Photocatalytic Nanofabrication and Intracellular Raman Imaging of Living Cells with Functionalized AFM Probes

Shibata, Takayuki; Furukawa, Hiromi; Ito, Yasuharu; Nagahama, Masahiro; Hayashi, Terutake; Ishii-Teshima, Miho; Nagai, Masao

doi:10.3390/mi11050495

Cited by 8 publications

(4 citation statements)

References 57 publications

(84 reference statements)

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“…Probe geometry-forward, backward, side angles, and tip height-are also parameters that affect scanning resolution and limit the maximum detectable roughness of the sample surface. Moreover, tips could be coated with various materials to extend their application field [50].…”

Section: Cantileversmentioning

confidence: 99%

Characteristics and Functionality of Cantilevers and Scanners in Atomic Force Microscopy

Dzedzickis,

Rožėnė,

Bučinskas

et al. 2023

Materials

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In this paper, we provide a systematic review of atomic force microscopy (AFM), a fast-developing technique that embraces scanners, controllers, and cantilevers. The main objectives of this review are to analyze the available technical solutions of AFM, including the limitations and problems. The main questions the review addresses are the problems of working in contact, noncontact, and tapping AFM modes. We do not include applications of AFM but rather the design of different parts and operation modes. Since the main part of AFM is the cantilever, we focused on its operation and design. Information from scientific articles published over the last 5 years is provided. Many articles in this period disclose minor amendments in the mechanical system but suggest innovative AFM control and imaging algorithms. Some of them are based on artificial intelligence. During operation, control of cantilever dynamic characteristics can be achieved by magnetic field, electrostatic, or aerodynamic forces.

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Section: Cantileversmentioning

confidence: 99%

Characteristics and Functionality of Cantilevers and Scanners in Atomic Force Microscopy

Dzedzickis,

Rožėnė,

Bučinskas

et al. 2023

Materials

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“…They also looked at the potential for visualizing cells using TERS. This imaging is functionalized by using silver nanoparticles in a home-built Raman system combined with an inverted microscope through AFM probes, which successfully demonstrated that TERS imaging is capable of visualizing noticeably different features in the Raman spectrum between the nucleus and cytoplasm of a single living cell and of analyzing the dynamic behavior of biomolecules in living cells ( Shibata et al, 2020 ).…”

Section: Raman Spectroscopymentioning

confidence: 99%

Advances in the application of Raman spectroscopy in haematological tumours

Liang

Shi

Wang

et al. 2023

Front. Bioeng. Biotechnol.

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Hematologic malignancies are a diverse collection of cancers that affect the blood, bone marrow, and organs. They have a very unpredictable prognosis and recur after treatment. Leukemia, lymphoma, and myeloma are the most prevalent symptoms. Despite advancements in chemotherapy and supportive care, the incidence rate and mortality of patients with hematological malignancies remain high. Additionally, there are issues with the clinical diagnosis because several hematological malignancies lack defined, systematic diagnostic criteria. This work provided an overview of the fundamentals, benefits, and limitations of Raman spectroscopy and its use in hematological cancers. The alterations of trace substances can be recognized using Raman spectroscopy. High sensitivity, non-destructive, quick, real-time, and other attributes define it. Clinicians must promptly identify disorders and keep track of analytes in biological fluids. For instance, surface-enhanced Raman spectroscopy is employed in diagnosing gene mutations in myelodysplastic syndromes due to its high sensitivity and multiple detection benefits. Serum indicators for multiple myeloma have been routinely used for detection. The simultaneous observation of DNA strand modifications and the production of new molecular bonds by tip-enhanced Raman spectroscopy is of tremendous significance for diagnosing lymphoma and multiple myeloma with unidentified diagnostic criteria.

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“…Crucially, scientists have shown that the viability of the cell membrane is very high (about 100%) after the AFM tip penetrates the cell membrane. The method has significant potential for future use in studies where it is necessary to investigate the various organelles and biomolecules within the cell [75].…”

Section: Therapeutic Drug Monitoring (Tdm)mentioning

confidence: 99%

Raman Spectroscopy and Its Modifications Applied to Biological and Medical Research

Allakhverdiev

Khabatova

Kossalbayev

et al. 2022

Cells

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Nowadays, there is an interest in biomedical and nanobiotechnological studies, such as studies on carotenoids as antioxidants and studies on molecular markers for cardiovascular, endocrine, and oncological diseases. Moreover, interest in industrial production of microalgal biomass for biofuels and bioproducts has stimulated studies on microalgal physiology and mechanisms of synthesis and accumulation of valuable biomolecules in algal cells. Biomolecules such as neutral lipids and carotenoids are being actively explored by the biotechnology community. Raman spectroscopy (RS) has become an important tool for researchers to understand biological processes at the cellular level in medicine and biotechnology. This review provides a brief analysis of existing studies on the application of RS for investigation of biological, medical, analytical, photosynthetic, and algal research, particularly to understand how the technique can be used for lipids, carotenoids, and cellular research. First, the review article shows the main applications of the modified Raman spectroscopy in medicine and biotechnology. Research works in the field of medicine and biotechnology are analysed in terms of showing the common connections of some studies as caretenoids and lipids. Second, this article summarises some of the recent advances in Raman microspectroscopy applications in areas related to microalgal detection. Strategies based on Raman spectroscopy provide potential for biochemical-composition analysis and imaging of living microalgal cells, in situ and in vivo. Finally, current approaches used in the papers presented show the advantages, perspectives, and other essential specifics of the method applied to plants and other species/objects.

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Photocatalytic Nanofabrication and Intracellular Raman Imaging of Living Cells with Functionalized AFM Probes

Cited by 8 publications

References 57 publications

Characteristics and Functionality of Cantilevers and Scanners in Atomic Force Microscopy

Characteristics and Functionality of Cantilevers and Scanners in Atomic Force Microscopy

Advances in the application of Raman spectroscopy in haematological tumours

Raman Spectroscopy and Its Modifications Applied to Biological and Medical Research

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