Aflatoxins ingestion and canine mammary tumors: There is an association?

Exposure assessment of dogs to mycotoxins through consumption of dry feed

“…Limits of detection (LOD) and quantification (LOQ) were determined as described previously. 28 The LODs for FB 1 and FB 2 were 27.5 and 35.3 μg kg −1 and the LOQs for FB 1 and FB 2 were 45.8 and 58.8 μg kg −1 respectively. The recoveries of FB 1 and FB 2 from spiked dog feed in the range 100-400 μg kg −1 FB 1 and 250-450 μg kg −1 FB 2 averaged 95.9% (mean coefficient of variation (CV) 9%) and 94.2% (mean CV 12%) respectively based on duplicate spiking and triplicate analyses.…”

“…Limits of detection (LOD) and quantification (LOQ) were determined as described previously . The LODs for FB 1 and FB 2 were 27.5 and 35.3 µg kg −1 and the LOQs for FB 1 and FB 2 were 45.8 and 58.8 µg kg −1 respectively.…”

“…The LOD and LOQ for ZEA were determined as 3.95 and 6.08 µg kg −1 respectively. ZEA recovery rates from spiked dog feed in the range 100–400 µg kg −1 ZEA averaged 87% (mean CV 6.4%) based on duplicate spiking and triplicate analyses.…”

“…LODs were determined as 0.13 µg kg −1 (AFB 1 ), 0.03 µg kg −1 (AFG 1 ), 0.59 µg kg −1 (AFB 2 ) and 0.22 µg kg −1 (AFG 2 ). LOQs were determined as 0.32 µg kg −1 (AFB 1 ), 0.15 µg kg −1 (AFG 1 ), 1.09 µg kg −1 (AFB 2 ) and 0.48 µg kg −1 (AFG 2 ).…”

“…LODs were determined as 0.13 µg kg −1 (AFB 1 ), 0.03 µg kg −1 (AFG 1 ), 0.59 µg kg −1 (AFB 2 ) and 0.22 µg kg −1 (AFG 2 ). LOQs were determined as 0.32 µg kg −1 (AFB 1 ), 0.15 µg kg −1 (AFG 1 ), 1.09 µg kg −1 (AFB 2 ) and 0.48 µg kg −1 (AFG 2 ). Aflatoxin recovery rates from spiked dog feed in the range 10.0–100 µg kg −1 (sum of the four analogues) averaged 90% (CV 5.22–13.05%), while mean recovery rates for each analogue spiked at concentrations from 2.50 to 25.0 µg kg −1 were 89% for AFB 1 , 99% for AFG 1 , 90% for AFB 2 and 82% for AFG 2 (mean CV 7.75%).…”