Aerobic composting of tobacco industry solid waste—simulation of the process

Briški, Felicita; Horgas, Nina; Vuković, Marija Brajdić; Gomzi, Zoran

doi:10.1007/s10098-003-0218-7

Cited by 57 publications

(44 citation statements)

References 7 publications

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“…icotine is an important environmental toxicant in tobacco and tobacco waste that endangers the health of humans (1)(2)(3). Several bacterial strains distributed in different genera have the ability to eliminate this toxic compound or transform it into commercially valuable compounds (4)(5)(6)(7).…”

mentioning

confidence: 99%

A Novel ( S )-6-Hydroxynicotine Oxidase Gene from Shinella sp. Strain HZN7

Qiu

Wei

et al. 2014

Appl Environ Microbiol

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Nicotine is an important environmental toxicant in tobacco waste. Shinella sp. strain HZN7 can metabolize nicotine into nontoxic compounds via variations of the pyridine and pyrrolidine pathways. However, the catabolic mechanism of this variant pathway at the gene or enzyme level is still unknown. In this study, two 6-hydroxynicotine degradation-deficient mutants, N7-M9 and N7-W3, were generated by transposon mutagenesis. The corresponding mutant genes, designated nctB and tnp2, were cloned and analyzed. The nctB gene encodes a novel flavin adenine dinucleotide-containing (S)-6-hydroxynicotine oxidase that converts (S)-6-hydroxynicotine into 6-hydroxy-N-methylmyosmine and then spontaneously hydrolyzes into 6-hydroxypseudooxynicotine. The deletion and complementation of the nctB gene showed that this enzyme is essential for nicotine or (S)-6-hydroxynicotine degradation. Purified NctB could also convert (S)-nicotine into N-methylmyosmine, which spontaneously hydrolyzed into pseudooxynicotine. The kinetic constants of NctB toward (S)-6-hydroxynicotine (K m ‫؍‬ 0.019 m⌴, k cat ‫؍‬ 7.3 s ؊1 ) and nicotine (K m ‫؍‬ 2.03 m⌴, k cat ‫؍‬ 0.396 s ؊1 ) indicated that (S)-6-hydroxynicotine is the preferred substrate in vivo. NctB showed no activities toward the R enantiomer of nicotine or 6-hydroxynicotine. Strain HZN7 could degrade (R)-nicotine into (R)-6-hydroxynicotine without any further degradation. The tnp2 gene from mutant N7-W3 encodes a putative transposase, and its deletion did not abolish the nicotine degradation activity. This study advances the understanding of the microbial diversity of nicotine biodegradation.

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confidence: 99%

A Novel ( S )-6-Hydroxynicotine Oxidase Gene from Shinella sp. Strain HZN7

Qiu

Wei

et al. 2014

Appl Environ Microbiol

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“…Large quantities of tobacco waste containing high concentrations of nicotine are generated annually by tobacco manufacturing and activities that use tobacco (Briški et al 2003). As one of the most harmful components of tobacco waste, nicotine is an environmental concern because it is extremely toxic to most organisms and could cause ecological problems (Civilini et al 1997;American Cancer Society 2005).…”

Section: Introductionmentioning

confidence: 99%

Nicotine degradation by two novel bacterial isolates of Acinetobacter sp. TW and Sphingomonas sp. TY and their responses in the presence of neonicotinoid insecticides

Wang

Yang

Wang

et al. 2010

World J Microbiol Biotechnol

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Two novel nicotine-degrading bacterial strains were isolated from tobacco waste and identified as Acinetobacter sp. TW and Sphingomonas sp. TY based on morphology, physiological and biochemical tests, Biolog analysis and 16S rDNA sequencing. The 16S rDNA sequences have been deposited in GenBank under the accession numbers FJ753401 for TW and FJ754274 for TY. The best culture conditions for nicotine degradation were 25-37°C and pH 7.0-8.0 for strain TW and 25-30°C and pH 6.0-7.0 for strain TY. Under the best conditions, the cell growth and nicotine-degradation kinetics of the two isolates were assessed, and 1.0 g/l nicotine was completely degraded within 12 and 18 h for TW and TY, respectively. Moreover, the presence of four widely-used commercial neonicotinoid insecticides in the medium had no effects on nicotine degradation by TW; among the four tested neonicotinoids, only thiamethoxam significantly delayed nicotine degradation by TY. TW and TY were also able to degrade selected neonicotinoids. This is the first report of nicotine degradation by Acinetobacter sp. and Sphingomonas sp. This study showed that these two newly isolated bacteria may be suitable for the disposal of tobacco waste and the reduction of nicotine in tobacco leaves.

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“…A large quantity of tobacco waste with a high nicotine concentration is produced during tobacco processing, and has become a serious ecological and environmental problem (Civilini et al 1997;Briški et al 2003). SBR technology is an attractive alternative for dealing with pollutant wastewater due to its flexibility and efficiency (Celis et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Reduction of oxidative stress by bioaugmented strain Pseudomonas sp. HF-1 and selection of potential biomarkers in sequencing batch reactor treating tobacco wastewater

et al. 2010

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Oxidative stress induced by toxic pollutants is generally responsible for the poor performance of many sequencing batch reactors (SBRs) treating organic wastewater. In this study, the oxidative stress in two SBR systems that dealt with tobacco wastewater was monitored by measuring four popular biomarkers (superoxide dismutase, SOD; catalase, CAT; glutathione, GSH; and malondialdehyde, MDA). In the non-BA (non-bio-augmented) system, more intense oxidative stress was induced by a higher concentration of nicotine in tobacco wastewater, and excessive oxidative stress was induced by 250 mg/l of nicotine at the final stage. However, when a nicotine-degrading bacterial strain Pseudomonas sp. HF-1 was added to the BA (bio-augmented) system, the oxidative stress was significantly reduced compared to the non-BA system (p < 0.01).These results suggested that the oxidative stress was mainly induced by nicotine in the SBR treatment of tobacco wastewater, and that bioaugmentation with strain HF-1 would be a potential strategy to reduce the oxidative stress and thereby improve the performance in SBRs. Additionally, the positive correlation between the nicotine content and CAT, GSH and MDA activity in both systems implied that these parameters can be used as biomarkers for reflecting the performance of SBR treatment of tobacco wastewater, and in monitoring nicotine environmental pollution.

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Aerobic composting of tobacco industry solid waste—simulation of the process

Cited by 57 publications

References 7 publications

A Novel ( S )-6-Hydroxynicotine Oxidase Gene from Shinella sp. Strain HZN7

A Novel ( S )-6-Hydroxynicotine Oxidase Gene from Shinella sp. Strain HZN7

Nicotine degradation by two novel bacterial isolates of Acinetobacter sp. TW and Sphingomonas sp. TY and their responses in the presence of neonicotinoid insecticides

Reduction of oxidative stress by bioaugmented strain Pseudomonas sp. HF-1 and selection of potential biomarkers in sequencing batch reactor treating tobacco wastewater

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