High-throughput avian molecular sexing by SYBR green-based real-time PCR combined with melting curve analysis

Chang, Hsueh-Wei; Cheng, Chun An; Gu, De Leung; Chang, Chia‐Che; Su, San Hua; Wen, Cheng; Chou, Y. C.; Chou, Ta Ching; Yao, Cheng Te; Tsai, Chi Li; Cheng, Chin‐Chi

doi:10.1186/1472-6750-8-12

Cited by 61 publications

(40 citation statements)

References 21 publications

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“…After real time PCR, melting curve analyses and gel electrophoreses were performed to ensure the specificity of the quantitative RT-PCR reactions (Chang et al, 2008;Yen et al, 2009). Herman et al, 1996) DNA was modified with 1 μg of genomic DNA using a CpGenomet DNA modification kit (Chemicon, Temecula, CA) (Shieh et al, 2005) …”

Section: Rna Extraction and Real Time Rt-pcrmentioning

confidence: 99%

The mitogen-activated protein kinase phosphatase-1 (MKP-1) gene is a potential methylation biomarker for malignancy of breast cancer

et al. 2012

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Section: Rna Extraction and Real Time Rt-pcrmentioning

confidence: 99%

The mitogen-activated protein kinase phosphatase-1 (MKP-1) gene is a potential methylation biomarker for malignancy of breast cancer

et al. 2012

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“…Alternative W-linked sequences that are more widely conserved have been utilised to sex a variety of avian species, from many different families. For example, the conserved W-linked gene, CHD-W (ChromoHelicase DNA-binding protein-W linked) has a homologue on the Z (CHD-Z) but the two sequences can be distinguished by different sized introns, or by the presence of unique restriction enzyme sites (Griffiths et al 1996;Chang et al 2008). This has allowed the use of the sex-linked CHD gene for the molecular sexing of several different birds, including rare species (Ellegren 1996;Griffiths et al 1998;Wang et al 2007).…”

Section: Avian Sex Chromosomes and Sex-determining Genes: Applicationmentioning

confidence: 99%

Rowley Review

Smith

2010

Emu - Austral Ornithology

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Abstract. In birds, sex determination occurs at fertilisation by the inheritance of sex chromosomes. This review summarises our current understanding of sex determination in birds, with emphasis on the molecular genetics of male versus female development during embryonic life. Recent studies in the Chicken (Gallus gallus domesticus) have revealed some remarkable features of avian sex determination, such as the finding that sex appears to be determined autonomously within cells throughout the body, and the demonstration that the key, sex-linked gene, DMRT1, is required for testis formation and hence male development. However, despite these recent advances, the mechanism of avian sex determination is still not entirely clear. Understanding sex determination in birds has important implications for the conservation of threatened species, and for the global poultry industry.

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“…A reliable molecular sexing method is required as the majority of tissue manipulations/collections are performed when male and female embryos are morphologically indistinguishable. There are currently a number of methods available, but these are laborious and time-consuming and incompatible with studies involving live embryos [Clinton, 1994;Griffiths et al, 1998;Cortes et al, 1999;Fridolfsson and Ellegren, 1999;Clinton et al, 2001;Chang et al, 2008]. To address this requirement, we have developed a simple and robust assay that allows the rapid identification of the sex of individual embryos using a small quantity of crude material.…”

Section: Real-time Sexing Of Chicken Embryos and Compatibility With Imentioning

confidence: 99%

Real-Time Sexing of Chicken Embryos and Compatibility with in ovo Protocols

Clinton

Nandi

Zhao

et al. 2016

Sex Dev

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The chicken embryo is an established model system for studying early vertebrate development. One of the major advantages of this model is the facility to perform manipulations in ovo and then continue incubation and observe the effects on embryonic development. However, in common with other vertebrate models, there is a tendency to disregard the sex of the experimental chicken embryos, and this can lead to erroneous conclusions, a lack of reproducibility, and wasted efforts. That this neglect is untenable is emphasised by the recent demonstration that avian cells and tissues have an inherent sex identity and that male and female tissues respond differently to the same stimulus. These sexually dimorphic characteristics dictate that analyses and manipulations involving chicken embryos should always be performed using tissues/embryos of known sex. Current sexing protocols are unsuitable in many instances because of the time constraints imposed by most in ovo procedures. To address this lack, we have developed a real-time chicken sexing assay that is compatible with in ovo manipulations, reduces the number of embryos required, and conserves resources.

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High-throughput avian molecular sexing by SYBR green-based real-time PCR combined with melting curve analysis

Cited by 61 publications

References 21 publications

The mitogen-activated protein kinase phosphatase-1 (MKP-1) gene is a potential methylation biomarker for malignancy of breast cancer

The mitogen-activated protein kinase phosphatase-1 (MKP-1) gene is a potential methylation biomarker for malignancy of breast cancer

Rowley Review

Real-Time Sexing of Chicken Embryos and Compatibility with in ovo Protocols

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