Adulticidal &amp; larvicidal efficacy of three neonicotinoids against insecticide susceptible &amp; resistant mosquito strains

Uragayala, Sreehari; Verma, Vaishali; Elamathi, N.; Velamuri, Poonam Sharma; Raghavendra, Kamaraju

doi:10.4103/0971-5916.176624

Cited by 25 publications

(21 citation statements)

References 17 publications

(16 reference statements)

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“…Shrimp eggs were added into the hatching chamber while the lamp above (light) attracted the hatched shrimps. The shrimps were left for 2 d to hatch and mature as nauplii (larva) and were used for the bioassay and the standard solution was made by serial dilutions [31][32][33] (Figure 2).…”

Section: Brine Shrimp Bioassaymentioning

confidence: 99%

Evaluation of Pistia stratiotes fractions as effective larvicide against Anopheles mosquitoes

Ugya

Isiyaku

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Mosquito are well-known vectors that cause diseases particularly malaria and filariasis which are detrimental to human health. These vectors occur mainly in tropical countries where more than 2 billion people live in endemic regions with about one million deaths been claimed yearly from malaria and filariasis. The study is aimed at evaluating the larvicidal activity of Pistia stratiotes fractions on Anopheles mosquitoes (Diptera: Culicidae). The ethyl acetate extract of P. stratiotes was obtained through percolation process and was chromatographed to yield nine fractions. The larvicidal activity of each of the nine fractions was tested in triplicates by exposing the larvae to 500, 250, 125, 62.5 and 31.3 mg/ml, respectively. Phytochemical screening of the nine fractions revealed the presence of alkaloids, flavonoids, glycosides and phlobatannins in varying quantities. The result obtained shows that fraction E has the highest lethal effect on the Anopheles larvae at LC 50 ¼14.81 mg/ml and was weakly effective at 602.03 mg/ml on brine shrimp larvae. The gas chromatography mass spectrometry analysis of fraction E revealed the presence of 35 pre-cursor compounds. Hence, ethyl acetate fractions of P. stratiotes could be an effective larvicide against Anopheles mosquito larvae as it has been found to be harmless to other aquatic organisms. Further work should be done on other aquatic weeds that have larvicidal potential to isolate the bioactive compounds. ARTICLE HISTORY

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Section: Brine Shrimp Bioassaymentioning

confidence: 99%

Evaluation of Pistia stratiotes fractions as effective larvicide against Anopheles mosquitoes

Ugya

Isiyaku

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…The evaluation of the efficacy of different neonicotinoids against three different strains of mosquitoes revealed efficacy of imidacloprid against Ae. aegypti with much higher lethal values; LC 50 and LC 90 as 0.558 ppm and 6.135 ppm [35].…”

Section: Discussionmentioning

confidence: 95%

Susceptibility Status of Aedes aegypti L. Against Different Classes of Insecticides in New Delhi, India to Formulate Mosquito Control Strategy in Fields

Samal¹,

Kumar²

2018

TOPARAJ

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Background: Mosquito control is a major concern throughout the world because of rising cases of mosquito-borne diseases. The outbreak of Zika, Dengue and Chikungunya has caused grave situations raising urgent need to control Aedes aegypti. Moreover, extensive use of synthetic insecticides in mosquito control programs has resulted in high levels of insecticide resistance leading to the use of magnified concentrations, impacting human health and environment adversely. The knowledge about current status of the insecticide susceptibility against Ae. aegypti could help to devise mosquito control strategy. Objective: Present study evaluates the larvicidal potential of thirteen insecticides belonging to seven different classes; organochlorines, organophosphates, carbamates, pyrethroids, neonicotinoids, avermectins and secondary metabolites; against early fourth instars of Ae. aegypti. Materials and Methods: The insecticide susceptibility was evaluated as per WHO protocol. Fatality counts were made after 24h of exposure; and the LC50, LC90 and other statistical parameters were computed by probit-regression analysis. Results: The data reveals the maximum efficacy of pyrethroids and fenitrothion, with lethal values less than 0.001 ppm. Avermectins, organochlorines and carbamates were moderately toxic, while neonicotinoid posed appreciable toxicity. In contrast, berberine, a secondary plant metabolite was found inefficient. The larvicidal efficacy of tested insecticides against Ae. aegypti was found in the decreasing order of pyrethroids > organophosphates > avermectins > organochlorines > carbamates > neonicotinoids > secondary metabolites. Conclusion: Present investigations explore various toxicants as Dengue vector control agents in order to devise a suitable control strategy for mosquito control in fields.

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“…To explore further, we analyzed SNVs in two gene families -the voltage-gated sodium channel (VGSC), a known target of pyrethroid insecticides [11,33] and fourteen nicotinic acetylcholine receptors (nAChr) α1-10 and β1-4 of the Cys-loop ligand-gated ion channel (Cys-loop LGIC) family, which are being developed as larvicidal targets [11,34]. While the 5 SNVs in the sequence of AEBAN2 VGSC did not alter amino acid sequence as compared with AaegL5 VGSC, the coding sequence of VGSC from AEBAN1 showed 16 SNVs resulting in three amino acid changes, namely S989P, V1016G and V1704G ( Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

Whole Genome Sequences ofAedes aegypti(Linn.) Field Isolates from Southern India

Bernard

Moudgalya

Reegan

et al. 2020

Preprint

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Aedes spp. mosquitoes are a major health concern as they transmit several viral pathogens resulting in millions of deaths annually around the world. This is compounded by the emergence of insecticide-resistant strains and global warming, which could expose more than half of the world's population to Aedes-borne diseases in the future. Therefore, a comprehensive understanding of vector biology and the genomic basis of phenotypes such as insecticide resistance in natural populations are of paramount importance. Here, we sequenced the genome of Aedes aegypti mosquitos sampled from dengue-endemic areas and investigated the genetic variations between the previously reported laboratory-reared strain and our field isolates. The mosquito genomic DNA was used for paired-end sequencing using the Illumina platform. The reads were used for template-based assembly and mapped to the Aedes aegypti reference genome. Stringent parameters and multiple variant calling methods were used to identify unique single nucleotide variants (SNVs) and insertions-deletions (indels) and mapped to the Aedes chromosomes to create a draft consensus genome. Gene Ontology analyses was performed on the variant-enriched genes while two gene families involved in insecticide resistance were used for comparative sequence and phylogenetic analyses. Comparative sequence variant analyses showed that the majority of the high-quality variants in our samples mapped to non-coding regions of the genome, while gene ontology analyses of genic variants revealed enrichment of terms relevant to drug binding and insecticide resistance. Importantly, one mutation implicated in pyrethroid resistance was found in one Aedes sample. This is the first report of genome sequences of A. aegypti field isolates from India which reveals variants specific to the wild population. This is a useful resource which will facilitate development of robust integrated vector control strategies for management of Aedes-borne diseases through genetic manipulation of local mosquito populations.

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Adulticidal & larvicidal efficacy of three neonicotinoids against insecticide susceptible & resistant mosquito strains

Cited by 25 publications

References 17 publications

Evaluation of Pistia stratiotes fractions as effective larvicide against Anopheles mosquitoes

Evaluation of Pistia stratiotes fractions as effective larvicide against Anopheles mosquitoes

Susceptibility Status of Aedes aegypti L. Against Different Classes of Insecticides in New Delhi, India to Formulate Mosquito Control Strategy in Fields

Whole Genome Sequences ofAedes aegypti(Linn.) Field Isolates from Southern India

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