Advantages Afforded by Miniaturization and Integration of DNA Analysis Instrumentation

Northrup, M. Allen; Benett, Bill; Hadley, Dean R.; Stratton, Paul; Landre, Phoebe

doi:10.1007/978-3-642-72076-5_33

Cited by 3 publications

(3 citation statements)

References 12 publications

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“…They are being developed in order to minimize the process time as well as power consumption and required space. [4][5][6][7] In addition, microsystem technology opens the way to miniaturized complete DNA analysis systems. 8,9 Chip thermocyclers are realized in several types in the laboratory.…”

Section: Introductionmentioning

confidence: 99%

Miniaturized flow-through PCR with different template types in a silicon chip thermocycler

Bräutigam²,

2001

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Flow-through chip thermocyclers can be used in miniaturized rapid polymerase chain reaction (PCR) despite their high surface to volume ratio of samples. We demonstrated that a thermocycler made of silicon and glass chips and containing thin film transducers for heating and temperature control can be adapted to the amplification of various DNA templates of different sources and properties. Therefore, the concept of serial flow in a liquid/liquid two-phase system was combined with a surface management of inner side walls of the microchannel and an adaptation of PCR mixture composition. In addition, the process temperatures and the flow rates were optimized. Thus, a synthetic template originating from investigations on nucleic acid evolution with 106 base pairs [cooperative amplification of templates by cross hybridization (CATCH)], a house keeping gene with 379 base pairs [glutaraldehyde 3-phosphate dehydrogenase (GAPDH)] and a zinc finger protein relevant in human pathogenesis with 700 base pairs [Myc-interacting zinc finger protein-1, knock-out (Miz1-KO)] were amplified successfully. In all three cases the selectivity of priming and amplification could be shown by gel electrophoresis. The typical amplification time was 1 min per temperature cycle. So, the typical residence time of a sample volume inside the 25 cycle device amounts to less then half an hour. The energy consumption of the PCR chip for a 35 min PCR process amounts to less than 0.012 kW h.

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Section: Introductionmentioning

confidence: 99%

Miniaturized flow-through PCR with different template types in a silicon chip thermocycler

Bräutigam²,

2001

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“…This kind of microfabrication is a useful method to fabricate the miniaturized devices. Some reports have demonstrated the general advantages of miniaturized systems, compared to conventional large synthesis devices [19][20][21][22][23]. The miniaturization of these systems has many merits, such as the small volume reagents/sample consumption, reliability, automatic performance control, and portability at the point of care, which are beneficial to the bio/chemical analysis.…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive and Miniaturized Fluorescence Detection System with an Autonomous Capillary Fluid Manipulation Chip

2012

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This paper presents a novel, highly sensitive and ultra-small fluorescent detection system, including an autonomous capillary fluid manipulation chip. The optical detector integrates a LED light source, all necessary optical components, and a photodiode with preamplifier into one package of about 2 cm × 2 cm × 2 cm. Also, the low-cost and simple pumpless microfluidic device works well in sample preparation and manipulation. This chip consists of capillary stop valves and trigger valves which are fabricated by lithography and then bonded with a polydimethylsiloxane-ethylene oxide polymer polydimethylsiloxane (PEO-PDMS) cover. The contact angle of the PEO-PDMS can be adjusted by changing the concentration of the PEO. Hence, the fluidic chip can achieve functionalities such as timing features and basic logical functions. The prototype has been tested by fluorescence dye 5-Carboxyfluorescein (5-FAM) dissolved into the solvent DMSO (Dimethyl Sulfoxide). The results prove a remarkable sensitivity at a pico-scale molar, around 1.08 pM. The low-cost and miniaturized optical detection system, with a self-control capillary-driven microfluidic chip developed in this work, can be used as the crucial parts in portable biochemical detection applications and point of care testing.

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“…As reported in some excellent reviews, these include integrated chip-based separation methods, − biochemical application of microsystems, and the clinical potential of chip-based analysis systems . Over the past decade, this trend has been driven by the need of rapid, on-line measurements at low concentration in the fields of chemical synthesis, DNA sequencing, drug discovery, pharmaceutical screening, medical diagnostic, and environmental analysis. − A large number of applications clearly demonstrate fundamental advantages of miniaturized analysis systems, as compared to lab-scale instrumentation. − …”

mentioning

confidence: 99%

Integrated Microfluidic System Enabling (Bio)chemical Reactions with On-Line MALDI-TOF Mass Spectrometry

Brivio

Fokkens²,

Verboom³

et al. 2002

Anal. Chem.

105

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A continuous flow micro total analysis system (micro-TAS) consisting of an on-chip microfluidic device connected to a matrix assisted laser desorption ionization [MALDI] time-of-flight [TOF] mass spectrometer (MS) as an analytical screening system is presented. Reaction microchannels and inlet/outlet reservoirs were fabricated by powderblasting on glass wafers that were then bonded to silicon substrates. The novel lab-on-a-chip was realized by integrating the microdevice with a MALDI-TOFMS standard sample plate used as carrier to get the microfluidic device in the MALDI instrument. A novel pressure-driven pumping mechanism using the vacuum of the instrument as a driving force induces flow in the reaction microchannel in a self-activating way. Organic syntheses as well as biochemical reactions are carried out entirely inside the MALDI-MS ionization vacuum chamber and analyzed on-line by MALDI-TOFMS in real time. The effectiveness of the micro-TAS system has been successfully demonstrated with several examples of (bio)chemical reactions.

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Advantages Afforded by Miniaturization and Integration of DNA Analysis Instrumentation

Cited by 3 publications

References 12 publications

Miniaturized flow-through PCR with different template types in a silicon chip thermocycler

Miniaturized flow-through PCR with different template types in a silicon chip thermocycler

Highly Sensitive and Miniaturized Fluorescence Detection System with an Autonomous Capillary Fluid Manipulation Chip

Integrated Microfluidic System Enabling (Bio)chemical Reactions with On-Line MALDI-TOF Mass Spectrometry

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