Adenovirus, calicivirus and astrovirus detection in fecal samples of hospitalized children with acute gastroenteritis from Campo Grande, MS, Brazil

Andreasi, Márcia Sueli Assis; Cardoso, Divina das Dôres de Paula; Fernandes, Sonia Maria; Tozetti, Inês Aparecida; Borges, Ana Maria Tavares; Fiaccadori, Fabíola Souza; Santos, Rodrigo Alessandro Tôgo; Souza, Menira

doi:10.1590/s0074-02762008000700020

Cited by 46 publications

(43 citation statements)

References 22 publications

(24 reference statements)

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“…Based on the mean occurrence of other gastroenteric viruses in the region, HBoV-1 could be considered the third most common after RVAs and human cali-civiruses (Cardoso et al 2003, Costa et al 2004, Borges et al 2006, Andreasi et al 2008. Additionally, it must be noted that primer pairs that detect only HBoV species 1 and 3 were used in this study and if one considers that species 2 appears to be predominant in individuals with gastroenteritis (Arthur et al 2009, Chow et al 2010, the observed positivity rate for HBoVs may be underestimated.…”

Section: Discussionmentioning

confidence: 91%

“…In addition, in the Central-West Region of Brazil, many studies have focused on the investigation of other gastroenteric agents in this population (Cardoso et al 1989, 2003, Camarota et al 1992, Borges et al 2006, Oh et al 2006, Andreasi et al 2008, Silva et al 2009). Therefore, the objective of this study, the first conducted in this region, was the detection of HBoV-1 and HBoV-3 in faecal samples obtained from children under five years of age presenting with acute gastroenteritis.…”

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confidence: 99%

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Human bocavirus 1 and 3 infection in children with acute gastroenteritis in Brazil

Sousa¹,

Souza²,

Fiaccadori³

et al. 2012

Mem. Inst. Oswaldo Cruz

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To determine the positivity rate of human bocavirus (HBoV) 1 and 3 among children who presented with acute gastroenteritis symptoms during the period of 1994-2004 in the Central-West Region of Brazil, 762 faecal samples were tested using polymerase chain reaction (PCR) for the detection of HBoV DNA. Primers for a segment of the non-structural viral protein 1 (NS1) gene of HBoV-1 and HBoV-3 were used. Twelve HBoV-positive samples were further characterised via genomic sequencing and phylogenetic analysis. Of the samples tested, 5.8% (n = 44) were positive for HBoV-1 or HBoV-3 and co-infection was observed in 14 (31.8%) of the 44 HBoV-positive samples. Nine of the 14 samples were also positive for Rotavirus A and five were positive for Aichi virus. The genomic sequencing of the NS1 partial sequence of 12 HBoV-samples showed that 11 samples were characterised as HBoV-1 and that one was characterised as HBoV-3. The phylogenetic analysis showed that the HBoV-1 samples had a high sequence homology to others previously identified in China, Sweden and Brazil. This is the first study conducted in the Central-West Region of Brazil to detect HBoV-1 and HBoV-3 in faecal samples from children with acute gastroenteritis. Further studies are required to define the role of HBoVs as aetiological agents of gastroenteritis

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Section: Discussionmentioning

confidence: 91%

mentioning

confidence: 99%

Human bocavirus 1 and 3 infection in children with acute gastroenteritis in Brazil

Sousa¹,

Souza²,

Fiaccadori³

et al. 2012

Mem. Inst. Oswaldo Cruz

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“…The infections typically occur sporadically, and the cause of 2 to 10% of total viral gastroenteritis cases is attributed to HAstVs (1)(2)(3)(4)(5)(6). Astroviruses (AstVs) possess nonenveloped, icosahedrally shaped virions containing approximately 6,800 nucleotides (nt) of single-stranded positive-sense RNA (7).…”

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confidence: 99%

Wastewater Analysis Indicates that Genetically Diverse Astroviruses, Including Strains Belonging to Novel Clades MLB and VA, Are Circulating within Japanese Populations

