Adventitious Shoot Regeneration from In Vitro Leaf Explants of the Peach Rootstock Hansen 536

Ricci, A.; Capriotti, Luca; Mezzetti, Bruno; Navacchi, O.; Sabbadini, Silvia

doi:10.3390/plants9060755

Cited by 15 publications

(7 citation statements)

References 56 publications

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“…However, in this instance, greater regeneration could have been achieved with the abaxial orientation, as it would provide a larger surface area of the explant in contact with the medium. This is because when the adaxial side of the leaf is positioned in contact with the medium, it tends to roll up at both ends (Ricci et al 2020). 'Legacy' leaves in the abaxial position had the best responses among explants, achieving the highest number of shoots per explant (8.4) with 1mg/L ZT (Fig.…”

Section: Shoot Regenerationmentioning

confidence: 99%

Autopolyploidization and in vitro regeneration of three blueberry cultivars from leaves and microstems.

Tauler,

Martínez-Barradas,

Romero-Romero

et al. 2024

Preprint

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Blueberries are a fruit with an increasing global demand due to their phytochemical and bioactive compounds content. They are promoted worldwide because of their health benefits. For optimal growth and productivity, blueberry crops need acidic soil pH, specific chilling hours, and an adequate atmospheric temperature. This delicate production equilibrium is under severe threat from climate change, potentially leading to reduced yields and increased cultivation costs unless new cultivars are developed for each edafoclimatic zone. Therefore, considering varietal replacements with more productive cultivars offering higher quality and better adaptability to local conditions is imperative. In this study, we employ polyploidization and in vitro tissue culture to promote variability and lay the foundation for new cultivar development. We report the successful induction of octoploids in three blueberry cultivars, namely 'Biloxi,' 'Legacy,' and 'Duke', through whole-genome duplication. Leaves and microstem explants were exposed to 0.1% colchicine for 24 and 48 hours in in vitro culture. After analyzing the polyploid level of 160 regenerated shoots using DNA flow cytometry, we obtained a total of 18 mutants, consisting of 8 mixoploids and 10 octoploids. The number of chloroplasts in the stomata was analysed by fluorescence microscopy, revealing the duplication of these organelles in the induced octoploid plants. To our knowledge, this represents the first successful induction of octoploids in three blueberry cultivars -'Biloxi,' 'Legacy,' and 'Duke'- achieved by exposing leaves and microstem explants to colchicine in in vitro culture. This technique holds promise as a valuable tool for the development of improved blueberry cultivars.

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Section: Shoot Regenerationmentioning

confidence: 99%

Autopolyploidization and in vitro regeneration of three blueberry cultivars from leaves and microstems.

Tauler,

Martínez-Barradas,

Romero-Romero

et al. 2024

Preprint

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“…Differences in response to organogenesis depend on the type of explant and different PGRs combinations. The basal media enriched with cytokinin BA with combinations of IBA or NAA shows the highest regeneration frequencies (Sabbadini et al, 2019;Ricci et al, 2020). BA has a positive influence on peach shoot regeneration (Gentile et al, 2002;Pérez-Jiménez et al, 2012;Pérez-Jiménez et al, 2013;Sabbadini et al, 2019;Ricci et al, 2020).…”

Section: Peach Regenerationmentioning

confidence: 99%

“…The basal media enriched with cytokinin BA with combinations of IBA or NAA shows the highest regeneration frequencies (Sabbadini et al, 2019;Ricci et al, 2020). BA has a positive influence on peach shoot regeneration (Gentile et al, 2002;Pérez-Jiménez et al, 2012;Pérez-Jiménez et al, 2013;Sabbadini et al, 2019;Ricci et al, 2020). The addition of BA causes callus induction and elongation of the axis in peach using embryos as explant (Bhansali et al, 1990) and increase the number of shoot per explant in Boechera oleracea (Cheng et al, 2001).…”

Section: Peach Regenerationmentioning

confidence: 99%

“…However, these techniques are difficult and consuming time. Instead of these techniques, an adventitious shoot regeneration system was applied in peach regeneration using different explants: leaves (Ricci et al, 2020); mature embryos (Pérez-Clemente et al, 2004); immature embryos (Hammerschlag et al, 1985); cotyledons of mature seed explant (Abdelsattar et al, 2020), meristematic bulks (Sabbadini et al, 2019) and flower, petiole and stem calyx (Pérez-Jiménez et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Expression of some Epigenetic-Related Genes in Regenerated Shoots of Prunus persica

