Advancing quality control of food samples by Next Generation Sequencing compared to culture-dependent techniques

Dimitrakopoulou, Maria-Eleni; Kotsalou, Chrysoula; Stavrou, Venia; Vantarakis, Apostolos

doi:10.26502/jfsnr.2642-11000066

Cited by 1 publication

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PCR conditions were subjected to initial denaturation at 94°C for 2 min, followed by 35 cycles of denaturation at 94°C for 1 min, annealing at 45°C for 40 s, elongation at 72°C for 2 min, and final extension at 72°C for 10 min. PCR products were separated by electrophoresis 2% agarose gel and 100 bp DNA ladder (Lonza) was used as DNA molecular weight marker 31 …”

Section: Methodsmentioning

confidence: 99%

“…[28][29][30] In our knowledge, there is a study regarding quality control of Avgotaracho Mesolonghiou and Vostizza currant, by means of Next Generation Sequencing. 31 Although, this is the first time, that these Greek PDO products analyzed by DNA-based methods in terms of authenticity and traceability.…”

Section: Introductionmentioning

confidence: 90%

“…Moreover, currants considered to provide beneficial health effects on digestive system, on bones (preventing osteoporosis and arthritis), on insulin levels and on cardiovascular and neurodegenerative diseases 28–30 . In our knowledge, there is a study regarding quality control of Avgotaracho Mesolonghiou and Vostizza currant, by means of Next Generation Sequencing 31 . Although, this is the first time, that these Greek PDO products analyzed by DNA‐based methods in terms of authenticity and traceability.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Potential biological markers by DNA‐based tools for determination of Greek PDO geographical origin and authenticity: “Avgotaracho Mesolonghiou” and “Vostizza currant”

et al. 2021

Self Cite

View full text Add to dashboard Cite

Background: Food traceability and authentication had become mandatory for food industry and global food trade. Numerous DNA-based methods could contribute against food frauds, because of their advantages such as simplicity, accuracy, and robustness.The aim of this study was to explore whether unique biological markers for two high valuable and popular Greek protected designation of origin (PDO) products could be indicated. For this purpose, "Avgotaracho Mesolonghiou" known as Greek caviar and "Vostizza" currant were subjected to DNA-based analysis. PCR-RAPD, PCR-RFLP, and PCR-DGGE were performed for Greek PDO products and potential biological markers were explored, based on either genomic DNA or bacteria communities.Results: Band profiles resulted in molecular techniques, could be used as a "barcode" to certify the origin and authenticity of PDO products. Conclusion:These methods are proposed, as alternative traceability tools, in order to provide unique markers and could be the key to "farm to table" challenge. Therefore, biological markers could be used throughout the entire commercial supply chain and protect food products, which hold a quality scheme, from adulterations.

show abstract

Section: Methodsmentioning

confidence: 99%