Administration of BAY 41-2272 prevents bladder dysfunction in nitric-oxide deficient rats

D’Ancona, Carlos Arturo Levi; Mónica, Fabíola Z.; Reges, Ricardo; Cohen, David E.; Silva, Fábio Henrique da; Nucci, Gilberto De; Antunes, Edson

doi:10.1590/s1679-45082010ao1789

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“…The same result was found in chronically L-NAME treated rats (35) and was due to DSM super sensitivity to muscarinic agonists via increases in the levels of [ 3 H] inositol phosphate (IP 3 ), accompanied by reduction of β 3 -adrenoceptor-mediated DSM relaxations (23). Aminoguanidine treatment decreased NVC in BOO mice, as shown in BOO rats treated with the same drug, due to attenuation in fibrosis.…”

Section: Discussionsupporting

confidence: 68%

Effects of nitric oxide inhibitors in mice with bladder outlet obstruction

et al. 2017

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Purpose To investigate the lower urinary tract changes in mice treated with L-NAME, a non-selective competitive inhibitor of nitric oxide synthase (NOS), or aminoguanidine, a competitive inhibitor of inducible nitric oxide synthase (iNOS), after 5 weeks of partial bladder outlet obstruction (BOO), in order to evaluate the role of constitutive and non-constitutive NOS in the pathogenesis of this experimental condition.Materials and Methods C57BL6 male mice were partially obstructed and randomly allocated into 6 groups: Sham, Sham + L-NAME, Sham + aminoguanidine, BOO, BOO + L-NAME and BOO + aminoguanidine. After 5 weeks, bladder weight was obtained and cystometry and tissue bath contractile studies were performed.Results BOO animals showed increase of non-voiding contractions (NVC) and bladder capacity, and also less contractile response to Carbachol and Electric Field Stimulation. Inhibition of NOS isoforms improved bladder capacity and compliance in BOO animals. L-NAME caused more NVC, prevented bladder weight gain and leaded to augmented contractile responses at muscarinic and electric stimulation. Aminoguanidine diminished NVC, but did not avoid bladder weight gain in BOO animals and did not improve contractile responses.Conclusion It can be hypothesized that chronic inhibition of three NOS isoforms in BOO animals leaded to worsening of bladder function, while selective inhibition of iNOS did not improve responses, what suggests that, in BOO animals, alterations are related to constitutive NOS.

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Section: Discussionsupporting

confidence: 68%