Aeromonas hydrophila cellulitis. A case report

Porter, M-C; Murray-Leisure, Katherine A.; Dalbey, P

doi:10.7547/87507315-78-5-259

Cited by 2 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Separating these molecule species using molecular cloning coupled with Sanger sequencing is time-consuming and cost-inefficient [ 75 ]. Recent progress in bioinformatics tools (such as TIDE, Tracking of Indels by DEcomposition) enabled successful digital decoding of Sanger sequencing from a mixture of complex indels, generated by a unique CRISPR-targeting event into separate mutant species [ 76 – 78 ]. Although this method is still of limited sensitivity and remains to be validated on a larger scale, Sanger sequencing of a locally amplified, targeted locus offers a quick and reliable readout confirming the efficiency of any given CRISPR experiment.…”

Section: Crispr Efficiency Testmentioning

confidence: 99%

The Rise of CRISPR/Cas for Genome Editing in Stem Cells

Shui

Matias

Guo

et al. 2016

Stem Cells International

View full text Add to dashboard Cite

Genetic manipulation is a powerful tool to establish the causal relationship between a genetic lesion and a particular pathological phenotype. The rise of CRISPR/Cas9 genome-engineering tools overcame the traditional technical bottleneck for routine site-specific genetic manipulation in cells. To create the perfect in vitro cell model, there is significant interest from the stem cell research community to adopt this fast evolving technology. This review addresses this need directly by providing both the up-to-date biochemical rationale of CRISPR-mediated genome engineering and detailed practical guidelines for the design and execution of CRISPR experiments in cell models. Ultimately, this review will serve as a timely and comprehensive guide for this fast developing technology.

show abstract

Section: Crispr Efficiency Testmentioning

confidence: 99%