Adsorption of immunogamma globulin onto various synthetic calcium hydroxyapatite particles

Kandori, Kazuhiko; Miyagawa, Kaori; Ishikawa, Tatsuo

doi:10.1016/j.jcis.2004.01.069

Cited by 68 publications

(38 citation statements)

References 32 publications

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“…This length and width was used for the monolayer coverage calculations, and thus it seems likely that laminin-1 was lying flat on the surface, similar to what was found on titania substrata (Santos et al 2011). The results for IgG differed from previous reports, where the adsorption was only 0.5 mg m -2 (Kandori et al 2004). However comparisons are difficult due to different experimental procedures, as well as different buffers and adsorption times.…”

Section: Total Adsorbed Amounts Adsorption Of Hsa Igg and Laminin-1 mentioning

confidence: 67%

“…be differences in chemical composition of the two surfaces. The Ca/P ratio differ between the two surfaces (1.6 for spin-coated and 1.2 for sintered HA), but previous studies have shown that adsorption of IgG and BSA are only weakly dependent on the Ca/P ratio (Kandori et al 2004). However, from the XPS data (not shown) it was revealed that the spin-coated surface contains titanium and aluminium, which is absent for the sintered one.…”

Section: Relation Between Ha Surface Characteristics and Competitive mentioning

confidence: 86%

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Adsorption of HSA, IgG and laminin-1 on model hydroxyapatite surfaces – effects of surface characteristics

et al. 2012

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Ellipsometry and mechanically assisted sodium dodecyl sulphate elution was utilized to study the adsorption of human serum albumin (HSA), human immunoglobulin G (IgG), laminin-1, as well as competitive adsorption from a mixture of these proteins on spin-coated and sintered hydroxyapatite (HA) surfaces, respectively. The HA surfaces were characterized concerning wettability and roughness by means of water contact angles and atomic force microscopy, respectively. Both surface types were hydrophilic, and the average roughness (Sa) and surface enlargement (Sdr) were lower for the sintered compared to the spin-coated HA surfaces. The adsorbed amounts on the sintered HA increased as follows: HSA < laminin-1 < IgG < protein mixture. For the competitive adsorption experiments, the adsorbed fractions increased accordingly: HSA < laminin-1 < IgG on both types of HA substrata. However, a higher relative amount of HSA and laminin-1 and a lower relative amount of IgG was found on the spin-coated surfaces compared to on the sintered ones.The effects observed could be ascribed to differences in surface roughness and chemical composition between the two types of HA substrata, and could have an influence on future implant surface coatings.

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Section: Total Adsorbed Amounts Adsorption Of Hsa Igg and Laminin-1 mentioning

confidence: 67%

Section: Relation Between Ha Surface Characteristics and Competitive mentioning

confidence: 86%

Adsorption of HSA, IgG and laminin-1 on model hydroxyapatite surfaces – effects of surface characteristics

et al. 2012

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“…Its biocompatibility is attributed to its protein adsorption [20][21][22][23]. An HAp sensor has been fabricated by our group using electrophoretic deposition, and the adsorption of single and multiple proteins has been studied using the quartz crystal microbalance with dissipation (QCM-D) technique [24-28, 43, 46].…”

Section: Introductionmentioning

confidence: 99%

Competitive adsorption of fibronectin and albumin on hydroxyapatite nanocrystals

Tagaya

Ikoma

Hanagata

et al. 2011

Science and Technology of Advanced Materials

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Competitive adsorption of two-component solutions containing fibronectin (Fn) and albumin (Ab) on hydroxyapatite (HAp) nanocrystals was analyzed in situ using the quartz crystal microbalance with dissipation (QCM-D) technique. Adsorption of the one-component protein (Fn or Ab) and the two-component proteins adjusted to different molar ratios of Fn to Ab at a fixed Fn concentration was investigated. The frequency shift ( f ; Hz) and the dissipation energy shift ( D) were measured with the QCM-D technique, and the viscoelastic changes of adlayers were evaluated by the saturated D/ f value and the Voigt-based viscoelastic model. For the adsorption of the one-component protein, the Fn adlayer showed a larger mass and higher viscoelasticity than the Ab adlayer, indicating the higher affinity of Fn on HAp. For the adsorption of the two-component proteins, the viscoelastic properties of the adlayers became elastic with increase in Ab concentration, whereas the adsorption mass was similar to that of Fn in the one-component solution regardless of the Ab concentration. The specific binding mass of the Ab antibody to the adlayers increased with increase in Ab concentration, whereas that of the Fn antibody decreased. Therefore, Fn preferentially adsorbs on HAp and Ab subsequently interacts with the adlayers, indicating that the interfacial viscoelasticity of the adlayers was dominated by the interaction between Fn and Ab.

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“…Hydroxyapatite (Ca 10 (PO 4 ) 6 (OH) 2 ; HAp) is a biocompatible ceramic used as a bone filling material together with collagen [20][21][22][23] and as a drug delivery carrier [24][25][26][27]. Its biocompatibility can be attributed to its mild adsorption of proteins; hence, this adsorption behavior has been widely investigated [28,29]. HAp nanocrystal sensors used in the QCM-D technique were recently fabricated by our group by electrophoretic deposition and ultrasonic irradiation [30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Reusable hydroxyapatite nanocrystal sensors for protein adsorption

Tagaya

Ikoma

Hanagata

et al. 2010

Science and Technology of Advanced Materials

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The repeatability of the adsorption and removal of fibrinogen and fetal bovine serum on hydroxyapatite (HAp) nanocrystal sensors was investigated by Fourier transform infrared (FTIR) spectroscopy and quartz crystal microbalance with dissipation (QCM-D) monitoring technique. The HAp nanocrystals were coated on a gold-coated quartz sensor by electrophoretic deposition. Proteins adsorbed on the HAp sensors were removed by (i) ammonia/hydrogen peroxide mixture (APM), (ii) ultraviolet light (UV), (iii) UV/APM, (iv) APM/UV and (v) sodium dodecyl sulfate (SDS) treatments. FTIR spectra of the reused surfaces revealed that the APM and SDS treatments left peptide fragments or the proteins adsorbed on the surfaces, whereas the other methods successfully removed the proteins. The QCM-D measurements indicated that in the removal treatments, fibrinogen was slowly adsorbed in the first cycle because of the change in surface wettability revealed by contact angle measurements. The SDS treatment was not effective in removing proteins. The APM or UV treatment decreased the frequency shifts for the reused HAp sensors. The UV/APM treatment did not induce the frequency shifts but decreased the dissipation shifts. Therefore, we conclude that the APM/UV treatment is the most useful method for reproducing protein adsorption behavior on HAp sensors.

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Adsorption of immunogamma globulin onto various synthetic calcium hydroxyapatite particles

Cited by 68 publications

References 32 publications

Adsorption of HSA, IgG and laminin-1 on model hydroxyapatite surfaces – effects of surface characteristics

Adsorption of HSA, IgG and laminin-1 on model hydroxyapatite surfaces – effects of surface characteristics

Competitive adsorption of fibronectin and albumin on hydroxyapatite nanocrystals

Reusable hydroxyapatite nanocrystal sensors for protein adsorption

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