Ismail

Ali

Nagaty

et al. 2020

Journal of Agricultural Chemistry and Biotechnology

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Establishment of an efficient adventitious shoot regeneration system for mature embryo of Prunus persica via shoot organogenesis has been achieved. Three different combinations of plant growth regulators (PGRs) have been applied in the current study; 6-Benzyl adenine (BA) or thidiazuron (TDZ) with Indole acetic acid (IAA) or Indole butyric acid (IBA). The high regeneration frequency was illustrated by 1.5 mg/l BA with 0.5 mg/l IAA combinations. Quantitative expression of eight epigenetic regulatory genes; Chromomethylase 3 (CMT3), Domains Rearranged Methyltransferase2 (DRM2), Fertilization Independent Endosperm (FIE2) and Chromodomain Protein Like Heterochromatin Protein1 (LHP1), Pickle (PKL), Proliferating Cell Nuclear Antigen (PCNA), Shoot Meristemless (STM) and DICER-LIKE1 (DCL1) have been estimated in the peach regenerated shoots. We found that the auxins and cytokinins in different combinations especially BA effect on the expression of some epigenetic genes in relation to the mature embryo.

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“…Under a humid environment, STS reduces the emission of carnation fragrance after harvesting, thus prolonging the fragrance of carnations ( Kishimoto, 2021 ). Supplementation of the culture medium with STS significantly increases the number of regenerating shoots per leaf and significantly improves the in vitro regeneration frequency of a peach rootstock ( Ricci et al., 2020 ). Although STS solution effectively prolongs the vase life of rose cut flowers ( Son et al., 2003 ), the detailed mechanism by which STS delays petal abscission in rose remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Metabolome and transcriptome integration reveals insights into the process of delayed petal abscission in rose by STS

Zhang

et al. 2022

Front. Plant Sci.

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The abscission of plant organs plays an important role in ensuring the normal life activities. Rose is one of the most important ornamental plants, and its premature abscission of petal has seriously affected the quality and commercial value. Silver Thiosulfate (STS) is an ethylene inhibitor, which is often used preservative to delay the senescence of fresh cut flowers. To understand the regulatory mechanism of petal abscission in rose by STS, integrative analysis of the metabolome and transcriptome profiles was performed in abscission zone (AZ) tissues of rose under different treatments (MOCK, STS, ETH, STS+ETH). The results showed that STS significantly delayed the petal abscission in phenotype and reduced the activity of two enzymes (pectinase and cellulase) associated with cell wall degradation in physiological level. STS affected the contents of five metabolites (shikonin, jasmonic acid, gluconolactone, stachyose and D-Erythrose 4-phosphate), and involved changes in the expression of 39 differentially expressed genes (DEGs) associated with these five metabolites. Five DEGs (LOC112192149, LOC112196726, LOC112189737, LOC112188495, and LOC112188936) were probably directly associated with the biosynthesis of shikonin, jasmonic acid, and D-Erythrose 4-phosphate. Meanwhile, the effect of STS on the abscission process significantly involved in the pentose phosphate pathway and amino acid biosynthesis pathway. In addition, STS had a greater effect on the transcription factors, phytohormone related DEGs represented by auxin and ethylene, DEGs related to disease resistance and amino acid, etc. Above all, STS negatively influences petal abscission of rose, these results maybe provide a reference for subsequent studies on petal abscission of rose by STS.

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Adventitious Shoot Regeneration from In Vitro Leaf Explants of the Peach Rootstock Hansen 536

Cited by 15 publications

References 56 publications

Autopolyploidization and in vitro regeneration of three blueberry cultivars from leaves and microstems.

Autopolyploidization and in vitro regeneration of three blueberry cultivars from leaves and microstems.

Expression of some Epigenetic-Related Genes in Regenerated Shoots of Prunus persica

Metabolome and transcriptome integration reveals insights into the process of delayed petal abscission in rose by STS

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