Metabolome and transcriptome integration reveals insights into the process of delayed petal abscission in rose by STS

Background During Fritillaria thunbergii planting, pests and diseases usually invade the plant, resulting in reduced yield and quality. Previous studies have demonstrated that using biocontrol agents can effectively control grubs and affect the steroid alkaloids content in F. thunbergii. However, the molecular regulatory mechanisms underlying the differences in the accumulation of steroid alkaloids in response to biocontrol agents remain unclear. Results Combined transcriptomic and metabolic analyses were performed by treating the bulbs of F. thunbergii treated with biocontrol agents during planting. Otherwise, 48 alkaloids including 32 steroid alkaloids, 6 indole alkaloids, 2 scopolamine-type alkaloids, 1 isoquinoline alkaloid, 1 furoquinoline alkaloid, and 6 other alkaloids were identified. The content of steroidal alkaloids particularly peimine, peiminine, and veratramine, increased significantly in the group treated with the biocontrol agents. Transcriptome sequencing identified 929 differential genes using biocontrol agents, including 589 upregulated and 340 downregulated genes. Putative biosynthesis networks of steroid alkaloids have been established and combined with differentially expressed structural unigenes, such as acetyl-CoA C-acetyl-transferase, acelyl-CoAC-acetyltransferase3-hydroxy-3-methylglutaryl-coenzyme A synthase, 1-deoxy-D-xylulose-5-phosphate reductor-isomerase, 2-C-methyl-D-erythritol-4-phosphate cytidylyltransferase and 4-hydroxy-3-methylbut-2-enyl diphosphate reductase. In addition, biological processes such as amino acid accumulation and oxidative phosphorylation were predicted to be related to the synthesis of steroid alkaloids. Cytochrome P450 enzymes also play crucial roles in the steroid alkaloid synthesis. The transcription factor families MYB and bHLH were significantly upregulated after using biocontrol agents. Conclusions Biocontrol agents increased the steroid alkaloids accumulation of steroid alkaloids by affecting key enzymes in the steroid alkaloid synthesis pathway, biological processes of oxidative phosphorylation and amino acid synthesis, cytochrome P450 enzymes, and transcription factors. This study revealed the mechanism underlying the difference in steroidal alkaloids in F. thunbergii after using biocontrol agents, laying the groundwork for future industrial production of steroid alkaloids and ecological planting of medicinal materials in the future.

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Eucommia ulmoides leaf extract attenuates cisplatin-induced kidney injury in mice through endoplasmic reticulum stress and biometabolic mechanism

Lin,

Xiong,

Zhang

et al. 2024

Preprint

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Cisplatin (CP) is a widely utilized anticancer drug but is associated with significant side effects, notably acute kidney injury (AKI). Eucommia ulmoides leaf (EUL), a valuable Chinese herbal remedy, is known for its renoprotective properties. However, the function and underlying pathways of EUL in AKI therapy have remained largely unexplored. This research aimed to elucidate the protective roles of EUL in an AKI mouse model through biochemical assays, and histopathological andexaminations while also investigating the underlying mechanisms via endoplasmic reticulum (ER) stress-related protein expression analysis and metabolomics. The findings demonstrated that pretreatment with orally administered EUL significantly reduced blood urea nitrogen (BUN) and serum creatinine (SCr) levels, ameliorated CP-induced kidney histopathological injuries, and attenuated CP-induced ER stress by reducing the protein expressions of PERK, IRE 1α, GRP78, ATF6, ATF4, and CHOP. Additionally, metabolomics analysis identified 31 significant differential metabolites affected by EUL treatment in AKI mice, impacting pathways related to taurine and hypotaurine metabolism, lysine degradation, and steroid hormone biosynthesis. These findings suggested that EUL could offer valuable insights for potential CP-induced AKI treatment strategies.

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Metabolome and transcriptome integration reveals insights into the process of delayed petal abscission in rose by STS

Cited by 3 publications

References 47 publications

Advances in understanding the mechanisms of organ abscission in vivo and in vitro plants

Advances in understanding the mechanisms of organ abscission in vivo and in vitro plants

Metabolite profiling and transcriptomic analyses demonstrate the effects of biocontrol agents on alkaloid accumulation in Fritillaria thunbergii

Eucommia ulmoides leaf extract attenuates cisplatin-induced kidney injury in mice through endoplasmic reticulum stress and biometabolic mechanism

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