Effects of dietary Bacillus amyloliquefaciens supplementation on growth performance, intestinal morphology, inflammatory response, and microbiota of intra-uterine growth retarded weanling piglets

Li, Yue; Zhang, Hao; Su, W.P. Daniel; Ying, Zhixiong; Chen, Yueping; Zhang, Lili; Lu, Zhaoxin

doi:10.1186/s40104-018-0236-2

Cited by 52 publications

(49 citation statements)

References 83 publications

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“…Previous study demonstrated that the impaired intestinal morphology may be associated with the imbalance of cell apoptosis and proliferation [26]. In the current study, IUGR piglets enhanced the AI and reduced the PI of enterocytes when compared with NBW weaned piglets.…”

Section: Discussionsupporting

confidence: 58%

Dietary Dihydroartemisinin Supplementation Alleviates Intestinal Inflammatory Injury Through TLR4/NODs/NF-κB Signaling Pathway in Weaned Piglets with Intrauterine Growth Retardation

Zhao

et al. 2020

Preprint

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Background: Intrauterine growth retardation (IUGR) leads to impaired intestinal morphology and function. IUGR infants are at a risk for intestinal inflammatory diseases. Dihydroartemisinin (DHA) is a derivative of artemisinin, which possesses anti-inflammatory activity and immunomodulatory effect. However, little is known about the effects of DHA on IUGR piglets. Therefore, the present study was conducted to investigate whether dietary DHA supplementation could attenuate intestinal injury in IUGR weaned piglets. Methods: Piglets with normal birth weight or IUGR were fed the basal diet or basal diet supplemented with 80 mg/kg DHA from 21 d to 49 days of age. At 49 days of age, eight piglets from each group with nearly similar body weight were sacrificed. The jejunal and ileal samples were collected for further analysis.Results: IUGR impaired intestinal morphology, increased intestinal inflammatory response, raised enterocyte apoptosis and reduced enterocyte proliferation and activated TLR4/NODs/NF-κB signaling pathway. DHA supplementation improved intestinal morphology, indicated by higher villus height, villus height to crypt depth ratio, villus surface area and lower villus width of IUGR piglets (P < 0.05). DHA inclusion exhibited higher apoptosis index and the expression of caspase-3, and lower proliferation index and the expression of proliferating cell nuclear antigen in the intestine of IUGR piglets (P < 0.05). Diet supplemented with DHA could attenuate intesitnal inflammation, indicated by higher concentrations of intestinal inflammatory cytokines and lipopolysaccharides in IUGR piglets (P < 0.05). In addition, DHA down-regulated the related mRNA expressions of TLR4/NODs/NF-κB signaling pathway and up-regulated mRNA expressions of TLR4 and NODs signaling negative regulators in the intestine of IUGR piglets (P < 0.05). Dietary DHA supplementation decreased the protein expressions of toll-like receptors 4, phosphorylated NF-κB (pNF-κB) inhibitor α, nuclear pNF-κB, and increased the protein expression of cytoplasmic pNF-κB in the intestine of IUGR piglets (P < 0.05). Conclusions: Dietary DHA supplementation may have beneficial effects in improving intestinal morphology, regulating enterocyte proliferation and apoptosis, and alleviating intestinal inflammation through TLR4/NODs/NF-κB signaling pathway in IUGR weaned piglets.

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Section: Discussionsupporting

confidence: 58%

Dietary Dihydroartemisinin Supplementation Alleviates Intestinal Inflammatory Injury Through TLR4/NODs/NF-κB Signaling Pathway in Weaned Piglets with Intrauterine Growth Retardation

Zhao

et al. 2020

Preprint

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“…The FSBM contained more probiotics and organic acids compared with SBM. It has been reported that probiotics and organic acids could regulate the production of cytokines and relieve ETEC K88-induced inflammation by suppressing the ETEC K88 proliferation and decreasing enterotoxin release [20][21][22][23]. This finding was also confirmed by the results of high-throughput sequencing, which shown that FSBM decreased the population of inflammation-related bacteria.…”

Section: Discussionmentioning

confidence: 56%

Dietary fermented soybean meal replacement alleviates diarrhea in weaned piglets challenged with enterotoxigenic Escherichia coli K88 by modulating inflammatory cytokine levels and cecal microbiota composition

Wang

Hao

et al. 2020

BMC Vet Res

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Background: Impaired gut microbiota leads to pathogenic bacteria infection, pro-inflammatory response and postweaning diarrhea. Enterotoxigenic Escherichia coli (ETEC) K88 is a major cause of post-weaning diarrhea in weaned piglets. Fermented soybean meal (FSBM) could relieve diarrhea, alleviate inflammatory response, and modulate gut microbiota of weaned piglets. We used ETEC K88-challenged weaned piglet model to investigate the effects of FSBM on the growth performance, inflammatory response and cecal microbiota. Twenty-four crossbred piglets (6.8 ± 0.5 kg; 21 ± 2 days of age) were allotted into 2 treatment fed the diets with or without FSBM (6% at the expense of soybean meal). Six weaned piglets in each diet treatment were challenged by ETEC K88 (1 × 10 9 CFU/ piglets) on day 15. The experimental period lasted for 20 days.

