Adipose-derived Mesenchymal Cells (AMCs): A Promising Future for Skeletal Tissue Engineering

Xu, Yue; Malladi, Preeti; Wagner, Diane R.; Tataria, Monika; Chiou, Michael; Sylvester, Karl G.; Longaker, Michael T.

doi:10.1080/02648725.2006.10648089

Cited by 49 publications

(71 citation statements)

References 96 publications

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“…ASC are readily accessible, easily expandable and have low donor site morbidity; therefore, applying ASC as an alternative cell source for cartilage repair is an area of intense interest [17][18][19]. Most of our current research is focusing on studying the chondrogenic and osteogenic capacities of these cells [11,16,[20][21][22][23]. Because of the low proliferation capability of explanted chondrocytes our group and others are investigating several in vitro chondrogenesis differentiation systems using ASC.…”

Section: Introductionmentioning

confidence: 99%

“…Adipose-derived stromal cells (ASC) are a promising cell source for tissue engineering because of their abundant supply and their capacity for differentiating to bone, cartilage, muscle and fat [12][13][14][15][16]. ASC are readily accessible, easily expandable and have low donor site morbidity; therefore, applying ASC as an alternative cell source for cartilage repair is an area of intense interest [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

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Analysis of the material properties of early chondrogenic differentiated adipose-derived stromal cells (ASC) using an in vitro three-dimensional micromass culture system

Balooch

Chiou

et al. 2007

Biochemical and Biophysical Research Communications

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Cartilage is an avascular tissue with only a limited potential to heal and chondrocytes in vitro have poor proliferative capacity. Recently, adipose-derived stromal cells (ASC) have demonstrated a great potential for application to tissue engineering due to their ability to differentiate into cartilage, bone, and fat. In this study, we have utilized a high density three-dimensional (3D) micromass model system of early chondrogenesis with ASC. The material properties of these micromasses showed a significant increase in dynamic and static elastic modulus during the early chondrogenic differentiation process. These data suggest that the 3D micromass culture system represents an in vitro model of early chondrogenesis with dynamic cell signaling interactions associated with the mechanical properties of chondrocyte differentiation.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analysis of the material properties of early chondrogenic differentiated adipose-derived stromal cells (ASC) using an in vitro three-dimensional micromass culture system

Balooch

Chiou

et al. 2007

Biochemical and Biophysical Research Communications

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“…[1][2][3] However, scientific challenges, immunologic issues, and ethical concerns motivate the examination of reservoirs of stem cells from postnatal tissues. These include, but are not limited to, cells isolated from the bone marrow, 4,5 the circulation or blood vessels, 6,7 adipose tissue, [8][9][10] human placenta, 11 human umbilical cord, 12,13 human amniotic fluid, 14 and skeletal muscle. 15,16 As a means of demonstrating the potential of adult stem cell therapy for regenerative medicine in orthopaedic surgery, the first part of this review will detail current levels of progress being made in research utilizing stem cells isolated from adult skeletal muscle.…”

Section: Introductionmentioning

confidence: 99%

Regenerative medicine in orthopaedic surgery

Corsi

Schwarz

Mooney

et al. 2007

Journal Orthopaedic Research

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Regenerative medicine holds great promise for orthopaedic surgery. As surgeons continue to face challenges regarding the healing of diseased or injured musculoskeletal tissues, regenerative medicine aims to develop novel therapies that will replace, repair, or promote tissue regeneration. This review article will provide an overview of the different research areas involved in regenerative medicine, such as stem cells, bioinductive factors, and scaffolds. The potential use of stem cells for orthopaedic tissue engineering will be addressed by presenting the current progress with skeletal muscle-derived stem cells. As well, the development of a revascularized massive allograft will be described and will serve as a prototypic model of orthopaedic tissue engineering. Lastly, we will describe current approaches used to design cell instructive materials and how they can be used to promote and regulate the formation of bony tissue. ß

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“…Furthermore, stem cells secrete bioactive factors that suppress apoptosis, enhance angiogenesis, and stimulate mitosis and differentiation of tissue intrinsic reparative cascades (Caplan and Dennis, 2006). These effects may lead to reactivation and augmentation of the fracture healing mechanism (Xu et al, 2005). Another reason for choosing ADSCs was that a high number of stem cells were needed.…”

Section: Discussionmentioning

confidence: 99%

“…ADSCs are multipotent progenitor cells, capable of differentiating into a number of mesodermal lineages including osteoblasts (Xu et al, 2005;Moseley et al, 2006). A number of experiments have demonstrated the efficacy of ADSCs in enhancing osteogenesis (Xu et al, 2005;Bunnell et al, 2008). Furthermore, stem cells secrete bioactive factors that suppress apoptosis, enhance angiogenesis, and stimulate mitosis and differentiation of tissue intrinsic reparative cascades (Caplan and Dennis, 2006).…”

Section: Discussionmentioning

confidence: 99%

Augmentation of bone healing of nonunion fracture using stem cell based tissue engineering in a dog: a case report

Lee¹,

Chung²,

Heo³

et al. 2009

Vet. Med. - Czech

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ABSTRACT:A 4-year-old, intact male crossbreed dog, weighing 27 kg, was referred for the treatment of a nonunion fracture. The radiographs revealed displacement of the radius and ulna bone fracture fragment and a sclerotic fracture end of the radius. Autologous adipose derived stem cells (ADSCs) were isolated and expanded ex vivo in a culture. The ADSCs (3.2 × 107 cells) were seeded on a composition scaffold made from hydroxyapatite (HA) and chitosan (CH) fibers. The seeded scaffold with ADSCs was placed on the fracture site and the bone fracture was stabilized. A sample of seeded scaffold with ADSCs was taken to evaluate the extent of cell attachment and morphology on the scaffold using scanning electron microscopy (SEM). SEM showed that ADSCs adhered to the scaffold well and many bone nodules formed from the bone matrix secreted by ADSCs. Three months after surgery, the nonunion had successfully healed with no complications. The application of a composition scaffold of HA and CH containing ADSCs can be used to treat a nonunion fracture by augmenting bone healing and may decrease the risk of surgical failure of nonunion fractures.

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Adipose-derived Mesenchymal Cells (AMCs): A Promising Future for Skeletal Tissue Engineering

Cited by 49 publications

References 96 publications

Analysis of the material properties of early chondrogenic differentiated adipose-derived stromal cells (ASC) using an in vitro three-dimensional micromass culture system

Analysis of the material properties of early chondrogenic differentiated adipose-derived stromal cells (ASC) using an in vitro three-dimensional micromass culture system

Regenerative medicine in orthopaedic surgery

Augmentation of bone healing of nonunion fracture using stem cell based tissue engineering in a dog: a case report

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