Advanced Sample Preparation Techniques for Pesticide Residues Determination by HRMS Analysis

Zanella, Renato; Prestes, Osmar D.; Bernardi, Gabrieli; Adaime, Martha B.

doi:10.1016/b978-0-12-809464-8.00005-1

Cited by 5 publications

(5 citation statements)

References 138 publications

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“…One strategy to minimize the matrix effect is the separation of analytes from matrix components during sample treatment, being the most frequently used for pesticide analysis the well‐known Quick Easy Effective Rugged and Safe method (QuEChERS). However, although an intensive clean‐up can reduce the matrix effect, this step may cause negative effects for some target compounds such as low recoveries, as well as being insufficient to remove matrix coextractives from the sample (Zanella et al, 2017).…”

Section: Application Of 2d‐lc–ms To Food Analysismentioning

confidence: 99%

Comprehensive and heart‐cutting multidimensional liquid chromatography–mass spectrometry and its applications in food analysis

Caño-Carrillo

Gilbert-López

Montero

et al. 2023

Mass Spectrometry Reviews

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In food analysis, conventional one‐dimensional liquid chromatography methods sometimes lack sufficient separation power due to the complexity and heterogeneity of the analyzed matrices. Therefore, the use of two‐dimensional liquid chromatography (2D‐LC) turns out to be a powerful tool to consider, especially when coupled to mass spectrometry (MS). This review presents the most remarkable 2D‐LC–MS food applications reported in the last 10 years, including a critical discussion of the multiple approaches, modulation strategies as well as the importance of the optimization of the different analytical aspects that will condition the 2D‐LC–MS performance. The presence of contaminants in food (food safety), the food quality, and authenticity or the relationship between the beneficial effects of food and human health are some of the fields in which most of the 2D‐LC–MS applications are mainly focused. Both heart‐cutting and comprehensive applications are described and discussed in this review, highlighting the potential of 2D‐LC–MS for the analysis of such complex samples.

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Section: Application Of 2d‐lc–ms To Food Analysismentioning

confidence: 99%

Comprehensive and heart‐cutting multidimensional liquid chromatography–mass spectrometry and its applications in food analysis

Caño-Carrillo

Gilbert-López

Montero

et al. 2023

Mass Spectrometry Reviews

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“…However, this technique has to cope with another challenge. The so-called matrix effects (ME) are a major drawback when liquid chromatography-mass spectrometry is used. − Compounds co-eluting with analytes can affect the analytes’ ionization process, leading to changes in the signal intensities. ,, This can either lead to suppression or enhancement of the signal intensities. ,,,− Compounds that originate from the sample and are still present in the extract after sample processing are referred to as endogenous compounds. Such compounds can be, e.g., lipids, peptides, or carbohydrates. , However, exogenous compounds that originate, e.g., from additives of the mobile phase and reagents of sample processing or are released during extraction (e.g., phthalates) can cause ME too. ,− ME can affect the qualitative analysis by leading to false positive or negative results.…”

Section: Introductionmentioning

confidence: 99%

“…The QuEChERS method is commonly used for sample preparation in pesticide residue analysis. − However, there are still matrix co-extractives that cannot be removed when applying the QuEChERS method. Therefore, different or additional clean-up steps are needed . An alternative approach seems to be two-dimensional liquid chromatography (2D-LC).…”

Section: Introductionmentioning

confidence: 99%

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A Comparison between a Two-Dimensional Liquid Chromatography System and a Traditional QuEChERS-LC Method with Regard to Matrix Removal and Matrix Effects in Pesticide Analysis Using Time-of-Flight Mass Spectrometry

Muehlwald

Meyburg

Rohn

et al. 2021

J. Agric. Food Chem.

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In this study, a fully automated two-dimensional liquid chromatography (2D-LC) system was used for the investigation of the clean-up effect and was compared with a traditional Quick Easy Cheap Effective Rugged and Safe (QuEChERS) liquid chromatography (LC) method. The focus of those investigations was on negative electrospray ionization (ESI) mode. For that purpose, matrix fingerprinting profiles were created. The results allowed a comparison of both methods regarding the estimation of the number and the polarity of detected compounds. Moreover, the results of the present study were compared with the results generated in positive ESI mode (presented in a previous study). Furthermore, the two methods were compared with regard to matrix effects (ME) of 321 analytes in positive ESI mode and 96 analytes in negative ESI mode. In general, fewer compounds could be detected when 2D-LC and/or the negative ESI mode was used. Especially, very polar compounds with m/z values >1000 could be separated and could not be detected anymore when 2D-LC was applied. Furthermore, the best results were obtained for most analytes when 2D-LC was used, although the extent of ME seemed to be higher with 2D-LC.

