Adipose‐derived stem cells: An appropriate selection for osteogenic differentiation

Shafaei, Hajar; Kalarestaghi, Hossein

doi:10.1002/jcp.29681

Cited by 44 publications

(38 citation statements)

References 123 publications

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“…Adipose-derived stem cells (ASCs) were used as a model cell source to investigate the cellular interactions on PCL/ EWP nanofibrous mats. ASCs were selected as they are frequently used in stem cell-based tissue engineering applications, as an abundant, readily available cell source [35][36][37][38]. ASCs were isolated from human subcutaneous adipose lipoaspirates under an Institutional Review Board approved (AU Clinical Research Ethical Committee #13-25-15) protocol with informed patient consent.…”

Section: Cell Isolation and Culturementioning

confidence: 99%

Facile modification of polycaprolactone nanofibers with egg white protein

Renkler

Ergene

Gokyer

et al. 2021

J Mater Sci: Mater Med

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Synthetic polymers remain to be a major choice for scaffold fabrication due to their structural stability and mechanical strength. However, the lack of functional moieties limits their application for cell-based therapies which necessitate modification and functionalization. Blending synthetic polymers with natural components is a simple and effective way to achieve the desired biological properties for a scaffold. Herein, nanofibrous mats made of polycaprolactone (PCL) and egg white protein (EWP) blend were developed and further evaluated for use as a scaffold for tissue engineering applications. Homogeneous distribution of EWP was achieved throughout the nanofibrous mats, as shown by immunohistochemistry. ATR-FTIR analysis and contact angle measurements have further confirmed the presence of EWP on the surface of the samples. The swelling test showed that PCL/EWP nanofibers have higher water uptake than PCL nanofibrous mats. Also, EWP addition on the nanofibrous mats resulted in an increase in the tensile strength and Young’s modulus of the mats, indicating that the presence of protein can greatly enhance the mechanical properties of the mats. A significantly higher, more uniform, and dispersed cell spreading was observed on days 7 and 14 than that on neat PCL mats, demonstrating the importance of providing the required cues for cell homing by the availability of EWP. Hence, EWP is shown to be a simple and low-cost source for the functionalization of PCL nanofibrous mats. EWP is, therefore, a facile candidate to enhance cellular interactions of synthetic polymers for a wide range of tissue engineering applications.

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Section: Cell Isolation and Culturementioning

confidence: 99%

Facile modification of polycaprolactone nanofibers with egg white protein

Renkler

Ergene

Gokyer

et al. 2021

J Mater Sci: Mater Med

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“…The nutrient source shows only minor impact on osteogenic differentiation of ASCs [ 110 ], which indicates that autologous serum could be a viable alternative. Comparing studies on ASCs can be difficult since protocols are often not standardized and this can influence the properties of the ASCs [ 111 , 112 ].…”

Section: Cell Therapymentioning

confidence: 99%

“…For osteogenesis, these are Runx2 and Osterix (Sp7 transcription factor) [ 113 , 114 ]. Regulation involves a complex network and several signaling pathways control osteogenic differentiation, among others bone morphogenetic protein (BMP)- [ 115 ], Notch- [ 116 ], Wnt- [ 112 ] and Hedgehog-signaling [ 117 ]. The Wnt signaling pathway is key as it serves as governor between ASC lineages.…”

Section: Osteogenic Stem Cell Differentiation Through Bioactive Factorsmentioning

confidence: 99%

The Role of Adipose Stem Cells in Bone Regeneration and Bone Tissue Engineering

Mende

Götzl

Kubo

et al. 2021

Cells

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Bone regeneration is a complex process that is influenced by tissue interactions, inflammatory responses, and progenitor cells. Diseases, lifestyle, or multiple trauma can disturb fracture healing, which might result in prolonged healing duration or even failure. The current gold standard therapy in these cases are bone grafts. However, they are associated with several disadvantages, e.g., donor site morbidity and availability of appropriate material. Bone tissue engineering has been proposed as a promising alternative. The success of bone-tissue engineering depends on the administered cells, osteogenic differentiation, and secretome. Different stem cell types offer advantages and drawbacks in this field, while adipose-derived stem or stromal cells (ASCs) are in particular promising. They show high osteogenic potential, osteoinductive ability, and immunomodulation properties. Furthermore, they can be harvested through a noninvasive process in high numbers. ASCs can be induced into osteogenic lineage through bioactive molecules, i.e., growth factors and cytokines. Moreover, their secretome, in particular extracellular vesicles, has been linked to fracture healing. The aim of this review is a comprehensive overview of ASCs for bone regeneration and bone tissue engineering.

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“…ASCs can also differentiate into osteoblasts and chondrocytes [14][15][16][17]. Because of their ability to multi-directionally differentiate, their extensive proliferative capacity, and low immunogenicity, ASCs have become an attractive source of seed cells for regenerative medicine and tissue engineering [18][19][20][21][22]. Therefore, researchers hope to transplant autologous ASCs from osteoporotic patients for the treatment of bone defects.…”

Section: Introductionmentioning

confidence: 99%

Role of Fzd6 in Regulating the Osteogenic Differentiation of Adipose-derived Stem Cells in Osteoporosis

Yao

Tao

et al. 2021

Preprint

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Objective: Although it has been demonstrated that adipose-derived stem cells (ASCs) from osteoporosis mice (OP-ASCs) exhibit impaired osteogenic differentiation potential, the molecular mechanism has not yet been elucidated. We found that Fzd6 was decreased in OP-ASCs compared with ASCs. This study investigates the effects and underlying mechanisms of Fzd6 in the osteogenic potential of OP-ASCs. Methods: Fzd6 expression in ASCs and OP-ASCs was measured by PCR gene chip. Fzd6 overexpression and silencing lentiviruses were used to evaluate the role of Fzd6 in the osteogenic differentiation of OP-ASCs. Real-time PCR (qPCR) and western blotting (WB) was performed to detect the expression of Fzd6 and bone-related molecules, including runt-related transcription factor 2 (Runx2) and osteopontin (Opn). Alizarin red staining and Alkaline phosphatase (ALP) staining was performed following osteogenic induction. Microscopic CT (Micro-CT), hematoxylin and eosin staining (H&E) staining, and Masson staining were used to assess the role of Fzd6 in osteogenic differentiation of osteoporosis (OP) mice in vivo.Results: Expression of Fzd6 was decreased significantly in OP-ASCs. Fzd6 silencing down-regulated the osteogenic ability of OP-ASCs in vitro. Overexpression of Fzd6 rescued the impaired osteogenic capacity in OP-ASCs in vitro. We obtained similar results in vivo.Conclusions: Fzd6 plays an important role in regulating the osteogenic ability of OP-ASCs both in vivo and in vitro. Overexpression of Fzd6 associated with the Wnt signaling pathway promotes the osteogenic ability of OP-ASCs, which provides new insights for the prevention and treatment of OP.

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Adipose‐derived stem cells: An appropriate selection for osteogenic differentiation

Cited by 44 publications

References 123 publications

Facile modification of polycaprolactone nanofibers with egg white protein

Facile modification of polycaprolactone nanofibers with egg white protein

The Role of Adipose Stem Cells in Bone Regeneration and Bone Tissue Engineering

Role of Fzd6 in Regulating the Osteogenic Differentiation of Adipose-derived Stem Cells in Osteoporosis

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