Natural Stratum Corneum Antibodies and Antigens

Jc, Bystryn

doi:10.1007/978-3-642-68682-5_13

Cited by 3 publications

(3 citation statements)

References 23 publications

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“…18 However IgG antibodies are present in the newborns' skin during the first 2 months of life, passively transferred from the mother. 19 In contrast to all the numerous and sometimes farfetched investigations of the epidermic cells of the fetus and of the adult, the cells of the VC have been very poorly examined, especially in regard to their particular morphologic and functional characteristics. In an attempt to collect more information about the features of this relatively unexplored material, we investigated some of the just mentioned histochemical (acid and alkaline phosphatase activity), ultrastructural (as viewed by scanning and transmission electron microscope) and immunologic (IgG, IgM, IgA, and C3 presence) characteristics of the VC cells.…”

mentioning

confidence: 98%

Features of Vernix Caseosa Cells

Αγοραστός

Hollweg²,

Grussendorf³

et al. 1988

Amer J Perinatol

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In an attempt to collect more information about the features of the vernix caseosa (VC), a relatively unstudied material, some of the histochemical, ultrastructural, and immunological characteristics of VC cells have been investigated. Histochemistry and light microscopy was used to demonstrate the activity of acid and alkaline phosphatase in VC cells, enzymes with a marked increase in activity in the amniotic fluid toward term. Acid phosphatase activity was strongly present either as intracytoplasmic granules or as amorphous material between the cells; alkaline phosphatase activity was absolutely nonexistent. The ultrastructural morphology of the VC cells was analyzed by scanning and transmission electron microscopy (TEM). Significant differences can be demonstrated in the individual surface patterns of the VC keratinocytes. TEM showed irregularly flattened cells in various stages of keratinization. The ultrastructural findings confirm the dissimilarity, which exists between the individual VC cells. Finally, immunofluorescent staining tests of frozen VC smears showed that only immunoglobulin G conjugate gives strong positive reaction at the antigen sites of the VC cells. The special finding in this study is the polymorph appearance of the surface pattern and the cytoplasma structure of the VC cells, as well as the lack of uniform appearance of the acid phosphatase activity in and between the cells. All these suggest that the status of the individual VC cell is not similar in regard to their keratinization and desquamation activities.

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mentioning

confidence: 98%

Features of Vernix Caseosa Cells

Αγοραστός

Hollweg²,

Grussendorf³

et al. 1988

Amer J Perinatol

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“…Both the insoluble glycoprotein SC antigen reactive in IIF [4,5] and the soluble, carbo hydrate SC antigen reactive in passive hemagglutina tion and immune adherence [12,[14][15][16] were studied. In both systems tissue but not species specificity was found.…”

Section: Discussionmentioning

confidence: 99%

“…Existence of allospecificity among SC antigens was suspected previously because of the fact that the SCAb titer of one serum may vary considerably on epidermal substrates from different individuals [5]. Further evidence to support this thesis was provided by the observation that absorption of SCAb from hu man serum by ground callus from a single donor abolishes the IIF reaction of this serum on some but not all substrates from different subjects [7], In this work we compared the substrate specifici ties of monkey and human SCAb by examining the capacity of the former to interfere with the reaction of the latter on the same epidermal substrate.…”

Section: Introductionmentioning

confidence: 99%

Demonstration of Allospecific Differences in Human Stratum corneum Autoantibodies by Blocking Immunofluorescence

Dabski

Beutner

1985

Int Arch Allergy Immunol

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Stratum corneum antibody titers of six normal human sera were determined by indirect immunofluorescence on three different epidermal substrates with and without preincubation of the substrates with one monkey serum. In three out of six human sera the reaction of stratum corneum antibodies was inhibited on all three substrates, in one serum the inhibition was noted on two out of three substrates and no effect was observed in two sera. These findings provide further evidence for the existence of allospecific stratum corneum antibodies in human sera and for the presence of both species-specific and species-nonspecific glycoprotein stratum corneum antigens in human epidermis.

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Stratum corneum antibodies detected by hemagglutination are not directed against keratin intermediate filaments

et al. 1990

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Autoantibodies to stratum corneum (SC) occur in virtually all normal adult human sera. These antibodies may be directed against various antigens of the SC. They have been detected by indirect immunofluorescence, passive hemagglutination (HA), immune adherence, and most recently by enzyme immunoassay and immunoblot methods. The purpose of our study was to examine whether antibodies to SC antigens as detected by passive HA are similar to the keratin intermediate filament (KIF) reactive antibodies. SC antigen preparation was prepared from psoriatic scales by the trypsin-phenol-water (TPW) extraction method. KIFs were prepared by 8 M urea extraction of normal callus or psoriatic scales. The anti-SC antibody titers of normal human sera were determined by passive HA before and after absorption with TPW-SC antigen preparation and upon absorption with KIFs. Similarly, titers of anti-KIF antibodies were determined on absorbed and unabsorbed sera by immunoblot assay. The results of this study indicate that the absorption of the sera with KIFs did not affect the titer of antibodies to TPW-extractable SC antigens whereas the titer of KIF antibodies dropped. KIF-reactive antibodies, on the other hand, were not affected by absorption with TPW-SC antigen, whereas the latter absorbed out the corresponding reactive antibodies. These results indicate that antibodies directed against SC antigen are different from the KIF-reactive antibodies.

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Natural Stratum Corneum Antibodies and Antigens

Cited by 3 publications

References 23 publications

Features of Vernix Caseosa Cells

Features of Vernix Caseosa Cells

Demonstration of Allospecific Differences in Human Stratum corneum Autoantibodies by Blocking Immunofluorescence

Stratum corneum antibodies detected by hemagglutination are not directed against keratin intermediate filaments

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