Effect of dietary supplementation with Echinacea purpurea on vaccine efficacy against infection with Flavobacterium columnare in zebrafish (Danio rerio)

Abstract

The effect of dietary Echinacea purpurea (EP) on the response of zebrafish (Danio rerio) to a Flavobacterium columnare vaccine was investigated. Two hundred D. rerio with an average weight of 290 ± 40 g were selected and fed different levels of E. purpurea (5 g kg -1 diet -group 1, 10 g kg -1 diet -group 2, 20 g kg -1 diet -group 3, 30 g kg -1 diet -group 4, and 0 g kg -1 diet -group 5). Experimental feeding was begun 3 weeks prior to bath immunization and continued until the end of the experiment. Twenty-eight days after immunization the fish were challenged by bath immersion with F. columnare at a concentration of 1x106 CFU/ml. The relative percent survival of the experimental groups (1, 2, 3, 4 and 5) was 5.0, 6.0, 30.0, 36.0 and 5.0, respectively. In conclusion, diet supplementation with E. purpurea may effectively enhance the response of zebrafish to a F. columnare vaccine.

Adjuvant effects of medicinal herbs and probiotics for fish vaccines

“…Feeding purple coneflower ( Echinacea purpurea ) three weeks before bath immunization with Flavobacterium columnare demonstrated a protective effect in zebrafish following immersion challenge 28 days post immunization (Guz et al . ). The immunological status continued until four weeks post‐vaccination.…”

Echinacea plants as antioxidant and antibacterial agents: From traditional medicine to biotechnological applications

“…Three of the most used medicinal plant species belonging to the Echinacea genus are E. purpurea , E. angustifolia , and E. pallida , known all of them as purple coneflower, black sampson, pale purple coneflower, among others (Barnes, Anderson, Gibbons, & Phillipson, ; Barrett, ; Flannery, ; Gurib‐Fakim, ). The indigenous traditional healers of the native North American tribes such as Cheyenne, Choctaw, Dakota, Delaware, Fox Kiowa, Montana, Omaha Pawnee, Ponca, Sioux, and Winnebago prescribed different preparations of E. angustifolia , E. purpurea , and E. pallida for pain relief, skin inflammatory conditions, wound treatment, as an antidote against various poisons, snakebites, for symptoms associated with the common cold, infectious diseases, and among others (Barrett, ; Borchers, Keen, Stern, & Gershwin, ; Flannery, ; Gurib‐Fakim, ; Guz, Puk, Walczak, Oniszczuk, & Oniszczuk, ; Guz, Sopinska, & Oniszczuk, ). Echinacea spp.…”

“…Three of the most used medicinal plant species belonging to the Echinacea genus are E. purpurea, E. angustifolia, and E. pallida, known all of them as purple coneflower, black sampson, pale purple coneflower, among others (Barnes, Anderson, Gibbons, & Phillipson, 2005;Barrett, 2003;Flannery, 1999;Gurib-Fakim, 2006 (Barrett, 2003;Borchers, Keen, Stern, & Gershwin, 2000;Flannery, 1999;Gurib-Fakim, 2006;Guz, Puk, Walczak, Oniszczuk, & Oniszczuk, 2014;Guz, Sopinska, & Oniszczuk, 2011). Echinacea spp.…”

Infectious diseases in aquarium ornamental pet fish

“…Most of the bacteria that affect ornamental fish are classified as gram-negative. Species of genus Aeromonas, Citrobacter (Roberts et al, 2009), Edwardsiella (Lowry & Smith, 2007;Hawke et al, 2013;Humphrey et al, 1986), Francisella (Camus et al, 2013Colquhoun & Duodu, 2011;Lewisch et al, 2014), Flavobacterium (Declercq et al, 2013(Declercq et al, , 2015Guz et al, 2014;Verma et al, 2015), Plesiomonas (Nisha al., 2014), Shewanella (Pękala et al, 2015), and Vibrio are the most common genera (Abd El-Galil & Mohamed, 2012;Hashem & El-Barbary, 2013;Martins et al, 2010;Tendencia, 2004). The gram-negative species that are most commonly reported in ornamental aquarium fish are Aeromonas hydrophilla, Aeromonas veroni, Aeromonas caviae (Citarasu et al, 2011;Grim et al, 2013;Yucel et al, 2005), Citrobacter freundii (Gallani et al, 2016), Shevanella putrefaciens (Altun et al, 2014;Beaz-Hidalgo et al, 2015;Chen et al, 1997), and Flavobacterium columnare (Declercq et al, 2013).…”