AFLP fingerprinting for paternity testing in ducks

Huang, Chang-Wen; Cheng, Y. S.; Rouvier, R.; Yang, Kuo Chung; Wu, Chien‐Ping; Huang, Mu-Chiou

doi:10.1080/00071660701370459

Cited by 14 publications

(6 citation statements)

References 38 publications

(50 reference statements)

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“…Increasing evidence [ 15 , 18 , 23 - 25 ] shows that the gender of some avian species cannot be identified by the P2/P8 PCR-based protocol [ 12 ] due to the limited difference in the length of intron for CHD-Z and CHD-W . This limitation has been overcome by some methods such as redesigned PCR primers [ 18 ] and our proposed approach, as well as PCR-RFLP [ 23 , 25 ], RAPD [ 26 ] and AFLP [ 27 ] fingerprintings. Some of these methods may also have the potential for high-throughput application of avian molecular sexing, but their gel electrophoresis step has to be improved.…”

Section: Discussionmentioning

confidence: 99%

High-throughput avian molecular sexing by SYBR green-based real-time PCR combined with melting curve analysis

Chang

Cheng

et al. 2008

BMC Biotechnol

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Section: Discussionmentioning

confidence: 99%

High-throughput avian molecular sexing by SYBR green-based real-time PCR combined with melting curve analysis

Chang

Cheng

et al. 2008

BMC Biotechnol

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“…Finally, although parentage analyses indicate reasonably consistent Mendelian segregation (e.g. Huang et al 2007), the unidominant nature of AFLPs makes it diYcult to evaluate deviations from Hardy-Weinberg equilibrium, a key step in the quality control process for codominant markers such as microsatellites. As with other markers including microsatellites (HoVman and Amos 2005), it is therefore strongly recommended that data quality be evaluated by replicating a randomly selected subset of samples from scratch (Bonin et al 2004).…”

Section: Aflp Ampliwcation and Levels Of Polymorphismmentioning

confidence: 99%

Widespread amplification of amplified fragment length polymorphisms (AFLPs) in marine Antarctic animals

2011

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Although recent years have witnessed a rapid growth in the number of genetic studies of Antarctic organisms, relatively few studies have so far used nuclear markers, possibly due to the perceived cost and diYculty of isolating markers such as microsatellites. However, an often overlooked alternative is to use ampliWed fragment length polymorphisms (AFLPs), a versatile and low-cost method capable of generating large numbers of predominantly nuclear loci in virtually any organism. We conducted a literature review of population genetic studies of Antarctic organisms, Wnding that fewer than 10% used AFLPs. Moreover, a strong taxonomic bias was found, with studies employing mitochondrial DNA or microsatellites focussing predominantly on animals, while those using AFLPs were mostly of plants or lower organisms. Consequently, we explored the extent to which AFLPs amplify across a range of Antarctic marine animal taxa by genotyping eight individuals each of twelve diVerent species, ranging from echinoderms through soft corals to pelagic Wsh, at four selective primer combinations. AFLPs readily ampliWed across all of the taxa, generating between 32 and 84 loci per species, with on average 56.5% of these being polymorphic. In general, levels of polymorphism bore little relationship with expectations based on larger populations of broadcastspawning species being more variable, though we did Wnd a tentative positive correlation between the number of AFLP bands ampliWed and a measure of eVective population size. Our study lends further support for the utility and ease of use of AFLPs and their suitability for studies of Antarctic species across a wide range of taxa.

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“…1995), which can effectively detect DNA polymorphisms without prior information on the genome (Brugmans et al. 2003; Huang et al. 2007; Negrini et al.…”

Section: Introductionmentioning

confidence: 99%

Development of SNP markers for individual identification and parentage test in a Japanese Black cattle population

Hara

Watabe

Sasazaki

et al. 2010

Animal Science Journal

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This study describes the development of efficient single nucleotide polymorphism (SNP) markers for individual identification and parentage tests in a Japanese Black cattle population. An amplified fragment length polymorphism method was employed to detect informative candidate markers, and yielded 44 SNP markers from 220 primer combinations. 29 unlinked SNPs were finally selected as diagnostic markers. The allelic frequencies for each marker were estimated by using PCR-RFLP in the Japanese Black population. Based on the frequency data, the estimated identity power of these markers was 2.73 x 10(-12). Parentage exclusion probabilities, when both suspected parents' genotypes were known and when only one suspected parent was genotyped, were estimated as 0.96929 and 0.99693, respectively. This panel of SNP markers is theoretically sufficient for individual identification, and would also be a powerful tool for a parentage test in Japanese Black cattle. The markers could contribute to the management of the beef industry in Japan.

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AFLP fingerprinting for paternity testing in ducks

Cited by 14 publications

References 38 publications

High-throughput avian molecular sexing by SYBR green-based real-time PCR combined with melting curve analysis

High-throughput avian molecular sexing by SYBR green-based real-time PCR combined with melting curve analysis

Widespread amplification of amplified fragment length polymorphisms (AFLPs) in marine Antarctic animals

Development of SNP markers for individual identification and parentage test in a Japanese Black cattle population

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