Advanced biosensors for detection of pathogens related to livestock and poultry

Abstract

Infectious animal diseases caused by pathogenic microorganisms such as bacteria and viruses threaten the health and well-being of wildlife, livestock, and human populations, limit productivity and increase significantly economic losses to each sector. The pathogen detection is an important step for the diagnostics, successful treatment of animal infection diseases and control management in farms and field conditions. Current techniques employed to diagnose pathogens in livestock and poultry include classical plate-based methods and conventional biochemical methods as enzyme-linked immunosorbent assays (ELISA). These methods are time-consuming and frequently incapable to distinguish between low and highly pathogenic strains. Molecular techniques such as polymerase chain reaction (PCR) and real time PCR (RT-PCR) have also been proposed to be used to diagnose and identify relevant infectious disease in animals. However these DNA-based methodologies need isolated genetic materials and sophisticated instruments, being not suitable for in field analysis. Consequently, there is strong interest for developing new swift point-of-care biosensing systems for early detection of animal diseases with high sensitivity and specificity. In this review, we provide an overview of the innovative biosensing systems that can be applied for livestock pathogen detection. Different sensing strategies based on DNA receptors, glycan, aptamers and antibodies are presented. Besides devices still at development level some are validated according to standards of the World Organization for Animal Health and are commercially available. Especially, paper-based platforms proposed as an affordable, rapid and easy to perform sensing systems for implementation in field condition are included in this review.

Deteksi Molekuler Mikroorganisme Patogen pada Bahan Pangan dengan Metode RT-PCR

“…Namun, prosedur standar tersebut membutuhkan waktu yang lama untuk dapat diketahui hasilnya (Zhao et al, 2016). Metode identifikasi konvensional meliputi serangkaian prosedur subkultur dan identifikasi biotipe-serotipe yang meskipun memberikan hasil yang sensitif (Vidic et al, 2017), tetapi membutuhkan waktu yang relatif lama (Naravaneni & Jamil, 2005).…”

The Detection Method ofEscherichia coliin Water Resources: A Review

“…Most detection technologies are revolving around the measurement of optical, electrochemical and piezoelectric [31]. Application and technologies in biosensors for E. coli detection water environmental analysis had reviewed in many articles [30][31][32][33][34][35]. The sensitivity of most biosensor seems has significantly increased in recent years, but it still has some shortcoming and needs more improvement to increase the sensitivity.…”

A Short Review of the Architecture and Computational Analysis in the Design of Graphene Based Bioelectronic Devices

“…• Enzyma�c fuel cell (3) • Microbial fuel cell (1) • Bioelectronic nose (7,8) • Bioelectronic tongue (7,8) • Bioelectronic eyes (7,8) • Fluidtrobe (80) • U tube concept (80) • Skin patches (4,5) • DNA extrac�on chip (4) • Glucose • monitoring (5,6) • Electrocardiography (ECG) (6) • Ac�ve exoskeleton (10) • Passive upper limb (10) • Passive lower limb (10) • Kidney mimic (9) • Liver mimic (9) • Heart mimic (9) • Lung mimic (9) (Anti-IgG) is anchored on gold nanoparticles (AuNPs). (40)(41)(42) The AuNPs are spread over a thermally reduced graphene oxide (TrGO) membrane as the channel material of the FET; these nanoparticles have shown specific recognition ability for IgG.…”

“…(4)(5)(6) For applications in artificial perception, several types of bioelectronic devices such as the bioelectronic nose, brain, and tongue have been demonstrated. (7,8) In the past decade, on-chip bioelectronic devices with the unique function of mimicking human organs such as the kidney, lung, heart, and liver have been developed for use in medical treatment. (9) Bioelectronic devices have also found their way into the development of exoskeletons for which a passive exoskeleton and active limbs have been designed and fabricated.…”