1974
DOI: 10.1159/000231162
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Heterogeneity of Antigen from Callus of Man

Abstract: The question of whether one or more antigens are involved in the reaction between antibodies in human serum and callus of man was investigated. Callus was collected from an individual with congenital hyperkeratosis. Preparations were obtained by extraction of homogenated callus with (1) phenol water (PW), and (2) the combined use of trypsin digestion and phenol water extraction (TPW). The phenol and water phases were studied using indirect haemagglutination technique. All preparations sensitized erythrocytes f… Show more

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Cited by 12 publications
(15 citation statements)
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References 8 publications
(32 reference statements)
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“…Similar reactivity and cross-reactivity of sera was previously reported [11] and verified in this study using the immune ad herence test with skin sections. The agreement between the results ob tained in the two tests again indicates that the antibodies active in indi rect haemagglutination are also involved in the immune adherence test [16]. The cross-reactivity of sera could be mediated either by antibodies to common antigenic determinants, antibodies to species-specific deter minants or to both.…”
Section: Discussionmentioning
confidence: 54%
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“…Similar reactivity and cross-reactivity of sera was previously reported [11] and verified in this study using the immune ad herence test with skin sections. The agreement between the results ob tained in the two tests again indicates that the antibodies active in indi rect haemagglutination are also involved in the immune adherence test [16]. The cross-reactivity of sera could be mediated either by antibodies to common antigenic determinants, antibodies to species-specific deter minants or to both.…”
Section: Discussionmentioning
confidence: 54%
“…We reported previously [16] that in addition to the TPW water phase, the PW water and TPW phenol phases from callus contain ery throcyte-sensitizing antigens. These findings were verified in this study.…”
Section: Discussionmentioning
confidence: 99%
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“…More aggressive methods are needed to disrupt the horny layer structure and to ex pose its normally hidden components capable of reacting with SC antibodies in indirect hemagglutina tion, i.e., the method described by Krogh et al [17], combining proteolytic digestion with trypsin and sub sequent extraction with phenol and water (the socalled trypsin-phenol water method). Absorption and hemagglutination inhibition studies of Maelandet al [21] and Krogh and Tender [20] give evidence that at least three different SC antigens reacting in indirect hemagglutination are present in callus. Several au thors reported on possible differences in the chemical composition existing between normal horny layer and callus [8,9], By comparison of water and phenol phases obtained from phenol-water and trypsin-phe nol water extractions, serologic differences between SC, callus and psoriatic scales were also found by Krogh and Tonder [20], None of the antigenic prepa rations obtained by trypsin-phenol water methods is active against SC antibodies detectable by indirect IF on normal human skin, normal monkey skin, or the cut edge of callus, unless the latter is degraded by treatment with a crude trypsin preparation (contain ing both trypsin and chymotrypsin) as shown by the present studies.…”
Section: Discussionmentioning
confidence: 97%