Advances in aptamer screening technologies

Yan, Jun; Xiong, Hongjie; Cai, Shundong; Wen, Nachuan; He, Qunye; Liu, Yanfei; Peng, Dongming; Liu, Zhenbao

doi:10.1016/j.talanta.2019.03.015

Cited by 93 publications

(48 citation statements)

References 179 publications

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“…Screened from a large pool of random ssDNA sequences in vitro , ATP aptamers are single strand DNA (ssDNA) with high binding affinity against ATP [132] , [133] , [134] . ATP aptamers can bind with single strand nucleotides forming DNA duplex via complementary base pairing.…”

Section: Stimuli Triggered Drug Deliverymentioning

confidence: 99%

Tumor microenvironment responsive drug delivery systems

Chen

Yan

et al. 2020

Asian Journal of Pharmaceutical Sciences

Self Cite

125

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Conventional tumor-targeted drug delivery systems (DDSs) face challenges, such as unsatisfied systemic circulation, low targeting efficiency, poor tumoral penetration, and uncontrolled drug release. Recently, tumor cellular molecules-triggered DDSs have aroused great interests in addressing such dilemmas. With the introduction of several additional functionalities, the properties of these smart DDSs including size, surface charge and ligand exposure can response to different tumor microenvironments for a more efficient tumor targeting, and eventually achieve desired drug release for an optimized therapeutic efficiency. This review highlights the recent research progresses on smart tumor environment responsive drug delivery systems for targeted drug delivery. Dynamic targeting strategies and functional moieties sensitive to a variety of tumor cellular stimuli, including pH, glutathione, adenosine-triphosphate, reactive oxygen species, enzyme and inflammatory factors are summarized. Special emphasis of this review is placed on their responsive mechanisms, drug loading models, drawbacks and merits. Several typical multi-stimuli responsive DDSs are listed. And the main challenges and potential future development are discussed.

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Section: Stimuli Triggered Drug Deliverymentioning

confidence: 99%

Tumor microenvironment responsive drug delivery systems

Chen

Yan

et al. 2020

Asian Journal of Pharmaceutical Sciences

Self Cite

125

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“…However, when bacterial or viral targets bear significant negative charges, it will enhance the difficulty to enrich highly-specific aptamer candidates through an SELEX process [ 47 ], because oligonucleotide-based aptamers have negative charges on their backbone [ 48 ]. To obtain highly-specific aptamer candidates, the stringency of a SELEX selection process is critical, and negative and countermeasures may be often introduced to exclude low affinity or non-specific aptamers [ 49 ]. At the same time, an introduction of additional counter steps might have a negative impact by increasing the time and cost for an overall selection process [ 7 ].…”

Section: Perspectives and Conclusionmentioning

confidence: 99%

Advances in detection of infectious agents by aptamer-based technologies

Jia

et al. 2020

Emerging Microbes & Infections

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Infectious diseases still remain one of the biggest challenges for human health. Accurate and early detection of infectious pathogens are crucial for transmission control, clinical diagnosis, and therapy. For a traditional reason, most immunological and microbiological laboratories are equipped with instruments designated for antibody-based assays in detection of infectious pathogens or clinical diagnosis. Emerging aptamer-based technologies have pushed a shift from antibody-based to aptamer-based assays due to equal specificity, even better sensitivity, lower manufacturing cost and more flexibility in amending for chemiluminescent, electrochemical or fluorescent detection in a multifaceted and high throughput fashion in comparison of aptamer-based to antibody-based assays. The nature of aptamer-based technologies is particularly suitable for point-of-care testing in remote areas at warm or hot atmosphere, and mass screening for potential infection in pandemic of emerging infectious agents, such as SARS-CoV or SARS-CoV-2 in an epicentre or other regions. This review intends to summarize currently available aptamer-based technologies in detection of bacterial, viral, and protozoan pathogens for research and clinical application. It is anticipated that potential technologies will be further optimized and validated for clinical translation in meeting increasing demands for prompt, precise, and reliable detection of specific pathogens in various atmospheric conditions.

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“…Aptamers are a kind of short‐chain nucleotide that can recognize target allergens. Aptamers can fold into various stable secondary structures (stem, bugle, loop, and G‑quadruplex) and form unique three‐dimensional structures with target allergens through the pairing of complementary bases (Yan et al., 2019) and Electrostatic and hydrogen bonding. Currently, aptamers attracted a great attention and several aptasensing detection strategies have been investigated for food allergen detection because of their small size, low cost, and ease of manufacturing (Khedri, Ramezani, Rafatpanah, & Abnous, 2018).…”

Section: Fluorescence Aptamer Sensorsmentioning

confidence: 99%

Fluorescence‐based quantitative platform for ultrasensitive food allergen detection: From immunoassays to DNA sensors

Qian

Zhou

et al. 2020

Comp Rev Food Sci Food Safe

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Food allergies are global health issue with an increasing prevalence that affect food safety; hence, food allergen detection, labeling, and management are considered to be important priorities in the food industry. In this critical review, we provide a comprehensive overview of several fluorescence-based platforms based on different biorecognition ligands, such as antibodies, DNA, aptamers, and cells, for food allergen quantification. Traditional analytical methods are generally unsuitable for food manufacturers to accomplish the real-time identification of food allergens in food products. Therefore, it is important to develop simple, rapid, inexpensive, accurate, and sensitive methods to improve user accessibility. A fluorescence-based quantitative platform provides an excellent detection platform for food allergens because of its high sensitivity. This review summarizes the traditional antibody-based fluorescent techniques for food allergen detection, such as the time-resolved fluoroimmunoassay , immunofluorescence imaging, fluorescence enzyme-linked immune sorbent assay, flow injection fluoroimmunoassay, and fluorescence immunosensors. However, these methods suffer from disadvantages such as the significant rate of false-positive and false-negative results due to antibody cross-reactivity with nontarget food components in the complex food matrix and epitope degradation during food processing. Hence, different types of fluorescence-based immunoassays are suitable for standardization and quantification of allergens in fresh foods. In addition, we summarize new fluorescence-based quantitative platforms, including fluorescence genosensors, fluorescence cell sensors, and fluorescence aptamer sensors. With the advantages of high sensitivity and simple operation, fluorescence biosensors will have great potential in the future and could provide portable methods for multiallergen real-time detection in complex food systems.

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Advances in aptamer screening technologies

Cited by 93 publications

References 179 publications

Tumor microenvironment responsive drug delivery systems

Tumor microenvironment responsive drug delivery systems

Advances in detection of infectious agents by aptamer-based technologies

Fluorescence‐based quantitative platform for ultrasensitive food allergen detection: From immunoassays to DNA sensors

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