Isolation and Characterization of Putative Probiotic Bacterial Strain, Bacillus amyloliquefaciens, from North East Himalayan Soil Based on In Vitro and In Vivo Functional Properties

Abstract

This study is aimed to isolate some novel probiotics from the soils of North East Himalayas. Eleven Gram positive isolates were obtained in MRS with Oxgall media from soil samples. Four of the isolates withstood the in vitro gastric juice pH 3.0 and 0.45% bile salt tolerance screening. Among these, PBT 3 showed high cell surface hydrophobicity and adhered to the Caco-2 cells. 16s rDNA gene sequences of this probiotic strains were identified as Bacillus amyloliquefaciens (accession no: JF836079). In in vivo bioefficacy evaluation by DSS-induced colitis animals, B. amyloliquefaciens significantly ameliorated the loss in body weight. Further, the treatment altered the levels of myeloperoxidase, lipoperoxides, and mucous content in the colon tissues compared with normal colon. It reduced the protein and mRNA levels of pro-inflammatory cytokines such as TNF-α and IL-1β. These biochemical findings were supported by histopathological evidences. Our study reports the use of B. amyloliquefaciens isolated from Himalayan soil as probiotic and its beneficial effect on IBD for the first time and suggests that this could be used as potential probiotics in functional foods or as a curative agent.

Effects of dietary Bacillus amyloliquefaciens supplementation on growth performance, intestinal morphology, inflammatory response, and microbiota of intra-uterine growth retarded weanling piglets

“…Probiotic bacteria can regulate lymphocyte cytokine production and inflammatory responses [ 60 ]. Hairul Islam et al [ 61 ] reported that BA could alleviate increased concentrations of pro-inflammatory mediators, such as TNF-α and IL-1β in a mouse model of colitis induced by dextran sulfate. The results of the present study further corroborate the ability of BA to temper IUGR-induced excessive secretion of TNF-α in the small intestine, which may be due to the simultaneous increase in IL-10 content after administration of BA.…”

Sodium salt medium-chain fatty acids andBacillus-based probiotic strategies to improve growth and intestinal health of gilthead sea bream (Sparus aurata)

“…This probiotic ameliorated the damage caused by inflammation in dextran sulphate sodium (DSS)-induced colitis in mice ( Hairul Islam et al, 2011 ) and LPS-induced stress in broilers ( Li et al, 2015 ) by down-regulating expression of pro-inflammatory cytokines such as TNF α , IL1 β and IL2 and up-regulating the anti-inflammatory cytokine IL10 upon exacerbated inflammation. In addition, a decrease in neutrophil myeloperoxidase and an increase in antioxidant molecules (superoxide dismutase and catalase) was observed ( Hairul Islam et al, 2011 ). In the present study, superoxide dismutase and catalase were not measured, but the observed decrease in oxidative radicals measured with the respiratory burst assay indirectly reflects this anti-oxidant effect.…”

Isolation and characterization of a Bacillus amyloliquefaciens strain with zearalenone removal ability and its probiotic potential

“…For example, Islam et al isolated a B . amyloliquefaciens strain from soil, and demonstrated it had a beneficial effect on inflammatory bowel disease [ 23 ]. Larsen et al reported a B .…”