Adhesion characteristics of lactobacillus is a criterion of the probiotic choice

Кравцов, Э. Г.; Yermolayev, A. V.; Anokhina, I. V.; Yashina, N. V.; Chesnokova, V. L.; Далин, М. В.

doi:10.1007/s10517-008-0058-x

Cited by 34 publications

(23 citation statements)

References 5 publications

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“…Previous studies with highly adherent strains such as B. lactis Bb12 and L. rhamnosus GG had shown prevention of diarrhea [36]. The biofilm formed by the probiotic while adhering to epithelial cells would inhibit the colonization of foreign, pathogenic microbiota [20,21]. In this study, cell surface adherence was evaluated by two methods.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Putative Probiotic Bacterial Strain, Bacillus amyloliquefaciens, from North East Himalayan Soil Based on In Vitro and In Vivo Functional Properties

Ibrahim¹,

Babu²,

Pandikumar³

et al. 2011

Probiotics & Antimicro. Prot.

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This study is aimed to isolate some novel probiotics from the soils of North East Himalayas. Eleven Gram positive isolates were obtained in MRS with Oxgall media from soil samples. Four of the isolates withstood the in vitro gastric juice pH 3.0 and 0.45% bile salt tolerance screening. Among these, PBT 3 showed high cell surface hydrophobicity and adhered to the Caco-2 cells. 16s rDNA gene sequences of this probiotic strains were identified as Bacillus amyloliquefaciens (accession no: JF836079). In in vivo bioefficacy evaluation by DSS-induced colitis animals, B. amyloliquefaciens significantly ameliorated the loss in body weight. Further, the treatment altered the levels of myeloperoxidase, lipoperoxides, and mucous content in the colon tissues compared with normal colon. It reduced the protein and mRNA levels of pro-inflammatory cytokines such as TNF-α and IL-1β. These biochemical findings were supported by histopathological evidences. Our study reports the use of B. amyloliquefaciens isolated from Himalayan soil as probiotic and its beneficial effect on IBD for the first time and suggests that this could be used as potential probiotics in functional foods or as a curative agent.

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Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Putative Probiotic Bacterial Strain, Bacillus amyloliquefaciens, from North East Himalayan Soil Based on In Vitro and In Vivo Functional Properties

Ibrahim¹,

Babu²,

Pandikumar³

et al. 2011

Probiotics & Antimicro. Prot.

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“…These bacteria have the ability to adhere to the mucosal layers and epithelium, promoting colonization (43,44). Through interaction with the intestinal epithelial cells, lactobacilli can cause immunomodulation and offer protection to the intestinal barrier by antagonistic activities against pathogens ( [44][45][46].…”