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“…In addition, the high-resolution mass spectrometry-based methods with several advantages, namely, excellent accurate mass and high sensitivity, have been introduced for screening of residual pesticides in food matrices [9]. Several sample preparation procedures have been reported, for example, liquid-liquid extraction and solid-phase extraction for such kind of analytes [16]. Besides, QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) sample preparation method has been known as the most powerful for removing isobaric and nonisobaric interference compounds in the food matrices.…”

Section: Introductionmentioning

confidence: 99%

Multiresidue Pesticides Analysis of Vegetables in Vietnam by Ultrahigh-Performance Liquid Chromatography in Combination with High-Resolution Mass Spectrometry (UPLC-Orbitrap MS)

Vu-Duc

Quang

Le-Minh

et al. 2019

Journal of Analytical Methods in Chemistry

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An ultrahigh-performance liquid chromatography in combination with high-resolution mass spectrometry Thermo Q-Extractive Focus Orbitrap MS has been introduced for analysis of multiclass pesticides in vegetable samples collected in Hanoi, Vietnam. Multiclass pesticides were separated on the Thermo Hypersil Gold PFP column utilizing a gradient of the mobile phase consisting of 5 mM ammonium formate, 0.1% formic acid in deionized water, and methanol. The target analytes were detected in the full-scan mode on Thermo Scientific Q-Exactive Focus Orbitrap MS for quantitation at the optimum operating conditions. These conditions included, but not limit to, the resolution of 70000 at the full width at half maximum in both positive and negative mode, mass range from 80 to 1000 m/z, and optimized parameters for the heated electrospray ionization source. The identification of the analytes in real samples was based on retention times, mass to charge ratios, mass accuracies, and MS/MS spectra at the confirmation mode with the inclusion list of target analytes. The mass accuracies of target analytes were from −4.14 ppm (dinotefuran) to 1.42 ppm (cinosulfuron) in the neat solvent and from −3.91 ppm (spinosad D) to 1.29 ppm (cinosulfuron) in the matrix-matched solution. Target analytes in the vegetable-based matrix were extracted by the QuEChERS method. Some critical parameters of the analytical method such as linearity, repeatability, limit of detection, and limit of quantitation have been evaluated and implemented. Excellent LOD and LOQ of the developed method were achieved at the range of 0.04–0.85 and 0.13–2.9 μg·kg−1, respectively. Intraday and interday repeatability of the analytical signal (peak area, n=6) of the developed method were below 3% and 10%, correspondingly. The matrix effect, extraction recovery, and overall recovery were fully investigated by spiking experiments. Experimental results demonstrated that the ionization suppression or enhancement was the main contribution on the overall recoveries of target analytes. Finally, the in-house validated method was applied to pesticides screening in vegetables samples in local villages in Hanoi, Vietnam. The concentrations of all target analytes were below limit of quantitation and lower than US-FDA or EU maximum residue levels.

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Advanced Sample Preparation Techniques for Pesticide Residues Determination by HRMS Analysis

Cited by 5 publications

References 138 publications

Comprehensive and heart‐cutting multidimensional liquid chromatography–mass spectrometry and its applications in food analysis

Comprehensive and heart‐cutting multidimensional liquid chromatography–mass spectrometry and its applications in food analysis

A Comparison between a Two-Dimensional Liquid Chromatography System and a Traditional QuEChERS-LC Method with Regard to Matrix Removal and Matrix Effects in Pesticide Analysis Using Time-of-Flight Mass Spectrometry

Multiresidue Pesticides Analysis of Vegetables in Vietnam by Ultrahigh-Performance Liquid Chromatography in Combination with High-Resolution Mass Spectrometry (UPLC-Orbitrap MS)

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