Hata

Katayama

Kitajima

et al. 2015

Appl Environ Microbiol

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Human astroviruses (HAstVs) are a common etiological agent of infantile gastroenteritis. Recent studies revealed that novel astrovirus (AstV) strains of the MLB clade (MLB-AstVs) and VA clade (VA-AstVs), which are genetically distinct from the classic HAstVs, are circulating in the human population. In the present study, we quantified classic HAstVs as well as carried out a genetic analysis of classic and novel HAstVs in wastewater in Japan. The concentration of classic HAstVs in the influent water samples ranged from 10 4 to 10 5 copies per liter, and the amount removed by wastewater treatment was determined to be 2.4 ؎ 0.3 log 10 . Four types of classic HAstV strains (HAstV types 1, 2, 5, and 4/8) as well as novel AstV strains belonging to the MLB-2, VA-1, and VA-2 clades were identified using reverse transcription-PCR (RT-PCR) assays, including assays newly developed for the detection of strains of the MLB and VA clades, followed by cloning and nucleotide sequencing. Our results suggest that genetically diverse AstV strains are circulating among the human population in Japan. The newly developed (semi)nested RT-PCR assays for these novel AstV clades are useful to identify and characterize the novel AstVs in environmental waters.H uman astroviruses (HAstVs) are considered one of the most important causes of infantile gastroenteritis worldwide (1). The infections typically occur sporadically, and the cause of 2 to 10% of total viral gastroenteritis cases is attributed to HAstVs (1-6). Astroviruses (AstVs) possess nonenveloped, icosahedrally shaped virions containing approximately 6,800 nucleotides (nt) of single-stranded positive-sense RNA (7). It has been reported that the diameter of AstV particles shed in fecal specimens ranges from 28 to 31 nm, while the diameter of those grown in cell culture is 41 nm (8). Their viral genome consists of three open reading frames (ORFs), namely, ORF1a, ORF1b, and ORF2, encoding the serine protease, RNA-dependent RNA polymerase, and capsid proteins, respectively (7). HAstVs are antigenically or genetically classified into eight different types (HAstV type 1 [HAstV-1] to HAstV-8). Previous studies revealed that HAstV-1 is the most prevalent type among infected individuals and in the environment, followed by 5,[9][10][11][12][13].Because HAstV can be excreted in the feces of infected individuals at a high concentration (up to 10 15 particles/g) (14), examination of municipal wastewater samples could be an effective approach to understand the actual prevalence and epidemiology of these viruses (15, 16). In spite of their importance as enteric pathogens, the occurrence and other characteristics of HAstVs in water compared to those of other enteric viruses, such as noroviruses, rotaviruses, and adenoviruses, have not been well documented (17, 18). Due to the scarcity of knowledge, the fate of HAstVs in water environments and wastewater treatment processes is not well understood.Recent studies based on viral metagenomic analysis have identified some novel AstVs, namely, clade MLB ...

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“…In Brazil, some investigations have reported HAdV and SaV infections among hospitalized children in the Northern Region, with prevalence rates of 2-10% and 2.5-5.9%, respectively, based on enzyme immunoassay (EIA), PCR, and RT-PCR methodologies [8][9][10][11] . With the advent of molecular techniques, it is now possible to detect and genotype enteric viruses, and thus improve our knowledge of their molecular epidemiological profiles.…”

Section: Introductionmentioning

confidence: 99%

Detection and genotyping of human adenovirus and sapovirus in children with acute gastroenteritis in Belém, Pará, between 1990 and 1992: first detection of GI.7 and GV.2 sapoviruses in Brazil

Costa

Siqueira

Portal

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Introduction: Acute gastroenteritis (AGE) is one of the most common causes of morbidity and mortality, especially among children from developing countries. Human adenovirus (HAdV) and sapovirus (SaV) are among the agents that cause AGE. The present study aimed to detect and genotype HAdV and SaV in 172 fecal samples from children with AGE, collected during a surveillance study carried out in a low-income community in Belém, Pará, between 1990 and 1992. Methods: HAdV was detected by nested PCR, using primers Hex1deg/Hex2deg and NeHex3deg/NeHex4deg. SaV was assayed by reverse transcription PCR (RT-PCR), nested PCR, and quantitative PCR. The nucleotide sequence was determined by direct cycle sequencing. Results: Overall, 43% (74/172) of samples were positive for HAdV, of which 70.3% (52/74) were sequenced and classified as belonging to five different species, mostly A and F. For SaV, positivity was 5.2% (9/172) and genotypes GI.1, GI.7, GII.1, and GV.2 were detected. Conclusions: The present results reinforce the need for further studies to obtain epidemiological data about the circulation of these viruses in Brazil, especially in the Amazon Region, where data from the early 1990's are scarce. Furthermore, the study describes for the first time the detection of SaV genotypes GI.7 and GV.2 in Brazil, showing that these types circulated in the region more than 25 years ago.

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Adenovirus, calicivirus and astrovirus detection in fecal samples of hospitalized children with acute gastroenteritis from Campo Grande, MS, Brazil

Cited by 46 publications

References 22 publications

Human bocavirus 1 and 3 infection in children with acute gastroenteritis in Brazil

Human bocavirus 1 and 3 infection in children with acute gastroenteritis in Brazil

Wastewater Analysis Indicates that Genetically Diverse Astroviruses, Including Strains Belonging to Novel Clades MLB and VA, Are Circulating within Japanese Populations

Detection and genotyping of human adenovirus and sapovirus in children with acute gastroenteritis in Belém, Pará, between 1990 and 1992: first detection of GI.7 and GV.2 sapoviruses in Brazil

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