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“…Thin sections (5 μm) from the jejunum were taken using a one-step terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) kit (#A113-03; Vazyme Biotech Co., Ltd., Nanjing, Jiangsu, China) to determine the apoptotic rate [32]. Each tissue section was permeabilized with a proteinase K solution (20 μg/mL) at room temperature for 20 min.…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…In each slice, 15 random crypts were measured by an assessor blinded regarding the source of the samples. The cell proliferation index was calculated by dividing the number of Ki67-positive cells by the total number of crypt cells, based on the methods of previous studies [32,35].…”

Section: Immunohistochemistrymentioning

confidence: 99%

Comparison of the protective effects of resveratrol and pterostilbene against intestinal damage and redox imbalance in weanling piglets

Zhang

Chen

et al. 2020

J Animal Sci Biotechnol

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Background: Evidence indicates that early weaning predisposes piglets to intestinal oxidative stress and increases the risk of intestinal dysfunction; however, there are minimal satisfactory treatment strategies for these conditions. This study investigated the potential of resveratrol and its analog, pterostilbene, as antioxidant protectants for regulating intestinal morphology, barrier function, and redox status among weanling piglets. Methods: A total of 144 piglets were selected at 21 days of age and randomly allocated into one of four treatment groups, each of which included six replicates. Piglets in a sow-reared control group were suckling normally between ages 21 and 28 days, while those in weaned groups were fed a basal diet, supplemented with either 300 mg/kg of resveratrol or with 300 mg/kg of pterostilbene. Parameters associated with intestinal injury and redox status were analyzed at the end of the feeding trial. Results: Early weaning disrupted the intestinal function of young piglets, with evidence of increased diamine oxidase activity and D-lactate content in the plasma, shorter villi, an imbalance between cell proliferation and apoptosis, an impaired antioxidant defense system, and severe oxidative damage in the jejunum relative to suckling piglets. Feeding piglets with a resveratrol-supplemented diet partially increased villus height (P = 0.056) and tended to diminish apoptotic cell numbers (P = 0.084) in the jejunum compared with those fed a basal diet. Similarly, these beneficial effects were observed in the pterostilbene-fed piglets. Pterostilbene improved the feed efficiency of weanling piglets between the ages of 21 and 28 days; it also resulted in diminished plasma diamine oxidase activity and D-lactate content relative to untreated weaned piglets (P < 0.05). Notably, pterostilbene restored jejunal antioxidant capacity, an effect that was nearly absent in the resveratrol-fed piglets. Pterostilbene reduced the malondialdehyde and 8-hydroxy-2´-deoxyguanosine contents of jejunal mucosa possibly through its regulatory role in facilitating the nuclear translocation of nuclear factor erythroid-2-related factor 2 and the expression levels of NAD(P)H quinone dehydrogenase 1 and superoxide dismutase 2 (P < 0.05). Conclusions: The results indicate that pterostilbene may be more effective than its parent compound in alleviating early weaning-induced intestinal damage and redox imbalance among young piglets.

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Effects of dietary Bacillus amyloliquefaciens supplementation on growth performance, intestinal morphology, inflammatory response, and microbiota of intra-uterine growth retarded weanling piglets

Cited by 52 publications

References 83 publications

Dietary Dihydroartemisinin Supplementation Alleviates Intestinal Inflammatory Injury Through TLR4/NODs/NF-κB Signaling Pathway in Weaned Piglets with Intrauterine Growth Retardation

Dietary Dihydroartemisinin Supplementation Alleviates Intestinal Inflammatory Injury Through TLR4/NODs/NF-κB Signaling Pathway in Weaned Piglets with Intrauterine Growth Retardation

Dietary fermented soybean meal replacement alleviates diarrhea in weaned piglets challenged with enterotoxigenic Escherichia coli K88 by modulating inflammatory cytokine levels and cecal microbiota composition

Comparison of the protective effects of resveratrol and pterostilbene against intestinal damage and redox imbalance in weanling piglets

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