Section: Discussionmentioning

confidence: 99%

Effects of Xylo-Oligosaccharides on Broiler Chicken Performance and Microbiota

Maesschalck

Eeckhaut

Maertens³

et al. 2015

Appl Environ Microbiol

189

153

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hIn broiler chickens, feed additives, including prebiotics, are widely used to improve gut health and to stimulate performance. Xylo-oligosaccharides (XOS) are hydrolytic degradation products of arabinoxylans that can be fermented by the gut microbiota. In the current study, we aimed to analyze the prebiotic properties of XOS when added to the broiler diet. Administration of XOS to chickens, in addition to a wheat-rye-based diet, significantly improved the feed conversion ratio. XOS significantly increased villus length in the ileum. It also significantly increased numbers of lactobacilli in the colon and Clostridium cluster XIVa in the ceca. Moreover, the number of gene copies encoding the key bacterial enzyme for butyrate production, butyryl-coenzyme A (butyryl-CoA):acetate CoA transferase, was significantly increased in the ceca of chickens administered XOS. In this group of chickens, at the species level, Lactobacillus crispatus and Anaerostipes butyraticus were significantly increased in abundance in the colon and cecum, respectively. In vitro fermentation of XOS revealed cross-feeding between L. crispatus and A. butyraticus. Lactate, produced by L. crispatus during XOS fermentation, was utilized by the butyrate-producing Anaerostipes species. These data show the beneficial effects of XOS on broiler performance when added to the feed, which potentially can be explained by stimulation of butyrate-producing bacteria through cross-feeding of lactate and subsequent effects of butyrate on gastrointestinal function. Cereal fibers are composed of carbohydrate polymers that are resistant to digestion in the small intestines of monogastric animals but are completely or partially fermented in the distal gut, and they are believed to stimulate gut health (1). The main components of the cereal fiber fraction are arabinoxylans (AX), pectins, resistant starch, cellulose, ␤-glucans, and lignin (2). Hydrolytic degradation of the heteropolymer AX results in a mixture of arabinose-substituted xylo-oligosaccharides (arabinoxylan-oligosaccharides) (AXOS) and nonsubstituted xylo-oligosaccharides (XOS) (3). XOS are oligomers consisting of xylose units linked through ␤-(1-4) linkages (4). Selective fermentation of XOS has been shown to induce changes in both the composition and activity of the gastrointestinal microbiota, improving the health and well-being of the host. This suggests that XOS could fulfill the definition of a prebiotic (5). The production of lactate and shortchain fatty acids (SCFA), including butyrate, upon fermentation of XOS, has been confirmed in several in vitro and in vivo studies (3, 6). Lactate can stimulate butyrate production due to crossfeeding between lactate-producing bacteria and lactate-utilizing butyrate-producing bacteria from Clostridium cluster XIVa (7). Butyrate has proven beneficial effects on gastrointestinal function, since it has anti-inflammatory properties, fuels epithelial cells, and increases the intestinal epithelial integrity. In addition, butyrate has been shown to improve growth pe...

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“…Probiotics also stabilize cellular cytoskeleton and gut barrier integrity to prevent epithelial invasion [80]. Some Bifidobacteria and Lactobacilli prevent adhesion of pathogenic bacteria by secreting lectin-like bacteriocins [81]. …”

Section: Gastrointestinal Mucosal Barriermentioning

confidence: 99%

Protection and Restitution of Gut Barrier by Probiotics: Nutritional and Clinical Implications

Rao¹,

Samak²

2013

CNF

200

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Probiotics are beneficial bacteria present in various dietary components and many of these colonize in the human and animal intestine. In the gut probiotics help the host by assisting in maintenance of normal mucosal homeostasis. Probiotics not only help maintain normal function of the gut mucosa, but also protect mucosa from injurious factors such as toxins, allergens and pathogens. The beneficial effect of probiotics is mediated by multiple mechanisms, including cytoprotection, cell proliferation, cell migration, resistance to apoptosis, synthesis of proteins and gene expression. One of the important cytoprotective effects of probiotics in the intestinal mucosa is to strengthen the epithelial tight junctions and preservation of mucosal barrier function. Probiotics not only enhance barrier function by inducing synthesis and assembly of tight junction proteins, but also preventing disruption of tight junctions by injurious factors. Bioactive factors released by probiotics trigger activation of various cell signaling pathways that lead to strengthening of tight junctions and the barrier function. This article reviews and summarizes the current understanding of various probiotics that are involved in the protection of gut barrier function, highlights the cellular and molecular mechanisms involved in the protective effect and addresses the clinical implications of probiotic supplementation.

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Adhesion characteristics of lactobacillus is a criterion of the probiotic choice

Cited by 34 publications

References 5 publications

Isolation and Characterization of Putative Probiotic Bacterial Strain, Bacillus amyloliquefaciens, from North East Himalayan Soil Based on In Vitro and In Vivo Functional Properties

Isolation and Characterization of Putative Probiotic Bacterial Strain, Bacillus amyloliquefaciens, from North East Himalayan Soil Based on In Vitro and In Vivo Functional Properties

Effects of Xylo-Oligosaccharides on Broiler Chicken Performance and Microbiota

Protection and Restitution of Gut Barrier by Probiotics: Nutritional and Clinical Implications

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