Adsorption of bovine serum albumin onto synthetic Fe-doped geomimetic chrysotile

Abstract

Synthetic stoichiometric and Fe-doped geomimetic chrysotile nanocrystals represent a reference standard to investigate the health hazard associated with mineral asbestos fibres. Experimental evidence suggests that the generation of reactive oxygen species and other radicals, catalysed by iron ions at the fibre surface, plays an important role in asbestos-induced cytotoxicity and genotoxicity. In this study, structural modification of bovine serum albumin (BSA) adsorbed onto synthetic chrysotile doped with different amounts of Fe has been investigated by Fourier transform infrared spectroscopy (FT-IR), thermal gravimetric analysis (TGA) and analytical pyrolysis coupled with gas chromatography-mass spectrometry. FT-IR data evidenced a marked increase in disordered structures like random coil and b-turn of BSA-nanocrystal adduct with 0.52 wt% of Fe doped. The TGA profile of the BSA revealed that its interaction with the synthetic chrysotile surface was strongly affected by the substitution of Fe into the chrysotile structure. The 2,5-diketopiperazine yields, formed upon thermal degradation of the polypeptide chain (pyrolysis-gas chromatography), changed when the BSA was adsorbed on the nanofibres. In general, results suggested that minute amount (less than 1 wt%) of Fe doping in chrysotile affected the protein-nanofibre interactions, supporting the role that this element may play in asbestos toxicity. The catalytic role of iron and the consequent unfolding of protein due to the structural surface modification of nanofibres were also evaluated.

Thermoanalytical Characterization and Catalytic Conversion of Deoiled Micro Algae and Jatropha Seed Cake

“…Adamiano et al established that small amounts of iron on chrysotile nanofiber decreased the DKP formation from bovine serum albumin during off-line pyrolysis. 44 A similar reduction was observed in the relative intensities of DKPs during the pyrolysis of DAC using Fe/FSM-16 catalyst. However, this change was not observed under other experimental conditions, as it can be seen in Table 3 and Figure S2.…”

“…The protein content of DAC and JSDC are 20.5% and 28.8%, respectively (Table ). Adamiano et al established that small amounts of iron on chrysotile nanofiber decreased the DKP formation from bovine serum albumin during off-line pyrolysis . A similar reduction was observed in the relative intensities of DKPs during the pyrolysis of DAC using Fe/FSM-16 catalyst.…”

Protein-Mediated Aqueous Synthesis of Stable Methylammonium Lead Bromide Perovskite Nanocrystals: Implications for Biological and Environmental Applications

“…In addition, thermal decomposition of the polypeptide chain in BSA generates a small shoulder at 225 °C (Figure S8c). 48,52 Interestingly, the HPNCs capped with BSA exhibit a thermal decomposition trend corresponding to the average of both pure BSA and Pure-HPs, further confirming the coexistence of the proteins and HPNCs in the sample.…”