Adsorption of HSA, IgG and laminin-1 on model hydroxyapatite surfaces – effects of surface characteristics

Svendsen, Ida; Santos, Olga; Sotres, Javier; Wennerberg, Ann; Breding, Karin; Arnebrant, Thomas; Lindh, Liselott

doi:10.1080/08927014.2011.653562

Cited by 12 publications

(8 citation statements)

References 50 publications

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“…Large m-HAP particles cannot be digested by osteoblastic SaOS-2 cells. The more hydrophobic and less protein absorbed surfaces of m-HAP particles in the m-HAP/Col composite ( 27 ) may detach the SaOS-2 cells with time, rendering strange morphological changes, as observed in our study ( Fig. 7 ).…”

Section: Discussionsupporting

confidence: 60%

Effects of apatite particle size in two apatite/collagen composites on the osteogenic differentiation profile of osteoblastic cells

Hatakeyama

Taira

Chosa

et al. 2013

International Journal of Molecular Medicine

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The development of new osteoconductive bone substitute materials is expected in medicine. In this study, we attempted to produce new hydroxylapatite (HAP)/collagen (Col) composites using two HAP particles of different sizes and porcine type I collagen. The two HAP particles were either nano-sized (40 nm in average diameter; n-HAP) or had macro-pore sizes of 0.5–1.0 mm in length with fully interconnected pores (m-HAP). The aim of this study was to investigate the effects of apatite particle size in two HAP/Col composites on the osteogenic differentiation profile in osteoblast-like cells (SaOS-2). We created a collagen control sponge (Col) and two HAP/Col composite sponges (n-HAP/Col and m-HAP/Col) using freeze-drying and dehydrothermal cross-linking techniques, and then punched out samples of 6 mm in diameter and 1 mm in height. The SaOS-2 cells were cultured on three test materials for 1, 2, 3 and 4 weeks. Total RNA was extracted from the cultured cells and the expression of osteogenic differentiation-related genes was evaluated by reverse transcription PCR (RT-PCR) using primer sets of alkaline phosphatase (ALP), type 1 collagen (COL1), bone sialoprotein (BSP) and osteocalcin precursor [bone gamma-carboxyglutamate (gla) protein (BGLAP)] genes, as well as the β-actin gene. The cells were also cultured on Col, n-HAP/Col and m-HAP/Col specimens for 1 and 4 weeks, and were then observed under a scanning electron microscope (SEM). The experimental results were as follows: RT-PCR indicated that osteogenic differentiation, particularly the gene expression of BSP, was most accelerated when the cells were cultured on n-HAP/Col specimens, followed by m-HAP/Col, whilst the weakest accelaeration was observed when the cells were cultured on Col specimens. As shown by the SEM images, the SaOS-2 cells were fibroblastic when cultured on Col specimens for up to 4 weeks; they were fibroblastic when cultured on n-HAP/Col specimens for 1 week, but appeared as spheroids, while actively phagocytizing n-HAP particles at 4 weeks; however, they appeared as deformed fibroblasts when cultured on m-HAP/Col specimens, detached from the particles. Despite limited experimental results, our study suggests that n-HAP/Col may be employed as a new osteoconductive bone substitute material.

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Section: Discussionsupporting

confidence: 60%

Effects of apatite particle size in two apatite/collagen composites on the osteogenic differentiation profile of osteoblastic cells

Hatakeyama

Taira

Chosa

et al. 2013

International Journal of Molecular Medicine

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“…The predominance of one of them over the others is closely related to the type of protein and to the chemical–physical features of the inorganic surface. Based on our results and on previous works about HA functionalization with proteins, in this system, the hydrophobic interactions can be discarded and it can be assumed that mainly electrostatic interactions between LF and the HA surface take place.…”

Section: Discussionmentioning

confidence: 92%

Effect of hydroxyapatite nanocrystals functionalized with lactoferrin in osteogenic differentiation of mesenchymal stem cells

