“…Real-time PCR is characterized by a much higher sensitivity compared to conventional PCR, being able to detect even very low copies of DNA (Angelone-Alasaad et al, 2015). For this reason, this modern method is now commonly used to amplify small or degraded samples (Holt et al, 2016;Lee, McCord, & Buel, 2014) or for diagnostic purposes (Caraguel, Stryhn, Gagne′, Dohoo, & Hammell, 2011). Although we only tested our method on fresh or well-preserved samples, given its sensitivity, it is likely to work on degraded (e.g., museum specimen) or low-DNA samples obtained through noninvasive sampling, such as feces and hair (e.g., obtained through hair-tubes; Kanthaswamy, Premasuthan, Ng, Satkoski, & Goyal, 2012).…”