Adhesion of inactivated probiotic strains to intestinal mucus

Ouwehand, Arthur C.; Tölkkö, Satu; Kulmala, Jarmo; Salminen, Seppo; Salminen, Eeva

doi:10.1046/j.1472-765x.2000.00773.x

Cited by 115 publications

(96 citation statements)

References 19 publications

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“…Both probiotic strains studied in this work displayed similar coaggregation with the pathogen C. jejuni CIP 70.2 T , regardless of whether they had been heattreated. Inactivation by heat has been shown previously to generally decrease the adhesion of probiotic bacteria (Ouwehand et al, 2000) but a similar improvement of adhesion to that described in our study has also been demonstrated for a strain of Propionibacterium freudenreichii (Ouwehand et al, 2000). The reason why adhesion is improved after heat treatment has not yet been elucidated but it may be due to the nature of the molecules involved.…”

Section: Discussionsupporting

confidence: 88%

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In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with foodborne zoonotic bacteria, especially Campylobacter jejuni

Tareb

Bernardeau²,

Guéguen

et al. 2013

Journal of Medical Microbiology

112

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Bacterial aggregation and/or adhesion are key factors for colonization of the digestive ecosystem and the ability of probiotic strains to exclude pathogens. In the present study, two probiotic strains, Lactobacillus rhamnosus CNCM-I-3698 and Lactobacillus farciminis CNCM-I-3699, were evaluated as viable or heat-killed forms and compared with probiotic reference Lactobacillus strains (Lb. rhamnosus GG and Lb. farciminis CIP 103136). The autoaggregation potential of both forms was higher than that of reference strains and twice that of pathogenic strains. The coaggregation potential of these two beneficial micro-organisms was evaluated against several pathogenic agents that threaten the global safety of the feed/food chain: Escherichia coli, Salmonella spp., Campylobacter spp. and Listeria monocytogenes. The strongest coaggregative interactions were demonstrated with Campylobacter spp. by a coaggregation test, confirmed by electron microscopic examination for the two forms. Viable forms were investigated for the nature of the bacterial cell-surface molecules involved, by sugar reversal tests and chemical and enzymic pretreatments. The results suggest that the coaggregation between both probiotic strains and C. jejuni CIP 70.2 T is mediated by a carbohydrate-lectin interaction. The autoaggregation potential of the two probiotics decreased upon exposure to proteinase, SDS or LiCl, showing that proteinaceous components on the surface of the two lactobacilli play an important role in this interaction. Adhesion abilities of both Lactobacillus strains were also demonstrated at significant levels on Caco-2 cells, mucin and extracellular matrix material. Both viable and heat-killed forms of the two probiotic lactobacilli inhibited the attachment of C. jejuni CIP 70.2 T to mucin. In conclusion, in vitro assays showed that Lb. rhamnosus CNCM-I-3698 and Lb. farciminis CNCM-I-3699, as viable or heat-killed forms, are adherent to different intestinal matrix models and are highly aggregative in vitro with pathogens, especially Campylobacter spp., the most commonly reported zoonotic agent in the European Union. This study supports the need for further in vivo investigations to demonstrate the potential food safety benefits of Lb. rhamnosus CNCM-I-3698 and Lb. farciminis CNCM-I-3699, live or heat-killed, in the global feed/food chain.

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Section: Discussionsupporting

confidence: 88%

“…It was observed for example that there was a dramatic decrease in the adhesive ability of Lb. rhamnosus GG whatever the intestinal matrix model (Tuomola et al, 1999) or on Caco-2 cells (Ouwehand et al, 2000). The reduction of adhesion can be explained by the heatsensitive proteinaceous nature of the molecules involved in Lb.…”

Section: Discussionmentioning

confidence: 99%

In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with foodborne zoonotic bacteria, especially Campylobacter jejuni

Tareb

Bernardeau²,

Guéguen

et al. 2013

Journal of Medical Microbiology

112

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“…Cell surface hydrophobicity was determined according to the capacity of the four selected strains and the five indicator strains to individually partition into xylene from PBS (Ouwehand et al 1999). The cells were washed twice with PBS and the optical density (A) at 450 nm adjusted to 0.5 ± 0.01.…”

Section: Cell Surface Hydrophobicitymentioning

confidence: 99%

“…Selection of potential probiotic strains is based on many different criteria, such as acid and bile tolerance (tolerance to gastrointestinal environment), antagonistic activity against pathogens and survival in gastric juice. Adhesion of probiotic microorganism to the intestinal mucosa is considered important for many of the observed probiotic health effects (Ouwehand et al 1999). Adhesion is regarded a prerequisite for colonization in the fishes intestinal tract, antagonistic activity against enteropathogens, modulation of the immune system and for increased healing of the damaged gastric mucosa (Rinkinen et al 2000).…”

Section: Introductionmentioning

confidence: 99%

In vitro evaluation of adhesion and aggregation abilities of four potential probiotic strains isolated from guppy (Poecilia reticulata)

Balakrishna¹

2013

Braz. arch. biol. technol.

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The aim of this work was to study the probiotic-related characteristics of four strains of bacteria isolated from the normal flora of the guppy, Poecilia reticulata. In vitro results showed that the strains, namely, MBTU_PB1, MBTU_PB2, MBTU_PB3 and MBTU_PB4 had higher adhesion abilities than the tested indicator strains. However, an association between the cell-surface hydrophobicity and the ability to adhere to the intestinal mucus was not observed for these strains. Further, the selected strains were strongly autoaggregating (autoaggregation percentage ≥ 80) and also showed strain-specific coaggregation abilities with the tested indicator strains.

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“…This process is thought to enhance mucosal damage and intestinal inflammation related to gut RT (5)(6)(7)(8).…”

mentioning

confidence: 99%

MMP7 Modulation by Short- and Long-term Radiotherapy in Patients with Rectal Cancer

Stene

Polistena

Gaber

et al. 2018

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Adhesion of inactivated probiotic strains to intestinal mucus

Cited by 115 publications

References 19 publications

In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with foodborne zoonotic bacteria, especially Campylobacter jejuni

In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with foodborne zoonotic bacteria, especially Campylobacter jejuni

In vitro evaluation of adhesion and aggregation abilities of four potential probiotic strains isolated from guppy (Poecilia reticulata)

MMP7 Modulation by Short- and Long-term Radiotherapy in Patients with Rectal Cancer

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