Adherence of Erythrocytes to Stratum corneum of Skin Tissue Sections

Krogh, H.K.; Tönder, O.

doi:10.1159/000230107

Cited by 28 publications

(54 citation statements)

References 5 publications

(7 reference statements)

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“…Callus was obtained from an individual with congenital hyperkeratosis of the palms and soles. Sediment of the tissue was prepared as described earlier [7]. Microtome sections of frozen human skin from adults were obtained and processed as previously reported [6,7], Extraction of tissue.…”

Section: Methodsmentioning

confidence: 99%

“…Sediment of the tissue was prepared as described earlier [7]. Microtome sections of frozen human skin from adults were obtained and processed as previously reported [6,7], Extraction of tissue. Lyophilized tissue was extracted with PW as earlier de scribed [5].…”

Section: Methodsmentioning

confidence: 99%

“…Sheep erythrocytes were obtained and pre pared as reported earlier [7]. Sensitization of erythrocytes was performed as de scribed [10], The sensitizing activity of the various preparations was determined by a checkerboard technique.…”

Section: Methodsmentioning

confidence: 99%

“…For removal of isoantibodies, serum diluted 1 in 4 was absorbed at 20 °C for 30 min with packed, human AB Rh-positive erythrocytes or erythrocytes from the do nor of callus as described earlier [7].…”

Section: Methodsmentioning

confidence: 99%

“…Originally, these antibodies were demonstrated using the immune adherence test with microtome sections of human skin [7], and later by fluorescent antibody and indirect haemag glutination techniques [5,10]. The antigen used for sensitization of eryth rocytes was prepared by extraction of homogenates of callus with phenol water (PW) and recovered from the water phase.…”

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confidence: 99%

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Heterogeneity of Antigen from Callus of Man

Mæland¹,

Krogh²,

Tönder³

1974

Int Arch Allergy Immunol

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The question of whether one or more antigens are involved in the reaction between antibodies in human serum and callus of man was investigated. Callus was collected from an individual with congenital hyperkeratosis. Preparations were obtained by extraction of homogenated callus with (1) phenol water (PW), and (2) the combined use of trypsin digestion and phenol water extraction (TPW). The phenol and water phases were studied using indirect haemagglutination technique. All preparations sensitized erythrocytes for agglutination by human serum except for the phenol phase of the PW preparation. Absorption and inhibition experiments showed that one antigen present in the TPW water phase was also present in the PW water phase. In addition, the PW water phase contained another antigen. In the phenol phase of the TPW preparation a third antigen was demonstrated. Serological cross-reactivity between the antigens and bacteria isolated from callus was not demonstrated. The results of immune adherence test with sections of human skin and unabsorbed or absorbed human serum indicated that the two water phase antigens are present in normal human skin.

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Section: Methodsmentioning

confidence: 99%