Adipose-derived mesenchymal stem cells and platelet-rich plasma synergistically ameliorate the surgical-induced osteoarthritis in Beagle dogs

Abstract

BackgroundThe purpose of this study is to investigate the clinical effects of platelet-rich plasma (PRP) and adipose-derived mesenchymal stem cell (MSC) as the fundamental treatment of osteoarthritis (OA).MethodsTwenty four Beagle dogs were used as cranial cruciate ligament transection models. The dogs were divided into four groups (n = 6) according to the intra-articular injection materials: the control group with phosphate-buffered saline (PBS), the PRP group with PRP, the MSC group with MSCs emerged in PBS, and the MSC and PRP co-treatment (MP) group with MSCs and PRP.ResultsLameness score, focal compression strength, articular extracellular matrix (ECM) compositions, histopathology, and real-time PCR were used to evaluate the effects of PRP and MSCs on canine OA. In the order of MP, PRP, and MSC group, these all showed positive effects on the evaluated categories. The lameness scores were lower, and the focal compression strengths of the affected femoral articular surface cartilages were higher than those in the OA control group. Also, the inflammatory changes, when evaluated with Mankin scoring and histomorphologic examination, were significantly ameliorated with the treatment of PRP and/or MSCs. The glycosaminoglycan and collagen composition of extracellular matrix was more favorable in the test groups. The ECM-related genes significantly increased through the up-regulation, while the protein expressions of inflammatory cytokines were decreased through the inhibitory effects of PRP and MSCs on chondrocyte apoptosis and inflammatory cytokines.ConclusionsTaken together, this study suggests that PRP and MSCs treatments have a beneficial effect on OA via the stimulation of ECM synthesis and chondrocyte proliferation and via the inhibition of inflammatory reaction.Electronic supplementary materialThe online version of this article (doi:10.1186/s13018-016-0342-9) contains supplementary material, which is available to authorized users.

Mechanisms of Immune Suppression Utilized by Canine Adipose and Bone Marrow-Derived Mesenchymal Stem Cells

“…MSCs have also been widely used for treatment of osteoarthritis in dogs, a progressive degenerative condition that also has an associated inflammatory component. Studies of canine adiposederived MSC (Ad-MSC) and bone marrow-derived MSC (BM-MSC) injected into the elbow and hip joints of dogs with osteoarthritis produced positive results in terms of improvement in clinical measures of osteoarthritis reported in most investigations [44][45][46][47][48][49].…”

The effect of mesenchymal stem cells combined with platelet‐rich plasma on skin wound healing

“…MSCs and PRP improve the degeneration of articular cartilage in the surgically induced osteoarthritis animal model. MSCs and PRP treatment encourage the downregulated expression of TNF‐α compared to the diseased group, and the combined treatment may be beneficial as an inflammatory regulator . The combined effect of MSCs and PRP has been evaluated by Lian et al who found that the wound‐healing rates were significantly higher in the MSCs plus PRP group than in the other groups.…”

Effects of intra-articular injection of mesenchymal stem cells associated with platelet-rich plasma in a rabbit model of osteoarthritis

“…Currently, pharmacological therapies for this disease can only achieve palliation of pain and discomfort (Csaki et al, 2008). Therefore, there is an urgent need for the development of alternative therapies that can fundamentally prevent the destruction of cartilages or stimulate its repair (Yun et al, 2016). In the present study, we investigated the effect of PRP and MSC in the treatment of OA.…”

“…In a previous study, Yun et al (2016) investigated the clinical effects of PRP and MSC in experimental OA, and concluded that combination therapy using both cells has beneficial and synergistic effects on OA via articular extracellular matrix synthesis, chondrocyte proliferation, and anti-inflammatory responses. It was concluded that combination treatment of MSC and PRP may be very useful as an inflammatory regulator for treatment of OA.…”