Adhesion of a Monolayer of Fibroblast Cells to Fibronectin under Sonic Vibrations in a Bioreactor

Titze, Ingo R.; Klemuk, Sarah A.; Lü, Xiaoying

doi:10.1177/000348941212100602

Cited by 7 publications

(6 citation statements)

References 29 publications

(39 reference statements)

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Section: Introductionmentioning

confidence: 99%

“…In vitro studies have shown that local vibrations and fluid agitation that generate shear stresses can influence the adhesion and proliferation of cells [45][46][47] . Above a specific oscillating stress limit, human fibroblasts fail to adhere to the substrate 45 . Additionally, in vitro studies have also shown that vibrations negatively influence the proliferation of certain types of mouse embryonic fibroblasts 46 , with vibration amplitudes measuring approximately 100 nm at frequencies of 100 Hz and 1 kHz being tested.…”

Section: Introductionmentioning

confidence: 99%

“…The actuator could be either embedded within the GDD or affixed to it prior to surgical implantation. The shear stresses generated directly by the vibration of the actuator and indirectly by the fluid agitation resulting from this vibration would reduce the adhesion of fibroblasts and endothelial cells [45][46][47] . As a result, the actuation would impede complete encapsulation by preventing cells from adhering to large portions of the implant.…”

Section: Introductionmentioning

confidence: 99%

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Resonant magnetoelastic microstructures for wireless actuation of liquid flow on 3D surfaces and use in glaucoma drainage implants

2015

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Magnetoelastic resonators made from metal alloy foils are widely used for miniature wireless anti-theft tags and have also been explored for use in various sensing applications. Through annealing within three-dimensional (3D) molds, these foils can be formed into curved structures. Consequently, magnetoelastic materials present an opportunity for the development of a new class of wireless, actuators that have small form factors and low surface profiles and that can conform to curved surfaces. This paper describes passive, wireless, resonant magnetoelastic actuators intended for the generation of fluid flow on the surfaces of implantable Ahmed glaucoma drainage devices. The actuators are remotely excited to resonance using a magnetic field generated by external coils. The fluid flow is intended to limit cellular adhesion to the surface of the implant, as this adhesion can ultimately lead to implant encapsulation and failure. The actuators are micromachined from planar 29-μm-thick foils of Metglas 2826MB (Fe 40 Ni 38 Mo 4 B 18), an amorphous magnetoelastic alloy, using photochemical machining. Measuring 10.3 Â 5.6 mm 2 , the planar structures are annealed in 3D molds to conform to the surface of the drainage device, which has an aspherical curvature. Six actuator designs are described, with varying shapes and resonant mode shapes. The resonant frequencies for the different designs vary from 520 Hz to 4.7 kHz. Flow velocities of up to 266 μm s −1 are recorded at a wireless activation range of 25-30 mm, with peak actuator vibration amplitudes of 1.5 μm. Integrated actuators such as those described here have the potential to greatly enhance the effectiveness of glaucoma drainage devices at lowering eye pressure and may also be useful in other areas of medicine.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Resonant magnetoelastic microstructures for wireless actuation of liquid flow on 3D surfaces and use in glaucoma drainage implants

2015

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“…A device with the potential to satisfy these requirements is a custom-modified torsional rheometer-bioreactor (TRB). The TRB is a commercial rheometer (Gemini model, Malvern Instruments, LTD) with appliances to support tissue and cell viability [11][12][13]. Initial feasibility of the rheometer to deliver shear stresses at low audio frequencies with parallel plate appliances was shown by adjusting sample thickness, plate radius, material viscoelasticity, and motor inertia [11].…”

Section: Introductionmentioning

confidence: 99%

“…A third configuration, a glass coverslip mounted to the cup or the moving parallel plate of the TRB, delivered shear stresses up to 2200 Pa at 100 Hz to a monolayer of cells. This delivery was under controlled temperature, humidity, and CO 2 conditions using a custom incubator fitted to the commercial rheometer [13].…”

Section: Introductionmentioning

confidence: 99%

A Multiwell Disc Appliance Used to Deliver Quantifiable Accelerations and Shear Stresses at Sonic Frequencies

et al. 2014

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To mimic in vivo vibration of vocal fold cells, we studied the controllability and range of frequency, acceleration, duration, and shear stress in a new bioreactor attachment. The custom multiwell disc appliance fits into a commercially built rheometer, together termed a torsional rheometer bioreactor (TRB). Previous attachments to the TRB were capable of 50-100 Hz vibrations at relatively high strains but were limited to single-sample experiments. The TRB-multiwell disc system accommodates 20 samples in partially fluid-filled wells in an aseptic environment delivering three different acceleration conditions to different samples simultaneously. Frequency and amplitude used to calculate acceleration along with duration and shear stress were controllable and quantifiable using a combination of built-in rheometer sensors, manufacturer software, and smooth particle hydrodynamics (SPH) simulations. Computed shear stresses at the well bottom using SPH in two and three dimensions were verified with analytical approximations. Results demonstrate capabilities of the TRB-multiwell disc system that, when combined with computational modeling, provide quantifiable vibration parameters covering frequencies 0.01-250 Hz, accelerations of 0.02-300 m/s 2 , and shear stresses of 0.01-1.4 Pa. It is

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Measuring the adhesion limit of fibronectin for fibroblasts with a narrow-gap rotational rheometer

Dakhil

Xuan

Hübner

et al. 2017

Bioprocess Biosyst Eng

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We study the adhesion limit of 3T6 fibroblasts, cultured in Dulbecco's modified Eagle's medium with 10% fetal bovine serum at 37 °C and 5% CO, with a narrow-gap rotational rheometer in the parallel-disk configuration. Reducing the uncertainty in gap width to about 1 µm allows studying the cells at narrow gaps, which enables to study the critical shear stress of the cells in low-viscous media. The adhesion limit on fibronectin-coated glass plates is determined as a function of concentration and adhesion time. We found that cells in groups have a tendency to detach at slightly higher shear stresses than single cells. Moreover, 60 min after the settling phase are enough for the cells to adhere to the coated plate at maximum strength. We show that the setup may also be used for cells that are not adhered from suspension, but are grown directly on the substrate.

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Adhesion of a Monolayer of Fibroblast Cells to Fibronectin under Sonic Vibrations in a Bioreactor

Cited by 7 publications

References 29 publications

Resonant magnetoelastic microstructures for wireless actuation of liquid flow on 3D surfaces and use in glaucoma drainage implants

Resonant magnetoelastic microstructures for wireless actuation of liquid flow on 3D surfaces and use in glaucoma drainage implants

A Multiwell Disc Appliance Used to Deliver Quantifiable Accelerations and Shear Stresses at Sonic Frequencies

Measuring the adhesion limit of fibronectin for fibroblasts with a narrow-gap rotational rheometer

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