Advances in sample preparation and analytical techniques for lipidomics study of clinical samples

Teo, Chin Chye; Chong, William P. K.; E, Tan; Basri, Nurhidayah; Low, Zhen Jie; Ho, Ying Swan

doi:10.1016/j.trac.2014.10.010

Cited by 74 publications

(63 citation statements)

References 146 publications

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“…Plasma and serum samples are the most effected by PL interference in analysis, owing to the possibility of approximately 17,000 different types of lipids and fatty acids being present (Teo et al, 2015). The number and type of phospholipids present in these samples can vary widely, depending on the species, metabolism and diet of the animal from which the sample was obtained (Gao et al, 2006;Shen et al, 2005).…”

Section: What Is the Impact Of Phospholipids On Bioanalytical Methods?mentioning

confidence: 99%

“…The most common solvents used in LLE are mixtures of chloroform, methanol and water (in differing ratios), but chloroform is often replaced with dichloromethane now owing to its lesser toxicity, with similar results (Reis et al, 2013). Modifications of LLE solvent include using additives such as acetic acid, hydrochloric acid, formic acid, citric acid, ethyl acetate and n-butyl chloride, as well as replacing chloroform with MTBE or butanol (Bylda et al, 2014;Jemal et al, 2010;Teo et al, 2015). Most of these modifications are specific to the analyte in question or are for the purpose of combatting matrix effects from a particular species of PL, further supporting the need to individualize the solvent and system used (Michopoulos et al, 2011).…”

Section: Methods Of Pl Removal Prior To Analysismentioning

confidence: 99%

“…Phospholipids are a primary component of cell membranes, and are found in varying levels in many biological fluids, including plasma, urine, tears, cerebrospinal fluid and synovial fluid, and in tissues (Teo et al, 2015). Analysis of biological samples for the purpose of determination of drugs, metabolites and other xenobiotics is often hindered by the presence of these phospholipids, especially when using HPLC coupled with mass spectrometry (MS) (Furey et al, 2013;Kushon and Pike, 2012;Rossmann et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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The impact of phospholipids and phospholipid removal on bioanalytical method performance

Carmical

Brown

2016

Biomedical Chromatography

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Phospholipids (PLs) are a component of cellmembranes, biological fluids and tissues. These compounds are problematic for the bioanalytical chemist, especially when PLs are not the analytes of interest. PL interference with bioanalysis highly impacts reverse-phase chromatographic methods coupled with mass spectrometric detection. Phospholipids are strongly retained on hydrophobic columns, and can cause significant ionization suppression in the mass spectrometer, as they outcompete analyte molecules for ionization. Strategies for improving analyte detection in the presence of PLs are reviewed, including in-analysis modifications and sample preparation strategies. Removal of interfering PLs prior to analysis seems to be most effective atmoderating thematrix effects fromthese endogenous cellular components, and has the potential to simplify chromatography and improve column lifetime. Products targeted at PL removal for sample pre-treatment, as well as products that combine multiplemodes of sample preparation (i.e. Hybrid SPE), show significant promise inmediating the effect on PL interference in bioanalysis.

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Section: What Is the Impact Of Phospholipids On Bioanalytical Methods?mentioning

confidence: 99%

Section: Methods Of Pl Removal Prior To Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The impact of phospholipids and phospholipid removal on bioanalytical method performance

Carmical

Brown

2016

Biomedical Chromatography

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“…using chloroform/methanol as the extraction solvent, which was improved later by Bligh and Dyer . Besides, many other extraction methods of lipids have been developed in past years, such as SPE and MAE , which have been summarized in other reviews .…”

Section: Analytical Methods In Clinical Lipidomicsmentioning

confidence: 99%

Recent advances in lipidomics for disease research

Yang

Shan

et al. 2015

J of Separation Science

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Lipidomics is an important branch of metabolomics, which aims at the detailed analysis of lipid species and their multiple roles in the living system. In recent years, the development of various analytical methods for effective identification and characterization of lipids has greatly promoted the process of lipidomics. Meanwhile, as many diseases demonstrate a remarkable alteration in lipid profiles compared with that of healthy people, lipidomics has been extensively introduced to disease research. The comprehensive lipid profiling provides a chance to discover novel biomarkers for specific disease. In addition, it plays a crucial role in the study of lipid metabolism, which could illuminate the pathogenesis of diseases. In this review, after brief discussion of analytical methods for lipidomics in clinical research, we focus on the recent advances of lipidomics related to four types of diseases, including cancer, atherosclerosis, diabetes mellitus, and Alzheimer's disease.

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“…Trying to achieve these objectives, while avoiding the inherent problems of the two-phase extraction methods, one-phase lipid extractions have recently been developed [2, 3, 15, 16]. One-phase extractions focus on an “all-in-one-tube” approach eliminating the need for phase separation by denaturing proteins that are later removed by centrifugation.…”

Section: Introductionmentioning

confidence: 99%

One- vs two-phase extraction: re-evaluation of sample preparation procedures for untargeted lipidomics in plasma samples

et al. 2018

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Lipidomics is a rapidly developing field in modern biomedical research. While LC-MS systems are able to detect most of the known lipid classes in a biological matrix, there is no single technique able to extract all of them simultaneously. In comparison with two-phase extractions, one-phase extraction systems are of particular interest, since they decrease the complexity of the experimental procedure. By using an untargeted lipidomics approach, we explored the differences/similarities between the most commonly used two-phase extraction systems (Folch, Bligh and Dyer, and MTBE) and one of the more recently introduced one-phase extraction systems for lipid analysis based on the MMC solvent mixture (MeOH/MTBE/CHCl3). The four extraction methods were evaluated and thoroughly compared against a pooled extract that qualitatively and quantitatively represents the average of the combined extractions. Our results show that the lipid profile obtained with the MMC system displayed the highest similarity to the pooled extract, indicating that it was most representative of the lipidome in the original sample. Furthermore, it showed better extraction efficiencies for moderate and highly apolar lipid species in comparison with the Folch, Bligh and Dyer, and MTBE extraction systems. Finally, the technical simplicity of the MMC procedure makes this solvent system highly suitable for automated, untargeted lipidomics analysis.Electronic supplementary materialThe online version of this article (10.1007/s00216-018-1200-x) contains supplementary material, which is available to authorized users.

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Advances in sample preparation and analytical techniques for lipidomics study of clinical samples

Cited by 74 publications

References 146 publications

The impact of phospholipids and phospholipid removal on bioanalytical method performance

The impact of phospholipids and phospholipid removal on bioanalytical method performance

Recent advances in lipidomics for disease research

One- vs two-phase extraction: re-evaluation of sample preparation procedures for untargeted lipidomics in plasma samples

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