AFLP analysis of genetic relationships among papaya and its wild relatives (Caricaceae) from Ecuador

Abstract

The AFLP technique was used to assess the genetic relationships among the cultivated papaya ( Carica papaya L.) and related species native to Ecuador. Genetic distances based on AFLP data were estimated for 95 accessions belonging to three genera including C. papaya, at least eight Vasconcella species and two Jacaratia species. Cluster analysis using different methods and principal co-ordinate analysis (PCO), based on the AFLP data from 496 polymorphic bands generated with five primer combinations, was performed. The resulted grouping of accessions of each species corresponds largely with their taxonomic classifications and were found to be consistent with other studies based on RAPD, isozyme and cpDNA data. The AFLP analysis supports the recent rehabilitation of the Vasconcella group as a genus; until recently Vasconcella was considered as a section within the genus Carica. Both cluster and PCO analysis clearly separated the species of the three genera and illustrated the large genetic distance between C. papaya accessions and the Vasconcella group. The specific clustering of the highly diverse group of Vasconcella x heilbornii accessions also suggests that these genotypes may be the result of bi-directional introgression events between Vasconcella stipulata and Vasconcella cundinamarcensis.

Screening interspecific hybrids of Populus (P. ciliata × maximowiczii) using AFLP markers

“…This speciesspecific clustering pattern of the hybrids has also been observed in papaya (Van Droogenbroeck et al 2002) and coffee (Steiger et al 2002). Our results show the clustering of the P. ciliata maximowiczii hybrids with the two parent species, with the hybrid cluster being closer to the P. ciliata cluster.…”

Multivariate analysis to determine the genetic distance among backcross papaya (Carica papaya) progenies

“…Among all species, 32 are dioecious, two trioecious and one monoecious (Ming et al, 2007). The papaya is the only species of the genus Carica, also being the best known and most economically important within the family (Van Droogenbroeck et al, 2002), showing widespread cultivation in tropical and subtropical regions around the world. Its germplasm has considerable phenotypic variation for many characteristics of horticultural importance, including size and fruit shape, flesh color, flavor and soluble solids content, length of juvenile period, plant height, etc.…”

“…Some studies on papaya have reported intra-and inter-generic analysis using morphological data, isozyme, RFLP (restriction fragment length polymorphism), PCR-based molecular markers like AFLP (amplified fragment length polymorphism), RAPD (random amplified polymorphic DNA), ISSR (inter-simple sequence repeat), DAMD (directed amplification of minisatellite DNA), and microsatellites or SSR (simple sequence repeats) (Jobin-Decor et al, 1997;Parasnis et al, 1997;Van Droogenbroeck et al, 2002;Vitória et al, 2004;Kyndt et al, 2005;Saxena et al, 2005;Ocampo et al, 2006;Silva et al, 2007;Eustice et al, 2008;Oliveira et al, 2010). However, studies that use morpho-agronomic and molecular data together to assess the genetic material used in breeding programs are scarce.…”

A SCAR marker for the sex types determination in Colombian genotypes of Carica papaya

“…Papaya (Carica papaya L.) is a member of the Caricaceae, a small dicotyledonous family consisting of six genera of herbaceous, shrubby or arborescent plants (Van Droogenbroeck et al 2002) It is now the only species belonging to the genus Carica, since Badillo rehabilitated the Vasconcella group, until recently considered as a section within the genus Carica. Vasconcella, now comprising 21 species, is the largest genus of the family, followed by the genus Jacaratia with seven species (Van Droogenbroeck et al 2002).…”