Adjuvant activity of Chinese herbal polysaccharides in inactivated veterinary rabies vaccines

Liu, Ye; Zhang, Shoufeng; Zhang, Fei; Hu, Rongliang

doi:10.1016/j.ijbiomac.2012.01.035

Cited by 21 publications

(16 citation statements)

References 32 publications

(29 reference statements)

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“…GUP possesses diverse pharmacological properties, including immune-modulatory, antioxidant, anti-viral, and anti-inflammatory [4–7]. Recently, GUP, as the primary active ingredient in G. uralensis Fisch , has drawn much attention, and has been increasingly utilized in animal feed additives, veterinary drugs, and vaccines [8]. …”

Section: Introductionmentioning

confidence: 99%

Optimized Extraction, Preliminary Characterization, and In Vitro Antioxidant Activity of Polysaccharides from Glycyrrhiza Uralensis Fisch

Chen

2017

Med Sci Monit

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BackgroundThis study performed optimized extraction, preliminary characterization, and in vitro antioxidant activities of polysaccharides from Glycyrrhiza uralensis Fisch.Material/MethodsThree parameters (extraction temperature, ratio of water to raw material, and extraction time) were optimized for yields of G. uralensis polysaccharides (GUP) using response surface methodology with Box-Behnken design (BBD). The GUP was purified using DEAE cellulose 32-column chromatography. The main fraction obtained from G. uralensis Fisch was GUP-II, which was composed of rhamnose, arabinose, galactose, and glucose monosaccharide, was screened for antioxidant properties using DP Hand hydroxyl radical scavenging assays. In addition, immunological activity of GUP-II was determined by nitric oxide and lymphocyte proliferation assays.ResultsOptimization revealed maximum GUP yields with an extraction temperature of 99°C, water: raw material ratio of 15: 1, and extraction duration of 2 h. GUP-II purified from G. uralensis Fisch had good in vitro DPPH and hydroxyl radical scavenging abilities. Immunologically, GUP-II significantly stimulated NO production in RAW 264.7 macrophages, and significantly enhanced LPS-induced lymphocyte proliferation.ConclusionsExtraction of GUP from G. uralensis Fisch can be optimized with respect to temperature, extraction period, and ratio of water to material, using response surface methodology. The purified product (GUP-II) possesses excellent antioxidant and immunological activities.

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Section: Introductionmentioning

confidence: 99%

Optimized Extraction, Preliminary Characterization, and In Vitro Antioxidant Activity of Polysaccharides from Glycyrrhiza Uralensis Fisch

Chen

2017

Med Sci Monit

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“…Others have shown that alkylamide extracts given in tablet form do reach the blood stream, supporting in vivo relevance [42]. Another recent study has shown that Echinacea polysaccharide was a strong adjuvant for the stimulation of accelerated and enhanced effects on rabies-neutralizing antibody responses in mice and dogs [43]. …”

Section: Discussionmentioning

confidence: 93%

Echinacea purpurea (L.) Moench modulates human T-cell cytokine response

Fonseca

Papanicolaou

Lin

et al. 2014

International Immunopharmacology

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The study objective was to evaluate the composition of a neutral and weakly acidic water-soluble extract from Echinacea purpurea (L.) Moench (EchNWA) previously shown to modify murine influenza infection, and to assess immunomodulatory effects on human T-cells. EchNWA extract from fresh aerial parts was extracted with water, ethanolic precipitation, and size-exclusion chromatography. The chemical profile of EchNWA was characterized by chromatography (size-exclusion, HPLC, GC–MS), and small molecule finger-print analysis performed by HPLC–PDA. Jurkat T-cells at high and low cell density were pretreated or not with doses of EchNWA, followed by activation with phorbol 12-myristate 13-acetate plus ionomycin (PMA+I). Interleukin-2 (IL-2) and interferon gamma (IFNg) cytokine secretions were measured by multi-cytokine luminex technology. Results showed that EchNWA contains 80% polysaccharides, predominantly a 10 kDa entity; phenolic compounds, cynarin, cichoric and caftaric acids, but no detectable alkylamides. Cytokine production required stimulation and was lower after PMA+I activation in high-density compared to low-density conditions. EchNWA mediated a strong dose-dependent enhancement of high-density T-cell production of IL-2 and IFNg response to PMA+I. EchNWA alone did not stimulate T-cells. EchNWA enhanced mean fluorescence intensity of IL-2 in Jurkat T-cells activated by PMA+1 or ionomycin alone. Conversely EchNWA mediated modest but significant suppression of IFNg response and reduced the percentage of CD25+ T-cells under low-density conditions. Conclusions are that EchNWA polysaccharides, but not phenolic compounds have dose-related adjuvant effects on human T-cell cytokine responses characterized by enhancing and suppressive effects that are regulated by T-cell density.

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“…Stevenson et al (2005) demonstrated that Echinacea is an effective modulator of the macrophage immune response. Moreover, EP is used as a helper substance (adjuvant) in veterinary rabies vaccines to activate antigen presenting cells and to stimulate these cells to produce more cytokines which activate lymphocytes producing specific antibodies (Liu et al 2012). Previous studies have evaluated the efficiency of different vaccine preparations against F. columnare infection and the humoral immune response induced by them (Grabowski et al 2004, Shoemaker et al 2007, Leal et al 2010.…”

Section: Discussionmentioning

confidence: 99%

Effect of dietary supplementation with Echinacea purpurea on vaccine efficacy against infection with Flavobacterium columnare in zebrafish (Danio rerio)

Guz¹,

Puk²,

Walczak³

et al. 2014

Polish Journal of Veterinary Sciences

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The effect of dietary Echinacea purpurea (EP) on the response of zebrafish (Danio rerio) to a Flavobacterium columnare vaccine was investigated. Two hundred D. rerio with an average weight of 290 ± 40 g were selected and fed different levels of E. purpurea (5 g kg -1 diet -group 1, 10 g kg -1 diet -group 2, 20 g kg -1 diet -group 3, 30 g kg -1 diet -group 4, and 0 g kg -1 diet -group 5). Experimental feeding was begun 3 weeks prior to bath immunization and continued until the end of the experiment. Twenty-eight days after immunization the fish were challenged by bath immersion with F. columnare at a concentration of 1x106 CFU/ml. The relative percent survival of the experimental groups (1, 2, 3, 4 and 5) was 5.0, 6.0, 30.0, 36.0 and 5.0, respectively. In conclusion, diet supplementation with E. purpurea may effectively enhance the response of zebrafish to a F. columnare vaccine.

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Adjuvant activity of Chinese herbal polysaccharides in inactivated veterinary rabies vaccines

Cited by 21 publications

References 32 publications

Optimized Extraction, Preliminary Characterization, and In Vitro Antioxidant Activity of Polysaccharides from Glycyrrhiza Uralensis Fisch

Optimized Extraction, Preliminary Characterization, and In Vitro Antioxidant Activity of Polysaccharides from Glycyrrhiza Uralensis Fisch

Echinacea purpurea (L.) Moench modulates human T-cell cytokine response

Effect of dietary supplementation with Echinacea purpurea on vaccine efficacy against infection with Flavobacterium columnare in zebrafish (Danio rerio)

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