Aflatoxin B1-induced micronuclei and cell cycle alterations in lung and bone marrow cells and their modulation byPiper argyrophyllumextract

Raj, Hanumantharao G.; Gupta, Kshitij; Rohil, Viswajeet; Bose, Mridula; Biswas, Gopa; Singh, Sanjay K.; Jain, Subhash C.; Parmar, Virinder S.; Olsen, Carl Erik; Wengel, Jesper

doi:10.1002/(sici)1520-6866(1998)18:5<249::aid-tcm5>3.0.co;2-r

Cited by 15 publications

(1 citation statement)

References 24 publications

(29 reference statements)

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“…Slow cell death appears to occur at the G2/M transition and may involve events that normally occur at this stage of the cell cycle. Flow cytometry analysis revealed that the L-02 cells were arrested in the S and G2/M phases after AFB 1 exposure, indicating that the toxin induces cell cycle delay at the S phase and blockage at the G2 checkpoint, subsequently retarding cell division. , However, the coincubation with IgY alleviated this cell cycle arrest and significantly increased the percentage of G0/G1 cells in an IgY dose-dependent manner (Figure S5, P < 0.05). Therefore, IgY has a protective effect against AFB 1 -induced cell cycle arrest in hepatocytes, particularly at the G2/M stage.…”

Section: Resultsmentioning

confidence: 95%

IgY Reduces AFB₁-Induced Cytotoxicity, Cellular Dysfunction, and Genotoxicity in Human L-02 Hepatocytes and Swan 71 Trophoblasts

Qiu

Shen

Tian

et al. 2018

J. Agric. Food Chem.

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Aflatoxin B (AFB) causes hepatotoxic, genotoxic, and immunotoxic effects in a variety of species. Although various neutralizing agents of AFB toxicity have been studied, the egg yolk immunoglobulin (IgY) detoxification of small molecular toxins and the mechanisms underlying such effects have not yet been reported. In this investigation, anti-AFB IgY against AFB was successfully raised, and a competitive indirect enzyme-linked immunosorbent assay was established with a sensitive half-maximal inhibitory concentration (IC 2.4 ng/mL) and dynamic working range (0.13-43.0 ng/mL). The anti-AFB IgY obtained reduced AFB-induced cytotoxicity, cellular dysfunction, and genotoxicity by protecting cells against apoptotic body formation and DNA strand breaks, preventing G2/M phase cell cycle arrest, reducing AFB-DNA adduct and reactive oxygen species production and maintaining cell migration and invasion and the mitochondrial membrane potential. Anti-AFB IgY significantly inhibited the AFB-induced expression of proteins related to antioxidative, pro-apoptotic, and antiapoptotic processes in a strong dose-dependent manner. These experiments demonstrated that the anti-AFB IgY-bound AFB could not enter cells. This is the first time that IgY has been found to reduce the effects of small molecular toxins, which will be beneficial for the development of antibodies as detoxication agents.

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Section: Resultsmentioning

confidence: 95%

IgY Reduces AFB₁-Induced Cytotoxicity, Cellular Dysfunction, and Genotoxicity in Human L-02 Hepatocytes and Swan 71 Trophoblasts

Qiu

Shen

Tian

et al. 2018

J. Agric. Food Chem.

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Chemoprevention of benzene-induced bone marrow and pulmonary genotoxicity

Raj

Malik

Parmar

et al. 2001

Teratog. Carcinog. Mutagen.

