Adiponectin promotes human jaw bone marrow mesenchymal stem cell chemotaxis via CXCL1 and CXCL8

Abstract

Adiponectin (APN) is known to promote the osteogenic differentiation of human jaw bone marrow mesenchymal stem cells (h‐JBMMSCs). However, the underlying mechanism has not been fully elucidated. Previously, we showed that APN could promote h‐JBMMSC osteogenesis via APPL1‐p38 by up‐regulating osteogenesis‐related genes. Here, we aimed to determine whether APN could promote h‐JBMMSC chemotaxis through CXCL1/CXCL8. The CCK‐8, wound healing and transwell assays were used to evaluate the proliferation, migration and chemotaxis of h‐JBMMSCs with or without APN treatment. Chemotaxis‐related genes were screened using RNA‐seq, and the results were validated using real‐time PCR and ELISA. We also performed Western blot using the AMPK inhibitor, WZ4003, and the p38 MAPK inhibitor, SB203580, to identify the signalling pathway involved. We found that APN could promote h‐JBMMSC chemotaxis in the co‐culture transwell system. CXCL1 and CXCL8 were screened and confirmed as the up‐regulated target genes. The APN‐induced CXCL1/8 up‐regulation to promote chemotaxis could be blocked by CXCR2 inhibitor SB225002. Western blot revealed that the phosphorylation of AMPK and p38 MAPK increased in a time‐dependent manner with APN treatment. Additionally, WZ4003 and SB203580 could suppress the APN‐induced overexpression of CXCL1 and CXCL8. The results of the transwell chemotaxis assay also supported the above results. Our data suggest that APN can promote h‐JBMMSC chemotaxis by up‐regulating CXCL1 and CXCL8.

Adiponectin and its receptors are involved in hypertensive vascular injury

“…Previous studies have demonstrated that APN could attenuate lipid deposition and apoptosis through activating p38 MAPK pathway ( 37 , 38 ). In the present study, it was demonstrated that APN alone could induce p38 phosphorylation, which is in accordance with previous studies ( 39 , 40 ). Furthermore, Ang II promoted p38 phosphorylation.…”

CRYAB promotes osteogenic differentiation of human bone marrow stem cells via stabilizing β‐catenin and promoting the Wnt signalling

“…As determined by qRT‐PCR analysis, the expression of these genes was consistent with the dataset (Figure B and C). Previous studies have reported several of the above‐mentioned genes (CIDEC, MAOA, SAA1, SVEP1, FBLN2, ADARB1, ZBTB16, ANGPT1, FBLN1, FZD4, OMD, ABI3BP, RGS4, CXCL8) are closely related to bone homeostasis and the process of osteogenic differentiation, and these studies further supported the reliability of the dataset. To identify the BMSCs osteogenic differentiation‐related genes, we chose 10 of the other upregulated genes, namely EFHD1, FKBP5, APOD, ACTC1, SORT1, COMP, CLEC3B, CRYAB, WASF3, LMOD1, and downregulated their expression by specific siRNA in BMSCs (Figure D‐M).…”

Gene Expression Regulation and Secretory Activity of Mesenchymal Stem Cells upon In Vitro Contact with Microarc Calcium Phosphate Coating

“…The chemokine GROα (CXCL1), a mediator of MSC chemotaxis [ 64 ], apparently contributes to the formation of a network of inflammatory cytokines/chemokines and one of the hematopoietic stem cell (HSCs) growth factors (SCGF-b) ( Figure 2 ). CXCL1, on the other hand, can induce osteoclastogenic activity [ 65 ].…”