modified ground quill, 2522% for composites with modified long fiber and 3206% for the composites with modified short fiber. The lysozyme test shows an improved in the degradability rate, the weight loss of the films at 21 days is reduced from 73% for chitosan-starch matrix up to 16% for the composites with 5 wt% of quill; but all films show a biodegradable character depending on keratin type and chemical modification. The outstanding properties related to the addition of treated keratin materials show that these natural composites are a remarkable alternative to potentiating chitosan-starch films with sustainable features.
Magnetic nanoparticles (MNPs) have great potential in biomedical applications because of their magnetic response offers the possibility to direct them to specific areas and target biological entities. Magnetic separation of biomolecules is one of the most important applications of MNPs because their versatility in detecting cancer biomarkers. However, the effectiveness of this method depends on many factors, including the type of functionalization onto MNPs. Therefore, in this study, magnetite nanoparticles have been developed in order to separate the 5′-nucleotidase enzyme (5eNT). The 5eNT is used as a bio-indicator for diagnosing diseases such as hepatic ischaemia, liver tumor, and hepatotoxic drugs damage. Magnetic nanoparticles were covered in a core/shell type with silica, aminosilane, and a double shell of silica-aminosilane. A ScFv (fragment antibody) and anti-CD73 antibody were attached to the coated nanoparticles in order to separate the enzyme. The magnetic separation of this enzyme with fragment antibody was found to be 28% higher than anti-CD73 antibody and the enzyme adsorption was improved with the double shell due to the increased length of the polymeric chain. Magnetite nanoparticles with a double shell (silica-aminosilane) were also found to be more sensitive than magnetite with a single shell in the detection of biomarkers.
Starch (potato), chitosan, and feather keratin are used for processing biodegradable films produced by extrusion. The morphology of the films is examined with a scanning electron microscope and showed the excellent dispersion of keratin. The dispersion is the result of compatibility between the polysaccharides and proteins, as well as the proper operation of the extrusion process. Water solubility of the starch-chitosan films decreased with an increase of keratin materials. The storage modulus increased up to 137% for the composites with unmodified ground quill, and by 192% for composites with modified ground quill. In a tensile test, the composites with unmodified and modified quill reached outstanding increments up to 8160 and 7250% in elastic modulus, respectively, compared to the matrix. They also reached up to 3800% and 3150% in maximum strength, respectively, compared to the matrix. The lysozyme test showed relevant changes in the degradability rate, because the weight loss of the films at 3 weeks decreased from 53% for starch-chitosan matrix and up to 34% for composites with 5 wt% of modified quill. The results corroborated that chicken feather materials can be useful for the development of a manufacturing process for starch composites, and the decomposition of starch-chitosan composites can be controlled depending on the content and type of keratin.
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