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Our earlier studies documented the ability of 7,8-diacetoxy-4-methylcoumarin (DAMC) to cause irreversible inhibition of cytochrome P-450 linked mixed function oxidases (MFO) mediated by membrane bound DAMC: protein transacetylase. Since P-450 catalyzed oxidation of benzene is crucial to its toxic effects, the action of DAMC and related analogues were considered promising in preventing the genotoxicity due to benzene. For this purpose rats were pretreated with various acetoxy-4-methylcoumarins (test compounds), which was followed by the administration of benzene either intratracheally (IT) or intraperitoneally (IP), and sacrificed 26 h after the injection of benzene. The incidence of micronuclei (MN) in bone marrow (BM) and lung (LG) were assessed by light and fluorescent microscopy, respectively. A dose-dependent induction of MN in BM and LG cells was observed in rats administered with benzene. A significant reduction in benzene-induced MN in BM and LG was observed as a result of DAMC administration to rats; a higher dose of DAMC resulted in greater inhibition of clastogenic action of benzene as revealed by MN incidence. 7,8-dihydroxy-4-methylcoumarin (DHMC), the deacetylated product of DAMC, demonstrated relatively lesser potency to inhibit the clastogenic action of benzene. This observation is consistent with the ability of DAMC to inhibit the formation of benzene oxide as well as to scavenge the oxygen radicals formed during the course of benzene metabolism. The fact that DHMC can only scavenge the oxygen radicals and is ineffective in inhibiting benzene oxidation in vivo explains the reduced capability of dihydroxy coumarin to prevent MN due to benzene. 7-Acetoxy-4-methylcoumarin (MAC) inhibits the MN due to benzene being roughly 50% of that produced by DAMC. DAMC is also effective in normalizing the cell cycle alterations produced by benzene in BM and LG. These observations further substantiate our hypothesis that the biological effects of acetoxy coumarins are mediated by the action of membrane bound transacetylase that catalyzes the acetylation of concerned proteins. Teratogenesis Carcinog. Mutagen. 21:181-187, 2001.

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Probiotics and plant-derived compounds as eco-friendly agents to inhibit microbial toxins in poultry feed: a comprehensive review

El‐Hack

Samak

Noreldin

et al. 2018

Environ Sci Pollut Res

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Some of pathogenic bacteria and fungi have the ability to produce fetal toxins which may be the direct causes of cytotoxicity or cellular dysfunction in the colonization site. Biological and non-biological environmental factors, challenge and microbes influence the effect of toxins on these pathogens. Modern research mentions that many natural materials can reduce the production of toxins in pathogenic microbes. However, researches that explain the mechanical theories of their effects are meager. This review aimed to discuss the ameliorative potential role of plant-derived compounds and probiotics to reduce the toxin production of food-borne microbes either in poultry bodies or poultry feedstuff. Moreover, studies that highlight their own toxicological mechanisms have been discussed. Adding natural additives to feed has a clear positive effect on the enzymatic and microbiological appearance of the small intestine without any adverse effect on the liver. Studies in this respect were proposed to clarify the effects of these natural additives for feed. In conclusion, it could be suggested that the incorporation of probiotics, herbal extracts, and herbs in the poultry diets has some beneficial effects on productive performance, without a positive impact on economic efficiency. In addition, the use of these natural additives in feed has a useful impact on the microbiological appearance of the small intestine and do not have any adverse impacts on intestinal absorption or liver activity as evidenced by histological examination.

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Aflatoxin B1-induced micronuclei and cell cycle alterations in lung and bone marrow cells and their modulation byPiper argyrophyllumextract

Cited by 15 publications

References 24 publications

IgY Reduces AFB₁-Induced Cytotoxicity, Cellular Dysfunction, and Genotoxicity in Human L-02 Hepatocytes and Swan 71 Trophoblasts

IgY Reduces AFB₁-Induced Cytotoxicity, Cellular Dysfunction, and Genotoxicity in Human L-02 Hepatocytes and Swan 71 Trophoblasts

Chemoprevention of benzene-induced bone marrow and pulmonary genotoxicity

Probiotics and plant-derived compounds as eco-friendly agents to inhibit microbial toxins in poultry feed: a comprehensive review

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Aflatoxin B1-induced micronuclei and cell cycle alterations in lung and bone marrow cells and their modulation byPiper argyrophyllumextract

Cited by 15 publications

References 24 publications

IgY Reduces AFB1-Induced Cytotoxicity, Cellular Dysfunction, and Genotoxicity in Human L-02 Hepatocytes and Swan 71 Trophoblasts

IgY Reduces AFB1-Induced Cytotoxicity, Cellular Dysfunction, and Genotoxicity in Human L-02 Hepatocytes and Swan 71 Trophoblasts

Chemoprevention of benzene-induced bone marrow and pulmonary genotoxicity

Probiotics and plant-derived compounds as eco-friendly agents to inhibit microbial toxins in poultry feed: a comprehensive review

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Product

Resources

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IgY Reduces AFB₁-Induced Cytotoxicity, Cellular Dysfunction, and Genotoxicity in Human L-02 Hepatocytes and Swan 71 Trophoblasts

IgY Reduces AFB₁-Induced Cytotoxicity, Cellular Dysfunction, and Genotoxicity in Human L-02 Hepatocytes and Swan 71 Trophoblasts