Competitive adsorption of fibronectin and albumin on hydroxyapatite nanocrystals

Abstract

Competitive adsorption of two-component solutions containing fibronectin (Fn) and albumin (Ab) on hydroxyapatite (HAp) nanocrystals was analyzed in situ using the quartz crystal microbalance with dissipation (QCM-D) technique. Adsorption of the one-component protein (Fn or Ab) and the two-component proteins adjusted to different molar ratios of Fn to Ab at a fixed Fn concentration was investigated. The frequency shift ( f ; Hz) and the dissipation energy shift ( D) were measured with the QCM-D technique, and the viscoelastic changes of adlayers were evaluated by the saturated D/ f value and the Voigt-based viscoelastic model. For the adsorption of the one-component protein, the Fn adlayer showed a larger mass and higher viscoelasticity than the Ab adlayer, indicating the higher affinity of Fn on HAp. For the adsorption of the two-component proteins, the viscoelastic properties of the adlayers became elastic with increase in Ab concentration, whereas the adsorption mass was similar to that of Fn in the one-component solution regardless of the Ab concentration. The specific binding mass of the Ab antibody to the adlayers increased with increase in Ab concentration, whereas that of the Fn antibody decreased. Therefore, Fn preferentially adsorbs on HAp and Ab subsequently interacts with the adlayers, indicating that the interfacial viscoelasticity of the adlayers was dominated by the interaction between Fn and Ab.

In situ QCM-D study of nano-bio interfaces with enhanced biocompatibility

“…For the HAp surface, Ab and Fn adsorption in a complex fashion has been reported by two-component adsorption. 70 As judged from the ΔD-Δf plots in Figure 5, Ab interacts with Fn due to interfacial changes in elasticity using Voigt-based viscoelastic analysis and an antibody-binding technique.…”

“…With an increase in the albumin concentration, the ΔD sat /Δf sat value decreased from − 14.5 ± 1.9 × 10 -8 to − 6.3 ± 1.6 × 10 -8 , indicating that the viscoelastic behavior of the Anfibronectin compound adlayer changed to elastic with increasing albumin concentration. Reprinted with permission from Tagaya et al70 …”

Effects of human fibronectin and human serum albumin sequential adsorption on preosteoblastic cell adhesion

“…14 Fn is known to modulate a number of cell behaviors, including adhesion, migration, and differentiation. [20][21][22][23][24] Sousa et al have investigated the influence of Fn-Alb coadsorbed layers (on titania) on the adhesion and morphology of preosteoblastic MC3T3-E1 cells, and note that while cell adhesion generally correlates with extent of Fn adsorption, the Fn effect can be enhanced in the presence of a modest amount of Alb. Blood proteins such as albumin are generally much more concentrated in biological solution than are matrix proteins.…”

Effective Surface Functionalization of Carbon Fibers for Fiber/Polymer Composites with Tailor‐Made Interfaces

“…In the biomedical field, it is suggested that the high strength of CF can allow its use as implants, so that the coating with a biocompatible apatite (for example, hydroxyapatite (Hap): Ca 10 (PO 4 ) 6 (OH) 2 ) layer is needed. [61][62][63][64][65] Furthermore, magnetic nanoparticle (MNP) composites with a magnetite (Fe 3 O 4 ) core and HAp coating were prepared by using a precipitation method and a subsequent hydrothermal treatment as shown in Figure 4B. [60] We have also conducted studies on interfacial phenomena using HAp nanocrystals, including the investigation of the protein adsorption states on the surfaces.…”