Montesi

Panseri

Iafisco

et al. 2014

J. Biomed. Mater. Res.

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Lactoferrin (LF) is a bioactive glycoprotein that became recently interesting in the field of bone regeneration for its modulatory effect on bone cells. On the basis of this evidence this work aims to functionalize biomimetic hydroxyapatite (HA) nanocrystals with LF to study their effect on osteogenic differentiation of mesenchymal stem cells (MSCs). The orientation of LF on the HA surface was analyzed by spectroscopic and thermal techniques. Three samples with different amounts of LF attached to HA nanocrystals were tested in vitro. The combined effect of HA and LF on MSC proliferation and morphology, alkaline phosphatase (ALP) activity, and gene expression were evaluated at different time points. The sample with the lowest LF amount showed the best bioactivity probably due to the formation of a single layer of protein with a better molecular orientation. Coupling of HA-LF did not affect cell proliferation and morphology, while analysis of HA-LF on ALP activity and messenger RNA expression of the selected genes, demonstrated the role of HA-LF in the induction of osteogenic markers. HA-LF represents a promising system to be used to manufacture bioactive functional materials in tissue engineering (as scaffolds, injectable cements, or coatings for metallic implants) with enhanced anabolic activity to treat bone diseases.

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“…Fan et al [ 61 ] used quartz crystal microbalance to determine the adsorption of bovine serum albumin (BSA) and Fib on the L- and D-selenocystine (L-Se/D-Se) immobilized TiO 2 films, and found that surface chirality led to different steric interactions between the negatively charged L-Se/D-Se surfaces and the negatively charged BSA and Fib, which in turn influenced hydrogen bonding and hydrophobic interaction, and eventually influenced protein adsorption. Svendsen et al [ 62 ] used ellipsometry to study the competitive adsorption of human serum albumin (HSA), human immunoglobulin G (IgG) and laminin-1 on the spin-coated and sintered hydroxyapatite (HA) surfaces. The results showed that the differences in surface roughness and chemical composition (the spin-coated HA surface contained titanium and aluminum, whereas the sintered surface contained sodium) between two types of HA substratum contributed to different adsorption properties of proteins.…”

Section: Resultsmentioning

confidence: 99%

A proteomics study to explore the role of adsorbed serum proteins for PC12 cell adhesion and growth on chitosan and collagen/chitosan surfaces

Lü

Zhang

Huang

et al. 2018

Regenerative Biomaterials

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The aim of this article is to apply proteomics in the comparison of the molecular mechanisms of PC12 cell adhesion and growth mediated by the adsorbed serum proteins on the surfaces of chitosan and collagen/chitosan films. First, the chitosan and the collagen/chitosan films were prepared by spin coating; and their surface morphologies were characterized by scanning electron microscopy, X-ray energy dispersive spectroscopy, contact angle measurement and Fourier transform infrared spectroscopy. Subsequently, cell proliferation experiments on two materials were performed and the dynamic curves of protein adsorption on their surfaces were measured. Then, proteomics and bioinformatics were used to analyze and compare the adsorbed serum proteins on the surfaces of two biomaterials; and their effects on cell adhesion were discussed. The results showed that the optimum concentration of chitosan film was 2% w/v. When compared with chitosan film, collagen/chitosan film promoted the growth and proliferation of PC12 cells more significantly. Although the dynamic curves showed no significant difference in the total amount of the adsorbed proteins on both surfaces, proteomics and bioinformatics analyses revealed a difference in protein types: the chitosan surface adsorbed more vitronectin whereas collagen/chitosan surface adsorbed more fibronectin 1 and contained more cell surface receptor binding sites and more Leu-Asp-Val sequences in its surface structure; the collagen/chitosan surface were more conducive to promoting cell adhesion and growth.

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Adsorption of HSA, IgG and laminin-1 on model hydroxyapatite surfaces – effects of surface characteristics

Cited by 12 publications

References 50 publications

Effects of apatite particle size in two apatite/collagen composites on the osteogenic differentiation profile of osteoblastic cells

Effects of apatite particle size in two apatite/collagen composites on the osteogenic differentiation profile of osteoblastic cells

Effect of hydroxyapatite nanocrystals functionalized with lactoferrin in osteogenic differentiation of mesenchymal stem cells

A proteomics study to explore the role of adsorbed serum proteins for PC12 cell adhesion and growth on chitosan and collagen/chitosan surfaces

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