sampling with nanostructures, such as glass nanopipette, nanostraw, carbon nanotube probes and other nanomaterial. In this context, particular attentions will be paid to their principles, preparations, operations, superiorities and drawbacks, and meanwhile the great potential of nano-materials for single-cell sampling will also be highlighted and prospected.

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“…Two similar alternative approaches have been proposed for enabling intra-and extra-cellular delivery of biomolecules together with a variety of conventional AFM functions by employing newly designed AFM probes consisting of a microchanneled cantilever and a hollow tip with a submicrometer aperture instead of a conventional solid cantilever beam structure and solid sharp tip, to facilitate liquid handling [31,32]. Among them, fluidic force microscopy (FluidFM) is commercially available and provides an innovative platform in molecular and cellular biology [33,34].…”

Section: Introductionmentioning

confidence: 99%

Photocatalytic Nanofabrication and Intracellular Raman Imaging of Living Cells with Functionalized AFM Probes

et al. 2020

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Atomic force microscopy (AFM) is an effective platform for in vitro manipulation and analysis of living cells in medical and biological sciences. To introduce additional new features and functionalities into a conventional AFM system, we investigated the photocatalytic nanofabrication and intracellular Raman imaging of living cells by employing functionalized AFM probes. Herein, we investigated the effect of indentation speed on the cell membrane perforation of living HeLa cells based on highly localized photochemical oxidation with a catalytic titanium dioxide (TiO2)-functionalized AFM probe. On the basis of force–distance curves obtained during the indentation process, the probability of cell membrane perforation, penetration force, and cell viability was determined quantitatively. Moreover, we explored the possibility of intracellular tip-enhanced Raman spectroscopy (TERS) imaging of molecular dynamics in living cells via an AFM probe functionalized with silver nanoparticles in a homemade Raman system integrated with an inverted microscope. We successfully demonstrated that the intracellular TERS imaging has the potential to visualize distinctly different features in Raman spectra between the nucleus and the cytoplasm of a single living cell and to analyze the dynamic behavior of biomolecules inside a living cell.

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AFM and FluidFM Technologies: Recent Applications in Molecular and Cellular Biology

Cited by 29 publications

References 44 publications

Microfluidic Surgery in Single Cells and Multicellular Systems

Microfluidic Surgery in Single Cells and Multicellular Systems

The Most Recent Advances in the Application of Nano-Structures/Nano-Materials for Single-Cell Sampling

Photocatalytic Nanofabrication and Intracellular Raman Imaging of Living Cells with Functionalized AFM Probes

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